PMID:4887874: Difference between revisions

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!align=left  |Citation
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'''Grodzicker, T and Zipser, D'''  (1968) A mutation which creates a new site for the re-initiation of polypeptide synthesis in the z gene of the lac operon of Escherichia coli.''J. Mol. Biol.'' '''38''':305-14
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!align=left  |Abstract
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No abstract in PubMed
|-
!align=left  |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4887874 PubMed]
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!align=left  |Keywords
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Polar Effect; Acyltransferases; Chromosome Mapping; Escherichia coli; Genes; Membrane Transport Proteins; Mutation; Operon; Translation; Suppressor
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==Main Points of the Paper ==
*Polar effects of a UAG mutation in the operator-proximal region of the ''z'' gene are studied which leads to the isolation of two suppressor mutations
*π, a new site of initiation of protein synthesis is described
**operates efficiently to restore permease and acetylase activity to a high level when induced
**regulation/induction is still required, so the authors deduce that π is a new site of translation initiation (
**not a new site of transcriptional initiation because acetylase is not present in the absence of inducer
== Materials and Methods Used ==
*Parent Strain: NG545 (''trp-8'', Sm<sup>S</sup> Hfr)
**UAG mutation had not been altered in the suppressor strains isolated
*Strain: CA77B (''Δlac, trp-8'', Sm<sup>R</sup> F<sup>-</sup>)
*Strain: X103B (''Δlac pro, trp-8'', Sm<sup>R</sup> F<sup>-</sup>)
*Strain: M107 (''Δlac, su<sub>I</sub><sup>+</sup>'', Sm<sup>R</sup> F<sup>-</sup>)
*Strain: ΔLac<sub>W</sub> (''Δlac, his<sub>-</sub>'', Sm<sup>R</sup> F<sup>-</sup>)
*Strain: E29 (''lac<sup>+</sup> '', F'/''lac<sup>+</sup>'', threonine<sup>-</sup>, leucine<sup>-</sup>, B<sub>1</sub><sup>-</sup>, Sm<sup>R</sup>)
*Acetylase assay
*β-galactosidase assay
==Phenotype Annotations==
{{AnnotationTableHelp}}
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!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
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''Escherichia coli''
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NCBI:562
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NG545-pi(1)<sub>a</sub>
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lacZ
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*Growth on melibiose medium
*Melibiose catabolic process
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Growth
|
growth on melibiose- polar effects
|
Plating Assay
|
Table 2 
|-
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''Escherichia coli''
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NCBI:562
|
NG545-pi(1)
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lacZ
|
*Suppressor-dependent growth on lactose medium
*Suppressor-dependent lactose catabolic process
|
Growth
|
able to metabolize lactose only in a su<sub>I</sub><sub>+</sub> background
|
Plating Assay
|
Table 2- UAG polar mutation
|-
|
''Escherichia coli''
|
NCBI:562
|
NG545-pi(1)<sub>b</sub>
|
lacZ
|
*Abolished melibiose catabolic process
*Absent growth on melibiose medium
|
Growth
|
unable to metabolize melibiose/ growth on melibiose-polar effects
|
Plating Assay
|
Table 2- 
|-
|
''Escherichia coli''
|
NCBI:562
|
NG545-pi(1)<sub>a</sub>
|
lacZ
|
*Decreased gene expression of beta-galactosidase
*Decreased lactose catabolic process
|
Metabolic Activity
|
trace amounts when induced
|
Biochemical Assay
|
Table 2 
|-
|
''Escherichia coli''
|
NCBI:562
|
NG545-pi(1)<sub>b</sub>
|
lacZ
|
reduced beta-galactosidase activity
|
Metabolic Activity
|
trace amounts when induced
|
Biochemical Assay
|
Table 2 
|-
|
''Escherichia coli''
|
NCBI:562
|
NG545B
|
lacZ
|
*Decreased acetylase activity
*Decreased lactose catabolic process
|
Metabolic Activity
|
4.2% of the wild type level when induced
|
Biochemical Assay
|
Tables 3 and 4 
|-
|
''Escherichia coli''
|
NCBI:562
|
NG545
|
lacZ
|
*Suppressor-dependent growth on lactose medium
*Suppressor-dependent lactose catabolic process
|
Growth
|
able to metabolize lactose only in a su<sub>I</sub><sub>+</sub> background
|
Plating Assay
|
Table 2- UAG polar mutation 
|-
|
''Escherichia coli''
|
NCBI:562
|
NG545-pi(1)
|
lacZ
|
*Decreased acetylase activity
*Decreased lactose catabolic process
|
Metabolic Activity
|
35% of the wild type level when induced
|
Biochemical Assay
|
Tables 3 and 4 
|-
|
''Escherichia coli''
|
NCBI:562
|
NG24
|
lacZ
|
*Decreased acetylase activity
*Decreased lactose catabolic process
|
Metabolic Activity
|
4.7% of the wild type level when induced
|
Biochemical Assay
|
Table 4 
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==Notes==
==References==
{{RefHelp}}
<references/>
[[Category:Publication]]
[[Category:To Be Converted]]

