Difference between revisions of "PMID:230407"
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*Taxon: Escherichia coli | *Taxon: Escherichia coli | ||
*Strain: K-12 | *Strain: K-12 | ||
| − | *Substrain: | + | *Substrain: |
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&p=mapview&p=has_linkout&p=blast_url&p=genome_blast&lin=f&keep=1&srchmode=1&unlock 83333] | *NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&p=mapview&p=has_linkout&p=blast_url&p=genome_blast&lin=f&keep=1&srchmode=1&unlock 83333] | ||
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| + | ECO:0000182 | ||
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| + | in vitro culture assay data | ||
| + | | | ||
| + | Deficient mutants in relA backgroumd, were found to be resistant to smg (serine+methionine+glycine) | ||
| + | | | ||
| + | |||
| + | |- | ||
| + | | | ||
| + | a mutation or genetic difference within a strain | ||
| + | | | ||
| + | *Taxon: Escherichia coli | ||
| + | *Strain: K-12 | ||
| + | *Substrain: BM6163 | ||
| + | *NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&p=mapview&p=has_linkout&p=blast_url&p=genome_blast&lin=f&keep=1&srchmode=1&unlock 83333] | ||
| + | | | ||
| + | *Genotype of Reference Strain: ''cya crp'' | ||
| + | *Genotype of Experimental Strain : ''cya(del) crp<nowiki>*</nowiki>'' | ||
| + | | | ||
| + | *Reference Condition: ''relA<sup> +</sup>'' | ||
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| − | + | ECO:0000182 | |
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| + | in vitro culture assay data | ||
| + | | | ||
| + | crp allele that modified cyclic AMP receptor proteins, which acts independently of cyclic AMP, is resistant to smg (serine+methionine+glycine) in a relA background. | ||
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==Notes== | ==Notes== | ||
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| + | waiting on new omp terms. Resistant to serine | ||
==References== | ==References== | ||
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| − | [[Category:Publication]] | + | [[Category:Publication]] [[Category:Papers referenced in the LaRossa chapter]] [[Category:LaRossa citations that need review]] |
Latest revision as of 18:14, 5 April 2012
| Citation |
Daniel, J and Danchin, A (1979) Involvement of cyclic AMP and its receptor protein in the sensitivity of Escherichia coli K 12 toward serine: excretion of 2-ketobutyrate, a precursor of isoleucine.Mol. Gen. Genet. 176:343-50 |
|---|---|
| Abstract |
A relationship between serine-induced growth sensitivity and the cAMP-CAP complex is established. Mutants of Escherichia coli K 12 deficient either in the cya or crp gene function exhibit a resistant phenotype on serine media although they harbor a relA allele normally leading to sensitivity toward serine. The presence of a crp allele in a cya delta relA background restores the sensitivity phenotype, while the analysis of serine resistant mutants selected from a crp cya delta relA strain shows that the mutation leading to resistance is located at, or very near, the crp gene, giving a more or less Crp- phenotype. In addition crp cya delta relA strains excrete large quantities of 2-ketobutyrate when grown on glucose M63 medium. This excretion is unambiguously linked to the presence of the crp allele and is correlated with an enhanced threonine deaminase activity. Besides, the complex regulation exerted on the acetolactate synthase activities is discussed. |
| Links | |
| Keywords |
Butyrates; Cyclic AMP; Escherichia coli; Isoleucine; Mutation; Phenotype; Receptors, Cyclic AMP; Serine; Transduction, Genetic |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
|
a mutation or genetic difference within a strain |
|
|
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ECO:0000182 |
in vitro culture assay data |
Deficient mutants in relA backgroumd, were found to be resistant to smg (serine+methionine+glycine) |
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|
a mutation or genetic difference within a strain |
|
|
|
ECO:0000182 |
in vitro culture assay data |
crp allele that modified cyclic AMP receptor proteins, which acts independently of cyclic AMP, is resistant to smg (serine+methionine+glycine) in a relA background. |
| ||
| edit table |
Notes
waiting on new omp terms. Resistant to serine
References
See Help:References for how to manage references in OMPwiki.
