PMID:19500142: Difference between revisions
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!align=left align='left' bgcolor='#CCCCFF' |Citation | |||
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'''Gu, G, Wang, N, Chaney, N, Smith, L and Lu, SE''' (2009) AmbR1 is a key transcriptional regulator for production of antifungal activity of Burkholderia contaminans strain MS14. ''FEMS Microbiol. Lett.'' '''297''':54-60 | |||
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!align=left align='left' bgcolor='#CCCCFF' |Abstract | |||
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Burkholderia contaminans strain MS14 has a broad range of antifungal activities to plant and human pathogens. In previous studies, a 22.7-kb genomic fragment harboring six genes was shown to be involved in the production of an antifungal oligopeptide in B. contaminans strain MS14. In this study, another LuxR-type regulatory gene, named ambR1, was identified downstream of the ambR2 gene, and three new ORFs were found upstream of ORF6 of the 22.7-kb fragment. Site-directed mutagenesis revealed that ambR1 was required for expression of the antifungal activity against the indicator fungus Geotrichum candidum. Transcription of all the putative genes (ORFs 2-9) identified in the region except ORF1 was regulated by both ambR1 and ambR2. The functional ambR1 gene was essential for transcription of ambR2, and constitutive expression of ambR2 did not restore the phenotype of the mutant MS14GG44(ambR1:nptII). Two of the three ORFs identified upstream from the ORF6 were predicted to encode two nonribosomal peptide synthetases (ORF7 and ORF9), and an insertion mutation in ORF9 resulted in the loss of antifungal activity against G. candidum. These results suggest that ambR1 is the key regulatory gene controlling the production of the antifungal activity of strain MS14. | |||
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!align=left align='left' bgcolor='#CCCCFF' |Links | |||
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=19500142 PubMed] | |||
Online version:[http://dx.doi.org/10.1111/j.1574-6968.2009.01653.x 10.1111/j.1574-6968.2009.01653.x] | |||
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!align=left align='left' bgcolor='#CCCCFF' |Keywords | |||
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Antifungal Agents/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Burkholderia/genetics; Burkholderia/metabolism; Gene Expression Regulation, Bacterial; Genes, Regulator; Transcription, Genetic | |||
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==Main Points of the Paper == | |||
{{LitSignificance}} | |||
== Materials and Methods Used == | |||
{{LitMaterials}} | |||
==Phenotype Annotations== | |||
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="P557b190d9715f" class=" tableEdit Phenotype_Table_2" | |||
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==Notes== | |||
==References== | |||
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[[Category:Publication]] | |||
Latest revision as of 17:38, 12 June 2015
| Citation |
Gu, G, Wang, N, Chaney, N, Smith, L and Lu, SE (2009) AmbR1 is a key transcriptional regulator for production of antifungal activity of Burkholderia contaminans strain MS14. FEMS Microbiol. Lett. 297:54-60 |
|---|---|
| Abstract |
Burkholderia contaminans strain MS14 has a broad range of antifungal activities to plant and human pathogens. In previous studies, a 22.7-kb genomic fragment harboring six genes was shown to be involved in the production of an antifungal oligopeptide in B. contaminans strain MS14. In this study, another LuxR-type regulatory gene, named ambR1, was identified downstream of the ambR2 gene, and three new ORFs were found upstream of ORF6 of the 22.7-kb fragment. Site-directed mutagenesis revealed that ambR1 was required for expression of the antifungal activity against the indicator fungus Geotrichum candidum. Transcription of all the putative genes (ORFs 2-9) identified in the region except ORF1 was regulated by both ambR1 and ambR2. The functional ambR1 gene was essential for transcription of ambR2, and constitutive expression of ambR2 did not restore the phenotype of the mutant MS14GG44(ambR1:nptII). Two of the three ORFs identified upstream from the ORF6 were predicted to encode two nonribosomal peptide synthetases (ORF7 and ORF9), and an insertion mutation in ORF9 resulted in the loss of antifungal activity against G. candidum. These results suggest that ambR1 is the key regulatory gene controlling the production of the antifungal activity of strain MS14. |
| Links |
PubMed Online version:10.1111/j.1574-6968.2009.01653.x |
| Keywords |
Antifungal Agents/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Burkholderia/genetics; Burkholderia/metabolism; Gene Expression Regulation, Bacterial; Genes, Regulator; Transcription, Genetic |
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Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
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Notes
References
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