OMP ST:947 ! DH1 miniFparC10: Difference between revisions

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==Strain Summary==
==Strain Summary==
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!align=left align='left' bgcolor='#CCCCFF' |Strain Name
||
DH1 miniFparC10
|-
!align=left align='left' bgcolor='#CCCCFF' |Synonyms
||
parC10 mutant
|-
!align=left align='left' bgcolor='#CCCCFF' |Taxon Information
||
*Pangenome: Escherichia coli
*Subspecies and/or strain: K-12
*NCBI Taxonomy ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
|-
!align=left align='left' bgcolor='#CCCCFF' |Genotype
||
 
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!align=left align='left' bgcolor='#CCCCFF' |Strain Reference
||
PMID:9077455
|-
!align=left align='left' bgcolor='#CCCCFF' |Strain availability
||
 
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!align=left align='left' bgcolor='#CCCCFF' |Ancestry
||
parent:DH1 miniFparC+
|-
!align=left align='left' bgcolor='#CCCCFF' |Annotated phenotypes
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==Phenotype Annotation==
==Phenotype Annotation==
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!|Annotation ID!!not!!OMP ID!!OMP term name!!Relative phenotype information!!Experimental condition!!ECO ID!!ECO term name!!Reference!!Annotation Extension!!Notes
|-
|
OMP_AN:725
|
 
|
OMP:0007392
|
viable cell
|
Relative to:
 
<hr>Genotype differences:<br>
Nothing to compare<br/>
<hr>Condition differences:<br>
Nothing to compare<br/>
|
*temperature:42C
*medium:Kanamycin
*medium:L agar
|
ECO:0000181
|
in vitro assay evidence
|
PMID:9077455
|
CHEBI:6104 Kanamycin
|
|-
|
OMP_AN:726
|
 
|
OMP:0007391
|
abolished viability
|
Relative to: OMP_AN:725
 
<hr>Genotype differences:<br>
no differences
<hr>Condition differences:<br>
medium:IPTG (100 uM)?
|
*temperature:42C
*medium:IPTG (100 uM)
*medium:Kanamycin
*medium:L agar
|
ECO:0000181
|
in vitro assay evidence
|
PMID:9077455
|
CHEBI:6104 Kanamycin
CHEBI:61448 IPTG
|
DNA nicking activity instead of transesterification
 
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Latest revision as of 21:16, 12 July 2016

Strain Summary

Strain Name

DH1 miniFparC10

Synonyms

parC10 mutant

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype
Strain Reference

PMID:9077455[1]

Strain availability
Ancestry

parent:DH1 miniFparC+

Annotated phenotypes




Notes

Phenotype Annotation

Annotation ID not OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:725

OMP:0007392

viable cell

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • temperature:42C
  • medium:Kanamycin
  • medium:L agar
ECO:0000181

in vitro assay evidence

PMID:9077455[1]

CHEBI:6104 Kanamycin

OMP_AN:726

OMP:0007391

abolished viability

Relative to: OMP_AN:725


Genotype differences:

no differences


Condition differences:

medium:IPTG (100 uM)?

  • temperature:42C
  • medium:IPTG (100 uM)
  • medium:Kanamycin
  • medium:L agar
ECO:0000181

in vitro assay evidence

PMID:9077455[1]

CHEBI:6104 Kanamycin CHEBI:61448 IPTG

DNA nicking activity instead of transesterification



Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ 1.0 1.1 1.2 Yokochi, T et al. (1996) DNA nicking by Escherichia coli topoisomerase IV with a substitution mutation from tyrosine to histidine at the active site. Genes Cells 1 1069-75 PubMed