Difference between revisions of "PMID:8820655"

From OMPwiki
(Table edited by Tkveton via TableEdit)
(Table edited by Tkveton via TableEdit)
 
(8 intermediate revisions by the same user not shown)
Line 60: Line 60:
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
|
 
|
−
*Genotype of Reference Strain: ''recF''
+
*Genotype of Reference Strain: ''recF+''
 
*Genotype of Experimental Strain : ''recF349''
 
*Genotype of Experimental Strain : ''recF349''
 
|
 
|
−
*Reference Condition:
+
*Reference Condition: 56/2 minimal medium at 37C
 
|
 
|
  
 
|
 
|
  
 +
|
 +
ECO:0001027
 +
|
 +
colony counting evidence
 +
|
 +
Mutations show approximately 50% decrease in viability relative to the wild type; Reference Table 1
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: DM4000 recF missense
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''recF+''
 +
*Genotype of Experimental Strain : ''recF missense''
 +
|
 +
*Reference Condition: 56/2 minimal medium at 37C
 +
|
 +
 +
|
 +
 +
|
 +
ECO:0001027
 +
|
 +
colony counting evidence
 +
|
 +
No decrease in cell viability;
 +
Reference Table 1
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: DM4000 priA2::kan recF349
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''priA+ recF+''
 +
*Genotype of Experimental Strain : ''priA2::kan recF349''
 +
|
 +
*Reference Condition: 56/2 minimal medium at 37C
 
|
 
|
  
Line 73: Line 120:
  
 
|
 
|
−
Mutations show approximately 50% decrease in viability relative to the wild type, table 1.
+
ECO:0000052
 +
|
 +
suppressor/enhancer interaction evidence
 +
|
 +
abolished cell viability, this, along with both single mutants being able to transduce with the wild type, leads to conclusion that remaining viability in priA2::kan strain due to recF, and remaining viability in redF349 strain due to priA
 
|
 
|
 
   
 
   
Line 82: Line 133:
 
*Taxon: Escherichia coli
 
*Taxon: Escherichia coli
 
*Strain: K-12
 
*Strain: K-12
−
*Substrain: JC18991
+
*Substrain: JC18983
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
|
 
|
−
*Genotype of Reference Strain: ''recF'' ''priA''
+
*Genotype of Reference Strain: ''priA+''
−
*Genotype of Experimental Strain : recF4101 priA2:kan
+
*Genotype of Experimental Strain : ''priA2::kan''
 
|
 
|
−
*Reference Condition:
+
*Reference Condition: 56/2 minimal medium at 37C
 
|
 
|
  
 
|
 
|
  
 +
|
 +
ECO:0001027
 +
|
 +
colony counting evidence
 +
|
 +
Ten-fold decrease in cell viability;
 +
Reference Table 1
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: JC18989
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''priA+ recF+''
 +
*Genotype of Experimental Strain : ''priA2::kan recF143''
 +
|
 +
*Reference Condition: 56/2 minimal medium at 37C
 
|
 
|
  
Line 98: Line 171:
  
 
|
 
|
−
Double mutation decreases cell viability from wildtype, table 1.
+
ECO:0001027
 +
|
 +
colony counting evidence
 +
|
 +
decreased cell viability twofold compared to wild type; Reference Table 1
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: JC18991
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''priA+ recF+''
 +
*Genotype of Experimental Strain : ''priA2::kan recF4101''
 +
|
 +
*Reference Condition: 56/2 minimal medium at 37C
 +
|
 +
 
 +
|
 +
 
 +
|
 +
ECO:0001027
 +
|
 +
colony counting evidence
 +
|
 +
decreased cell viability fivefold compared to wild type; Reference Table 1
 
|
 
|
 
   
 