Latest revision as of 17:09, 22 June 2011

Citation

Grodzicker, T and Zipser, D (1968) A mutation which creates a new site for the re-initiation of polypeptide synthesis in the z gene of the lac operon of Escherichia coli.J. Mol. Biol. 38:305-14

Abstract

No abstract in PubMed

Links

PubMed

Keywords

Polar Effect; Acyltransferases; Chromosome Mapping; Escherichia coli; Genes; Membrane Transport Proteins; Mutation; Operon; Translation; Suppressor

edit table

Main Points of the Paper

  • Polar effects of a UAG mutation in the operator-proximal region of the z gene are studied which leads to the isolation of two suppressor mutations
  • π, a new site of initiation of protein synthesis is described
    • operates efficiently to restore permease and acetylase activity to a high level when induced
    • regulation/induction is still required, so the authors deduce that π is a new site of translation initiation (
    • not a new site of transcriptional initiation because acetylase is not present in the absence of inducer

Materials and Methods Used

  • Parent Strain: NG545 (trp-8, SmS Hfr)
    • UAG mutation had not been altered in the suppressor strains isolated
  • Strain: CA77B (Δlac, trp-8, SmR F-)
  • Strain: X103B (Δlac pro, trp-8, SmR F-)
  • Strain: M107 (Δlac, suI+, SmR F-)
  • Strain: ΔLacW (Δlac, his-, SmR F-)
  • Strain: E29 (lac+ , F'/lac+, threonine-, leucine-, B1-, SmR)
  • Acetylase assay
  • β-galactosidase assay

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.

Species Taxon ID Strain Gene (if known) OMP Phenotype Details Evidence Notes

Escherichia coli

NCBI:562

NG545-pi(1)a

lacZ

  • Growth on melibiose medium
  • Melibiose catabolic process

Growth

growth on melibiose- polar effects

Plating Assay

Table 2

Escherichia coli

NCBI:562

NG545-pi(1)

lacZ

  • Suppressor-dependent growth on lactose medium
  • Suppressor-dependent lactose catabolic process

Growth

able to metabolize lactose only in a suI+ background

Plating Assay

Table 2- UAG polar mutation

Escherichia coli

NCBI:562

NG545-pi(1)b

lacZ

  • Abolished melibiose catabolic process
  • Absent growth on melibiose medium

Growth

unable to metabolize melibiose/ growth on melibiose-polar effects

Plating Assay

Table 2-

Escherichia coli

NCBI:562

NG545-pi(1)a

lacZ

  • Decreased gene expression of beta-galactosidase
  • Decreased lactose catabolic process

Metabolic Activity

trace amounts when induced

Biochemical Assay

Table 2

Escherichia coli

NCBI:562

NG545-pi(1)b

lacZ

reduced beta-galactosidase activity

Metabolic Activity

trace amounts when induced

Biochemical Assay

Table 2

Escherichia coli

NCBI:562

NG545B

lacZ

  • Decreased acetylase activity
  • Decreased lactose catabolic process

Metabolic Activity

4.2% of the wild type level when induced

Biochemical Assay

Tables 3 and 4

Escherichia coli

NCBI:562

NG545

lacZ

  • Suppressor-dependent growth on lactose medium
  • Suppressor-dependent lactose catabolic process

Growth

able to metabolize lactose only in a suI+ background

Plating Assay

Table 2- UAG polar mutation

Escherichia coli

NCBI:562

NG545-pi(1)

lacZ

  • Decreased acetylase activity
  • Decreased lactose catabolic process

Metabolic Activity

35% of the wild type level when induced

Biochemical Assay

Tables 3 and 4

Escherichia coli

NCBI:562

NG24

lacZ

  • Decreased acetylase activity
  • Decreased lactose catabolic process

Metabolic Activity

4.7% of the wild type level when induced

Biochemical Assay

Table 4

edit table


Notes

References

See Help:References for how to manage references in OMPwiki.

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