Difference between revisions of "Minimal medium of Spizizen"
From OMPwiki
Andersonk282 (talk | contribs) (→Protocol) |
(→Purpose) |
||
| (2 intermediate revisions by one other user not shown) | |||
| Line 1: | Line 1: | ||
{{Pagetop}} | {{Pagetop}} | ||
== Purpose == | == Purpose == | ||
| + | This medium can be used to prepare naturally competent cells of ''Bacillus subtilis''.<ref name='PMID:16561900'/> | ||
== Materials Needed == | == Materials Needed == | ||
| Line 8: | Line 9: | ||
*1.4% dipotassium phosphate | *1.4% dipotassium phosphate | ||
*0.6% monopotassium phosphate | *0.6% monopotassium phosphate | ||
| − | *0.1% sodium citrate | + | *0.1% sodium citrate (2H<sub>2</sub>O) |
| − | *0.02% magnesium sulfate | + | *0.02% magnesium sulfate (7H<sub>2</sub>O) |
*0.5% glucose | *0.5% glucose | ||
| Line 16: | Line 17: | ||
== Papers where this or a similar method has been used == | == Papers where this or a similar method has been used == | ||
<PMIDsummary /> | <PMIDsummary /> | ||
| + | [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1212887/ PMID:4633157] | ||
== Related Ontology Terms == | == Related Ontology Terms == | ||
Latest revision as of 17:48, 23 June 2017
You can help OMP wiki by editing the content of this page. For information about becoming a registered user and obtaining editing privileges, see Help:Accounts.
Contents
Purpose
This medium can be used to prepare naturally competent cells of Bacillus subtilis.[1]
Materials Needed
Protocol
- 0.2% ammonium sulfate
- 1.4% dipotassium phosphate
- 0.6% monopotassium phosphate
- 0.1% sodium citrate (2H2O)
- 0.02% magnesium sulfate (7H2O)
- 0.5% glucose
Notes
Papers where this or a similar method has been used
Related Ontology Terms
References
See Help:References for how to manage references in OMPwiki.
- ↑ Anagnostopoulos, C & Spizizen, J (1961) REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS. J. Bacteriol. 81 741-6 PubMed OMPwiki page