   
Line 110: Line 212:
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
|
 
|
−
*Genotype of Reference Strain: ''recA'' ''priA''
+
*Genotype of Reference Strain: ''priA+ recF+''
−
*Genotype of Experimental Strain : ''recA4115'' ''priA2::kan''
+
*Genotype of Experimental Strain : ''priA2::kan recF4115''
 
|
 
|
−
*Reference Condition:
+
*Reference Condition: 56/2 minimal medium at 37C
 
|
 
|
  
 
|
 
|
  
 +
|
 +
ECO:0001027
 +
|
 +
colony counting evidence
 +
|
 +
decreased cell viability fivefold compared to wild type; Reference Table 1
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: JC19028
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''priA+ recO++''
 +
*Genotype of Experimental Strain : ''priA2::kan recO1504::Tn5''
 +
|
 +
*Reference Condition: 56/2 minimal medium at 37C
 
|
 
|
  
Line 123: Line 246:
  
 
|
 
|
−
Double mutation decreases cell viability, table 1.
+
ECO:0001027
 +
|
 +
colony counting evidence
 +
|
 +
threefold increase in cell viability; Reference Table 1
 
|
 
|
 
   
 
   
Line 132: Line 259:
 
*Taxon: Escherichia coli
 
*Taxon: Escherichia coli
 
*Strain: K-12
 
*Strain: K-12
−
*Substrain: JC18989
+
*Substrain: JC19029
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
|
 
|
−
*Genotype of Reference Strain: ''recF'' ''priA''
+
*Genotype of Reference Strain: ''priA+ recR+''
−
*Genotype of Experimental Strain : ''recF143'' ''priA2::kan''
+
*Genotype of Experimental Strain : ''priA2::kan recR252::Tn10-9''
 
|
 
|
−
*Reference Condition:
+
*Reference Condition: 56/2 minimal medium at 37C
 
|
 
|
  
 
|
 
|
  
 +
|
 +
ECO:0001027
 +
|
 +
colony counting evidence
 +
|
 +
slightly increased cell viability; Reference Table 1
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: JC19002
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''priA+ recF+ dnaC+''
 +
*Genotype of Experimental Strain : ''priA2::kan recF143 dnaC809''
 +
|
 +
*Reference Condition: 56/2 minimal medium at 37C
 
|
 
|
  
Line 148: Line 296:
  
 
|
 
|
−
Double mutation causes decreased cell viability, table 1.
+
ECO:0001027
 +
|
 +
colony counting evidence
 +
|
 +
restored cell viability to that of the wild type; Reference Table 1
 
|
 
|
 
   
 
   
Line 157: Line 309:
 
*Taxon: Escherichia coli
 
*Taxon: Escherichia coli
 
*Strain: K-12
 
*Strain: K-12
−
*Substrain: DM4000
+
*Substrain: JC19111
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
|
 
|
−
*Genotype of Reference Strain: ''recF''
+
*Genotype of Reference Strain: ''priA+ recF+ dnaC+''
−
*Genotype of Experimental Strain : ''recF349''
+
*Genotype of Experimental Strain : ''priA2::kan recF349 dnaC809''
 
|
 
|
−
*Reference Condition:
+
*Reference Condition: 56/2 minimal medium at 37C
 
|
 
|
  
Line 173: Line 325:
 
colony counting evidence
 
colony counting evidence
 
|
 
|
−
Mutations show approximately 50% decrease in viability relative to the wild type, table 1.
+
restored cell viability to that of the wild type; Reference Table 1
 
|
 
|
 
   
 
   
Line 182: Line 334:
 
*Taxon: Escherichia coli
 
*Taxon: Escherichia coli
 
*Strain: K-12
 
*Strain: K-12
−
*Substrain: DM4000
+
*Substrain: JC19109
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
|
 
|
−
*Genotype of Reference Strain: recF+
+
*Genotype of Reference Strain: ''priA+ recF+ dnaC+''
−
*Genotype of Experimental Strain : recF missense
+
*Genotype of Experimental Strain : ''priA2::kan recF4101 dnaC809''
 
|
 
|
−
*Reference Condition:
+
*Reference Condition: 56/2 minimal medium at 37C
 
|
 
|
  
Line 198: Line 350:
 
colony counting evidence
 
colony counting evidence
 
|
 
|
−
No decrease in cell viability;
+
restored cell viability to that of the wild type; Reference Table 1
−
Reference Table 1
+
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: JC18989
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''priA2::kan recF+''
 +
*Genotype of Experimental Strain : ''priA2::kan recF143''
 +
|
 +
*Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds
 +
|
 +
OMP:0005021
 +
|
 +
decreased basal expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Decreased relative to priA2::kan single mutant; Reference Figure 1
 
|
 
|
 
   
 
   
Line 208: Line 384:
 
