PMID:4599365: Difference between revisions
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==Main Points of the Paper == | ==Main Points of the Paper == | ||
*Hfr strains suppress initiation of DNA replication mutants via integrative suppression, giving rise to temperature-resistant revertants | |||
*The presence of other plasmids affect the reversion rate in Hfr strains | |||
**Multiple excisions and integrations may take place- the presence of other plasmids may be blocking proper sites for F factor. | |||
*F-like but not I-like plasmids support integrative suppression in ''dnaA'' strains defective for initiation of DNA replication, not ''dnaC'' mutants | |||
*Non-transmissible ColE1 and ColE2 plasmids do not cause integrative suppression | |||
*Most of the time the entire plasmid is integrated into the chromosome of the suppressed revertants | |||
**Hemolytic factor integrates only part of the plasmid while the other part remains autonomous | |||
*Autonomous ColE1 plasmid can only be maintained in limited copies when the chromosome is being replicated under integrative suppression | |||
*Autonomous R1 factor replication continues under integrative suppression | |||
== Materials and Methods Used == | == Materials and Methods Used == | ||
*Integrative | *[[:Category:Integrative Suppression|Integrative Suppression]] | ||
*EtBr sensitivity assay | *EtBr sensitivity assay | ||
*Monitor growth rate | *Monitor growth rate | ||
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''Escherichia coli'' | ''Escherichia coli'' | ||
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Hfr-120/6 | Hfr-120/6 | ||
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thiamine auxotroph | |||
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Growth | |||
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Plating Assay | |||
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thymine auxotroph | |||
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Growth | Growth | ||
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PC2 | |||
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leucine auxotroph | |||
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Growth | Growth | ||
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streptomyin resistant | |||
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''Escherichia coli'' | ''Escherichia coli'' | ||
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NCBI: | |||
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Hfr-120/6 | |||
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temperature sensitive defect in initiation of DNA replication | |||
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<references/> | <references/> | ||
[[Category: | [[Category:Integrative Suppression]] | ||
[[Category:Publication]] | |||
[[Category:To Be Converted]] | |||
Latest revision as of 17:09, 22 June 2011
| Citation |
Goebel, W (1974) Integrative suppression of temperature-sensitive mutants iwth a lesion in the initiation of DNA replication. Replication of autonomous plasmids in the suppressed state.Eur. J. Biochem. 43:125-30 |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Centrifugation, Density Gradient; Chromosome Mapping; DNA Replication; Drug Resistance, Microbial; Escherichia coli; Extrachromosomal Inheritance; Gene Frequency; Mutation; Species Specificity; Streptomycin; Suppression, Genetic; Temperature; Thymine; Tritium |
| edit table |
Main Points of the Paper
- Hfr strains suppress initiation of DNA replication mutants via integrative suppression, giving rise to temperature-resistant revertants
- The presence of other plasmids affect the reversion rate in Hfr strains
- Multiple excisions and integrations may take place- the presence of other plasmids may be blocking proper sites for F factor.
- F-like but not I-like plasmids support integrative suppression in dnaA strains defective for initiation of DNA replication, not dnaC mutants
- Non-transmissible ColE1 and ColE2 plasmids do not cause integrative suppression
- Most of the time the entire plasmid is integrated into the chromosome of the suppressed revertants
- Hemolytic factor integrates only part of the plasmid while the other part remains autonomous
- Autonomous ColE1 plasmid can only be maintained in limited copies when the chromosome is being replicated under integrative suppression
- Autonomous R1 factor replication continues under integrative suppression
Materials and Methods Used
- Integrative Suppression
- EtBr sensitivity assay
- Monitor growth rate
- Thymidine incorporation to measure DNA synthesis rate
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
|
CRT-46 |
streptomycin resistant |
Resistance to |
Plating Assay |
|||||
|
CRT-46 |
abolished lactose utilization |
Metabolic Activity |
Plating Assay |
|||||
|
CRT-46 |
threonine auxotroph |
Growth |
Plating Assay |
|||||
|
CRT-46 |
abolished lactose utilization |
Metabolic Activity |
Plating Assay |
|||||
|
CRT-46 |
leucine auxotroph |
Growth |
Plating Assay |
|||||
|
CRT-46 |
isoleucine auxotroph |
Growth |
Plating Assay |
|||||
|
CRT-46 |
thiamine auxotroph |
Growth |
Plating Assay |
|||||
|
CRT-46 |
thymine auxotroph |
Growth |
Plating Assay |
|||||
|
Escherichia coli |
Hfr-120/6 |
thiamine auxotroph |
Growth |
Plating Assay |
||||
|
Escherichia coli |
Hfr-120/6 |
thymine auxotroph |
Growth |
Plating Assay |
||||
|
Escherichia coli |
PC2 |
thymine auxotroph |
Growth |
Plating Assay |
||||
|
Escherichia coli |
PC2 |
leucine auxotroph |
Growth |
Plating Assay |
||||
|
Escherichia coli |
PC2 |
streptomyin resistant |
||||||
|
Escherichia coli |
NCBI: |
Hfr-120/6 |
temperature sensitive defect in initiation of DNA replication |
| ||||
| edit table |
Notes
References
See Help:References for how to manage references in OMPwiki.
