Minimal medium of Spizizen: Difference between revisions
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{{Pagetop}} | {{Pagetop}} | ||
== Purpose == | == Purpose == | ||
This medium can be used to prepare naturally competent cells of ''Bacillus subtilis''.<ref name='PMID:16561900'/> | |||
== Materials Needed == | == Materials Needed == | ||
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*1.4% dipotassium phosphate | *1.4% dipotassium phosphate | ||
*0.6% monopotassium phosphate | *0.6% monopotassium phosphate | ||
*0.1% sodium citrate | *0.1% sodium citrate (2H<sub>2</sub>O) | ||
*0.02% magnesium sulfate | *0.02% magnesium sulfate (7H<sub>2</sub>O) | ||
*0.5% glucose | *0.5% glucose | ||
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== Papers where this or a similar method has been used == | == Papers where this or a similar method has been used == | ||
<PMIDsummary /> | <PMIDsummary /> | ||
[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1212887/ PMID:4633157] | |||
== Related Ontology Terms == | == Related Ontology Terms == | ||
Latest revision as of 22:48, 23 June 2017
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Purpose
This medium can be used to prepare naturally competent cells of Bacillus subtilis.[1]
Materials Needed
Protocol
- 0.2% ammonium sulfate
- 1.4% dipotassium phosphate
- 0.6% monopotassium phosphate
- 0.1% sodium citrate (2H2O)
- 0.02% magnesium sulfate (7H2O)
- 0.5% glucose
Notes
Papers where this or a similar method has been used
<PMIDsummary /> PMID:4633157
Related Ontology Terms
References
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