*Taxon: Escherichia coli
 
*Taxon: Escherichia coli
 
*Strain: K-12
 
*Strain: K-12
−
*Substrain: DM4000
+
*Substrain: JC19110
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
|
 
|
−
*Genotype of Reference Strain: priA+
+
*Genotype of Reference Strain: ''priA+ recF+ dnaC+''
−
*Genotype of Experimental Strain : priA2::kan
+
*Genotype of Experimental Strain : ''priA2::kan recF4115 dnaC809''
 
|
 
|
−
*Reference Condition:
+
*Reference Condition: 56/2 minimal medium at 37C
 
|
 
|
  
Line 224: Line 400:
 
colony counting evidence
 
colony counting evidence
 
|
 
|
−
Ten-fold decrease in cell viability;  
+
restored cell viability to that of the wild type; Reference Table 1
−
Reference Table 1
+
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: JC18989
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''priA+ recF143''
 +
*Genotype of Experimental Strain : ''priA2::kan recF143''
 +
|
 +
*Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds
 +
|
 +
OMP:0005020
 +
|
 +
increased basal expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Increased relative to recF143 single mutant; Reference Figure 1
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: JC19002
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''priA2::kan recF143 dnaC+''
 +
*Genotype of Experimental Strain : ''priAS::kan recF143 dnaC809''
 +
|
 +
*Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds
 +
|
 +
OMP:0005021
 +
|
 +
decreased basal expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Decreased relative to priA2::kan recF143 double mutant; Restored basal SOS expression to that of the recF143 mutant strain; Reference Figure 1
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: JC19002
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''priA+ recF+ dnaC+''
 +
*Genotype of Experimental Strain : ''priAS::kan recF143 dnaC809''
 +
|
 +
*Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds
 +
|
 +
OMP:0005025
 +
|
 +
decreased induced expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Decreased relative to wild type, stayed the same relative to priA2::kan recF143 double mutant; Reference Figure 1
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: JC18989
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''recO+''
 +
*Genotype of Experimental Strain : ''recO1504::Tn5''
 +
|
 +
*Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds
 +
|
 +
OMP:0005020
 +
|
 +
increased basal expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Increased relative to wildtype; Reference Figure 2
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: JC18989
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''recR+''
 +
*Genotype of Experimental Strain : ''recR252::Tn10-9''
 +
|
 +
*Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds
 +
|
 +
OMP:0005020
 +
|
 +
increased basal expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Increased relative to wildtype; Reference Figure 2
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: JC18989
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''recO1504::Tn5 priA+''
 +
*Genotype of Experimental Strain : ''recO1504::Tn5 priA2::kan''
 +
|
 +
*Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds
 +
|
 +
OMP:0005020
 +
|
 +
increased basal expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Increased relative to recO single mutant; Reference Figure 2
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: JC18989
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''recR252::Tn10-9 priA+''
 +
*Genotype of Experimental Strain : ''recR252::Tn10-9 priA2::kan''
 +
|
 +
*Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds
 +
|
 +
OMP:0005020
 +
|
 +
increased basal expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Increased relative to recR single mutant; Reference Figure 2
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: JC18989
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''recO+''
 +
*Genotype of Experimental Strain : ''recO1504::Tn5''
 +
|
 +
*Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds
 +
|
 +
OMP:0005025
 +
|
 +
decreased induced expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Decreased relative to wildtype; Reference Figure 2
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: JC18989
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''recR+''
 +
*Genotype of Experimental Strain : ''recR252::Tn10-9''
 +
|
 +
*Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds
 +
|
 +
OMP:0005025
 +
|
 +
decreased induced expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Decreased relative to wildtype; Reference Figure 2
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: JC18989
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''recO1504::Tn5 priA+''
 +
*Genotype of Experimental Strain : ''recO1504::Tn5 priA2::kan''
 +
|
 +
*Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds
 +
|
 +
OMP:0005023
 +
|
 +
presence of an SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Stayed constant compared to recO single mutant; Reference Figure 2
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: JC18989
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''recR252::Tn10-9 priA+''
 +
*Genotype of Experimental Strain : ''recR252::Tn10-9 priA2::kan''
 +
|
 +
*Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds
 +
|
 +
OMP:0005025
 +
|
 +
decreased induced expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Decreased relative to recR single mutant; Reference Figure 2
 
|
 
|
 
   
 
