PMID:4599365

Citation	Goebel, W (1974) Integrative suppression of temperature-sensitive mutants iwth a lesion in the initiation of DNA replication. Replication of autonomous plasmids in the suppressed state.Eur. J. Biochem. 43:125-30
Abstract	No abstract in PubMed
Links	PubMed
Keywords	Centrifugation, Density Gradient; Chromosome Mapping; DNA Replication; Drug Resistance, Microbial; Escherichia coli; Extrachromosomal Inheritance; Gene Frequency; Mutation; Species Specificity; Streptomycin; Suppression, Genetic; Temperature; Thymine; Tritium
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Main Points of the Paper

• Hfr strains suppress initiation of DNA replication mutants via integrative suppression, giving rise to temperature-resistant revertants
• The presence of other plasmids affect the reversion rate in Hfr strains
  • Multiple excisions and integrations may take place- the presence of other plasmids may be blocking proper sites for F factor.
• F-like but not I-like plasmids support integrative suppression in dnaA strains defective for initiation of DNA replication, not dnaC mutants
• Non-transmissible ColE1 and ColE2 plasmids do not cause integrative suppression
• Most of the time the entire plasmid is integrated into the chromosome of the suppressed revertants
  • Hemolytic factor integrates only part of the plasmid while the other part remains autonomous
• Autonomous ColE1 plasmid can only be maintained in limited copies when the chromosome is being replicated under integrative suppression
• Autonomous R1 factor replication continues under integrative suppression

Materials and Methods Used

• Integrative Suppression
• EtBr sensitivity assay
• Monitor growth rate
• Thymidine incorporation to measure DNA synthesis rate

Phenotype Annotations

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Species	Taxon ID	Strain	Gene (if known)	OMP	Phenotype	Details	Evidence	Notes
		CRT-46		streptomycin resistant	Resistance to		Plating Assay
		CRT-46		abolished lactose utilization	Metabolic Activity		Plating Assay
		CRT-46		threonine auxotroph	Growth		Plating Assay
		CRT-46		abolished lactose utilization	Metabolic Activity		Plating Assay
		CRT-46		leucine auxotroph	Growth		Plating Assay
		CRT-46		isoleucine auxotroph	Growth		Plating Assay
		CRT-46		thiamine auxotroph	Growth		Plating Assay
		CRT-46		thymine auxotroph	Growth		Plating Assay
Escherichia coli		Hfr-120/6		thiamine auxotroph	Growth		Plating Assay
Escherichia coli		Hfr-120/6		thymine auxotroph	Growth		Plating Assay
Escherichia coli		PC2		thymine auxotroph	Growth		Plating Assay
Escherichia coli		PC2		leucine auxotroph	Growth		Plating Assay
Escherichia coli		PC2		streptomyin resistant
Escherichia coli	NCBI:	Hfr-120/6		temperature sensitive defect in initiation of DNA replication
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References

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