Minimal medium of Spizizen: Difference between revisions

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{{Pagetop}}
{{Pagetop}}
== Purpose ==
== Purpose ==
This medium can be used to prepare naturally competent cells of ''Bacillus subtilis''.<ref name='PMID:16561900'/>


== Materials Needed ==
== Materials Needed ==

Latest revision as of 22:48, 23 June 2017

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Purpose

This medium can be used to prepare naturally competent cells of Bacillus subtilis.[1]

Materials Needed

Protocol

  • 0.2% ammonium sulfate
  • 1.4% dipotassium phosphate
  • 0.6% monopotassium phosphate
  • 0.1% sodium citrate (2H2O)
  • 0.02% magnesium sulfate (7H2O)
  • 0.5% glucose

Notes

Papers where this or a similar method has been used

<PMIDsummary /> PMID:4633157

Related Ontology Terms

References

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  1. ↑ Anagnostopoulos, C & Spizizen, J (1961) REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS. J. Bacteriol. 81 741-6 PubMed