   

Latest revision as of 14:44, 3 June 2015

Citation

Sandler, SJ (1996) Overlapping functions for recF and priA in cell viability and UV-inducible SOS expression are distinguished by dnaC809 in Escherichia coli K-12.Mol. Microbiol. 19:871-80

Abstract

The recF and priA genes have roles in DNA repair and homologous recombination. Mutations in these genes also cause decreases in cell viability and alterations in UV-inducible sulAp-lacZ (SOS) expression. To find out if the two genes are in the same or different pathways for viability and SOS expression, the phenotypes of the double mutant strains were studied. The recF priA double mutant showed a lower viability and SOS expression level than either of the single mutants. In the case of cell viability, recF missense mutations decreased viability of a priA2::kan strain two to five-fold whereas recF null priA2::kan double mutants were not viable at all. dnaC809, a mutation that suppresses the UV-sensitive (UVs and Rec- phenotypes of priA2::kan, restored cell viability, but not UV-inducible SOS expression, to a priA recF strain. Since recF is epistatic with recO and recR (recOR) for UV resistance, recOR mutations were also tested with priA2::kan. No overlap was found between recOR and priA for viability and SOS expression. It is concluded that priA and recF have two different overlapping functions in viability and SOS expression that are distinguishable by the effects of dnaC809. The role of recF in a priA2::kan strain in cell viability is a new function for recF and unlike recF's other roles in DNA repair and recombination, is independent of recOR. A new role for priA in UV-inducible SOS expression in a recF mutant is also defined.

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Keywords

Bacterial Proteins; DNA Repair; DNA, Bacterial; DNA-Binding Proteins; Escherichia coli; Escherichia coli Proteins; Genes, Bacterial; Lac Operon; Mutation; Recombinant Fusion Proteins; Recombination, Genetic; Replication Protein A; SOS Response (Genetics); Ultraviolet Rays

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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC18536
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: recF+
  • Genotype of Experimental Strain : recF349
  • Reference Condition: 56/2 minimal medium at 37C
 ECO:0001027

colony counting evidence

Mutations show approximately 50% decrease in viability relative to the wild type; Reference Table 1

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: DM4000 recF missense
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: recF+
  • Genotype of Experimental Strain : recF missense
  • Reference Condition: 56/2 minimal medium at 37C
 ECO:0001027

colony counting evidence

No decrease in cell viability; Reference Table 1

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: DM4000 priA2::kan recF349
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: priA+ recF+
  • Genotype of Experimental Strain : priA2::kan recF349
  • Reference Condition: 56/2 minimal medium at 37C
 ECO:0000052

suppressor/enhancer interaction evidence

abolished cell viability, this, along with both single mutants being able to transduce with the wild type, leads to conclusion that remaining viability in priA2::kan strain due to recF, and remaining viability in redF349 strain due to priA

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC18983
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: priA+
  • Genotype of Experimental Strain : priA2::kan
  • Reference Condition: 56/2 minimal medium at 37C
 ECO:0001027

colony counting evidence

Ten-fold decrease in cell viability; Reference Table 1

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC18989
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: priA+ recF+
  • Genotype of Experimental Strain : priA2::kan recF143
  • Reference Condition: 56/2 minimal medium at 37C
 ECO:0001027

colony counting evidence

decreased cell viability twofold compared to wild type; Reference Table 1

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC18991
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: priA+ recF+
  • Genotype of Experimental Strain : priA2::kan recF4101
  • Reference Condition: 56/2 minimal medium at 37C
 ECO:0001027

colony counting evidence

decreased cell viability fivefold compared to wild type; Reference Table 1

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC18996
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: priA+ recF+
  • Genotype of Experimental Strain : priA2::kan recF4115
  • Reference Condition: 56/2 minimal medium at 37C
 ECO:0001027

colony counting evidence

decreased cell viability fivefold compared to wild type; Reference Table 1

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC19028
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: priA+ recO++
  • Genotype of Experimental Strain : priA2::kan recO1504::Tn5
  • Reference Condition: 56/2 minimal medium at 37C
 ECO:0001027

