PMID:4905670: Difference between revisions

From OMPwiki
Jump to navigationJump to search
Table edited by Azweifel via TableEdit
imported>Wikientrybot
change PMID template to TableEdit version
 
(4 intermediate revisions by 2 users not shown)
Line 1: Line 1:
{{RightTOC}}
{{RightTOC}}
 
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.176.I5223848eaa50f-->
<!--box uid=2ccfb3c7bf1208312f02a69e64bfd9e0.176.D4d067c6d1367f-->
<!--
<!--
******************************************************************************************
******************************************************************************************
Line 8: Line 7:
*  
*  
****************************************************************************************** -->
****************************************************************************************** -->
{|  id="D4d067c6d1367f"  class=" tableEdit PMID_info_table"   
{|  id="I5223848eaa50f"  class=" tableEdit PMID_info_table"   


|-
|-
!align=left |Citation
!align=left align='left' bgcolor='#CCCCFF' |Citation
||
||
'''Wong, PT, Kashket, ER and Wilson, TH'''  (1970) Energy coupling in the lactose transport system of Escherichia coli.''Proc. Natl. Acad. Sci. U.S.A.'' '''65''':63-9
'''No author listed'''  (1970) Energy coupling in the lactose transport system of Escherichia coli. ''Proc. Natl. Acad. Sci. U.S.A.'' '''65''':63-9
|-
|-
!align=left |Abstract
!align=left align='left' bgcolor='#CCCCFF' |Abstract
||
||
A mutant (ML 308-22) was isolated from Escherichia coli ML 308, which had lost the normal capacity to accumulate lactose analogs despite an increase in the membrane carrier activity. The exit of thiomethylgalactoside was much faster than normal, accounting for the inability of the cell to maintain high intracellular concentrations of galactosides. Growth of the mutant on lactose was normal at high concentrations of sugar and impaired at low concentrations. This transport defect appeared to be limited to the lactose transport system as D-fucose and alpha-aminoisobutyric acid uptake and accumulation were normal. It is inferred from the data that the mutant possessed a defect in the coupling of metabolic energy to lactose transport.
A mutant (ML 308-22) was isolated from Escherichia coli ML 308, which had lost the normal capacity to accumulate lactose analogs despite an increase in the membrane carrier activity. The exit of thiomethylgalactoside was much faster than normal, accounting for the inability of the cell to maintain high intracellular concentrations of galactosides. Growth of the mutant on lactose was normal at high concentrations of sugar and impaired at low concentrations. This transport defect appeared to be limited to the lactose transport system as D-fucose and alpha-aminoisobutyric acid uptake and accumulation were normal. It is inferred from the data that the mutant possessed a defect in the coupling of metabolic energy to lactose transport.
|-
|-
!align=left |Links
!align=left align='left' bgcolor='#CCCCFF' |Links
||
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4905670 PubMed]
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4905670 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC286191 PMC286191]


|-
|-
!align=left |Keywords
!align=left align='left' bgcolor='#CCCCFF' |Keywords
||
||
Aminoisobutyric Acids; Biological Transport, Active; Carbon Isotopes; Escherichia coli; Fucose; Genetics, Microbial; Glycosides; Lactose; Mutation
Aminoisobutyric Acids/metabolism; Biological Transport, Active; Carbon Isotopes; Escherichia coli/isolation & purification; Escherichia coli/metabolism; Fucose/metabolism; Genetics, Microbial; Glycosides/metabolism; Lactose/metabolism; Mutation


|- class="tableEdit_footer"  
|- class="tableEdit_footer"  
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=2ccfb3c7bf1208312f02a69e64bfd9e0.176.D4d067c6d1367f&page=176&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> ||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.176.I5223848eaa50f&page=176&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> ||
|}
|}
<!--box uid=2ccfb3c7bf1208312f02a69e64bfd9e0.176.D4d067c6d1367f-->
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.176.I5223848eaa50f-->
 
==Main Points of the Paper ==
==Main Points of the Paper ==
{{LitSignificance}}
{{LitSignificance}}


== Materials and Methods Used ==
== Materials and Methods Used ==
* Parent Strain: ML308 (''i<sup>-</sup>z<sup>+</sup>y<sup>+</sup>a<sup>+</sup>
{{LitMaterials}}
 
*ONPG hydrolysis assay (measure of transport carriers)
*β-galactoside uptake assays
*Counterflow experiments
*M protein (LacY) assay