colony counting evidence

threefold increase in cell viability; Reference Table 1

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC19029
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: priA+ recR+
  • Genotype of Experimental Strain : priA2::kan recR252::Tn10-9
  • Reference Condition: 56/2 minimal medium at 37C
 ECO:0001027

colony counting evidence

slightly increased cell viability; Reference Table 1

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC19002
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: priA+ recF+ dnaC+
  • Genotype of Experimental Strain : priA2::kan recF143 dnaC809
  • Reference Condition: 56/2 minimal medium at 37C
 ECO:0001027

colony counting evidence

restored cell viability to that of the wild type; Reference Table 1

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC19111
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: priA+ recF+ dnaC+
  • Genotype of Experimental Strain : priA2::kan recF349 dnaC809
  • Reference Condition: 56/2 minimal medium at 37C
 ECO:0001027

colony counting evidence

restored cell viability to that of the wild type; Reference Table 1

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC19109
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: priA+ recF+ dnaC+
  • Genotype of Experimental Strain : priA2::kan recF4101 dnaC809
  • Reference Condition: 56/2 minimal medium at 37C
 ECO:0001027

colony counting evidence

restored cell viability to that of the wild type; Reference Table 1

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC18989
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: priA2::kan recF+
  • Genotype of Experimental Strain : priA2::kan recF143
  • Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds
 OMP:0005021

decreased basal expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Decreased relative to priA2::kan single mutant; Reference Figure 1

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC19110
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: priA+ recF+ dnaC+
  • Genotype of Experimental Strain : priA2::kan recF4115 dnaC809
  • Reference Condition: 56/2 minimal medium at 37C
 ECO:0001027

colony counting evidence

restored cell viability to that of the wild type; Reference Table 1

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC18989
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: priA+ recF143
  • Genotype of Experimental Strain : priA2::kan recF143
  • Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds
 OMP:0005020

increased basal expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Increased relative to recF143 single mutant; Reference Figure 1

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC19002
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: priA2::kan recF143 dnaC+
  • Genotype of Experimental Strain : priAS::kan recF143 dnaC809
  • Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds
 OMP:0005021

decreased basal expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Decreased relative to priA2::kan recF143 double mutant; Restored basal SOS expression to that of the recF143 mutant strain; Reference Figure 1

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC19002
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: priA+ recF+ dnaC+
  • Genotype of Experimental Strain : priAS::kan recF143 dnaC809
  • Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds
 OMP:0005025

decreased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Decreased relative to wild type, stayed the same relative to priA2::kan recF143 double mutant; Reference Figure 1

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC18989
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: recO+
  • Genotype of Experimental Strain : recO1504::Tn5
  • Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds
 OMP:0005020

increased basal expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Increased relative to wildtype; Reference Figure 2

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC18989
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: recR+
  • Genotype of Experimental Strain : recR252::Tn10-9
  • Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds
 OMP:0005020

increased basal expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Increased relative to wildtype; Reference Figure 2

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC18989
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: recO1504::Tn5 priA+
  • Genotype of Experimental Strain : recO1504::Tn5 priA2::kan
  • Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds
 OMP:0005020

increased basal expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Increased relative to recO single mutant; Reference Figure 2

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC18989
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: recR252::Tn10-9 priA+
  • Genotype of Experimental Strain : recR252::Tn10-9 priA2::kan
  • Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds
 OMP:0005020

increased basal expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Increased relative to recR single mutant; Reference Figure 2

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC18989
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: recO+
  • Genotype of Experimental Strain : recO1504::Tn5
  • Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds
 OMP:0005025

decreased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Decreased relative to wildtype; Reference Figure 2

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC18989
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: recR+
  • Genotype of Experimental Strain : recR252::Tn10-9
  • Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds
 OMP:0005025

decreased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Decreased relative to wildtype; Reference Figure 2

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC18989
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: recO1504::Tn5 priA+
  • Genotype of Experimental Strain : recO1504::Tn5 priA2::kan
  • Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds
 OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Stayed constant compared to recO single mutant; Reference Figure 2

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: JC18989
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: recR252::Tn10-9 priA+
  • Genotype of Experimental Strain : recR252::Tn10-9 priA2::kan
  • Reference Condition: minimal medium at 37C; 0.5 J/m^2 s for 5 seconds
 OMP:0005025

decreased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Decreased relative to recR single mutant; Reference Figure 2


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