==Phenotype Annotations==
==Phenotype Annotations==
<protect><!--box uid=2ccfb3c7bf1208312f02a69e64bfd9e0.176.U4d067c6d14b4c-->
{{AnnotationTableHelp}}
<protect><!--box uid=d41d8cd98f00b204e9800998ecf8427e.176.N5223848ed4612-->
<!--
<!--
******************************************************************************************
******************************************************************************************
Line 52: Line 46:
*  
*  
****************************************************************************************** -->
****************************************************************************************** -->
{|   id="U4d067c6d14b4c"  class=" tableEdit PMID_Phenotype_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="N5223848ed4612"  class=" tableEdit Phenotype_Table_2"   
|-
|- align='left' bgcolor='#CCCCFF'
!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
|-
|
''Escherichia coli''
|
NCBI:562
|
ML308-22
|
''lacY<sup>UN</sup>''
|
severely reduced accumulation of beta-galactosides
|
Other
|
possesses 1/10 of the active transport capacity of the parent based on intracellular [<sup>14</sup>C]TMG concentration
|
Other
|
Figure 1- Filter assay
|-
|
''Escherichia coli''
|
NCBI:562
|
ML308-22
|
''lacY<sup>UN</sup>''
|
increased number of transporters
|
Metabolic Activity
|
The release of ''o''-nitrophenol from ONPG by washed mutant was 153% of that f the parent
|
Other
|
Table 2- ONPG hydrolysis is a measure of membrane carriers for lactose
|-
|
''Escherichia coli''
|
NCBI:562
|
ML308-22
|
''lacY<sup>UN</sup>''
|
increased number of transporters
|
Metabolic Activity
|
The mutant showed a more rapid initial rate of entry
|
Other
|
Figure 2- TMG counterflow assay
|-
|
''Escherichia coli''
|
NCBI:562
|
ML308-22
|
''lacY<sup>UN</sup>''
|
increased beta-galactosidase activity
|
Metabolic Activity
|
The cell-free extract of the mutant contained 147% of the beta-galactosidase activity of that of the parent
|
Other
|
in text, pg. 66
|-
|
''Escherichia coli''
|
NCBI:562
|
ML308-22
|
''lacY<sup>UN</sup>''
|
increased binding of substrate
|
Other
|
[<sup>3</sup>H]TDG binding is increased 163% compared to the parent
|
Other
|
Table 3- [<sup>3</sup>H]TDG binding assay 
|-
|
''Escherichia coli''
|
NCBI:562
|
ML308-22
|
''lacY<sup>UN</sup>''
|
reduced growth rate at lower lactose concentrations
|
Growth
|
At an external lactose concentration of 0.5mM, the mutant had a longer doubling time and at 0.25mM lactose, the cells did not grow
|
Other
|
Table 4- evaluation of doubling times at varying lactose concentrations 
|-
|
''Escherichia coli''
|
NCBI:562
|
ML308-22
|
''lacY<sup>UN</sup>''
|
increased rate of sugar efflux
|
Other
|
 
|
Other
|
Figure 4- [<sup>UN</sup>C]TMG filter assay


|- class="tableEdit_footer"  
|- class="tableEdit_footer"  
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=2ccfb3c7bf1208312f02a69e64bfd9e0.176.U4d067c6d14b4c&page=176&pagename={{FULLPAGENAMEE}}&type=0&template=PMID_Phenotype_table edit table]</span> || || || || || || || ||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.176.N5223848ed4612&page=176&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</span> || || || || || || || || ||
|}
|}
<!--box uid=2ccfb3c7bf1208312f02a69e64bfd9e0.176.U4d067c6d14b4c--></protect>
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.176.N5223848ed4612--></protect>


==Notes==
==Notes==
==References==
{{RefHelp}}
<references/>
<references/>
[[Category:Publication]]

Latest revision as of 18:16, 1 September 2013

Citation

No author listed (1970) Energy coupling in the lactose transport system of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 65:63-9

Abstract

A mutant (ML 308-22) was isolated from Escherichia coli ML 308, which had lost the normal capacity to accumulate lactose analogs despite an increase in the membrane carrier activity. The exit of thiomethylgalactoside was much faster than normal, accounting for the inability of the cell to maintain high intracellular concentrations of galactosides. Growth of the mutant on lactose was normal at high concentrations of sugar and impaired at low concentrations. This transport defect appeared to be limited to the lactose transport system as D-fucose and alpha-aminoisobutyric acid uptake and accumulation were normal. It is inferred from the data that the mutant possessed a defect in the coupling of metabolic energy to lactose transport.

Links

PubMed PMC286191

Keywords

Aminoisobutyric Acids/metabolism; Biological Transport, Active; Carbon Isotopes; Escherichia coli/isolation & purification; Escherichia coli/metabolism; Fucose/metabolism; Genetics, Microbial; Glycosides/metabolism; Lactose/metabolism; Mutation

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status


Notes

References

See Help:References for how to manage references in OMPwiki.