PMID:4599365: Difference between revisions

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!align=left  |Citation
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'''Goebel, W'''  (1974) Integrative suppression of temperature-sensitive mutants iwth a lesion in the initiation of DNA replication. Replication of autonomous plasmids in the suppressed state.''Eur. J. Biochem.'' '''43''':125-30
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!align=left  |Abstract
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No abstract in PubMed
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!align=left  |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4599365 PubMed]
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!align=left  |Keywords
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Centrifugation, Density Gradient; Chromosome Mapping; DNA Replication; Drug Resistance, Microbial; Escherichia coli; Extrachromosomal Inheritance; Gene Frequency; Mutation; Species Specificity; Streptomycin; Suppression, Genetic; Temperature; Thymine; Tritium
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==Main Points of the Paper ==
*Hfr strains suppress initiation of DNA replication mutants via integrative suppression, giving rise to temperature-resistant revertants
*The presence of other plasmids affect the reversion rate in Hfr strains
**Multiple excisions and integrations may take place- the presence of other plasmids may be blocking proper sites for F factor.
*F-like but not I-like plasmids support integrative suppression in ''dnaA'' strains defective for initiation of DNA replication, not ''dnaC'' mutants
*Non-transmissible ColE1 and ColE2 plasmids do not cause integrative suppression
*Most of the time the entire plasmid is integrated into the chromosome of the suppressed revertants
**Hemolytic factor integrates only part of the plasmid while the other part remains autonomous
*Autonomous ColE1 plasmid can only be maintained in limited copies when the chromosome is being replicated under integrative suppression
*Autonomous R1 factor replication continues under integrative suppression
== Materials and Methods Used ==
*[[:Category:Integrative Suppression|Integrative Suppression]]
*EtBr sensitivity assay
*Monitor growth rate
*Thymidine incorporation to measure DNA synthesis rate
==Phenotype Annotations==
{{AnnotationTableHelp}}
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{|  id="G4d76b1da7a7b1"  class=" tableEdit PMID_Phenotype_table" 
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!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
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CRT-46
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streptomycin resistant
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Resistance to
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|
Plating Assay
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|-
|
|
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CRT-46
|
|
abolished lactose utilization
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Metabolic Activity
|
|
Plating Assay
|
|-
|
|
|
CRT-46
|
|
threonine auxotroph
|
Growth
|
|
Plating Assay
|
|-
|
|
|
CRT-46
|
|
abolished lactose utilization
|
Metabolic Activity
|
|
Plating Assay
|
|-
|
|
|
CRT-46
|
|
leucine auxotroph
|
Growth
|
|
Plating Assay
|
|-
|
|
|
CRT-46
|
|
isoleucine auxotroph
|
Growth
|
|
Plating Assay
|
|-
|
|
|
CRT-46
|
|
thiamine auxotroph
|
Growth
|
|
Plating Assay
|
|-
|
|
|
CRT-46
|
|
thymine auxotroph
|
Growth
|
|
Plating Assay
|
|-
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''Escherichia coli''
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Hfr-120/6
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thiamine auxotroph
|
Growth
|
|
Plating Assay
|
|-
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''Escherichia coli''
|
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Hfr-120/6
|
|
thymine auxotroph
|
Growth
|
|
Plating Assay
|
|-
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''Escherichia coli''
|
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PC2
|
|
thymine auxotroph
|
Growth
|
|
Plating Assay
|
|-
|
''Escherichia coli''
|
|
PC2
|
|
leucine auxotroph
|
Growth
|
|
Plating Assay
|
|-
|
''Escherichia coli''
|
|
PC2
|
|
streptomyin resistant
|
|
|
|
|-
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''Escherichia coli''
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NCBI:
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Hfr-120/6
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temperature sensitive defect in initiation of DNA replication
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|
|
|
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==Notes==
==References==
{{RefHelp}}
<references/>
[[Category:Integrative Suppression]]
[[Category:Publication]]
[[Category:To Be Converted]]

Latest revision as of 17:09, 22 June 2011

Citation

Goebel, W (1974) Integrative suppression of temperature-sensitive mutants iwth a lesion in the initiation of DNA replication. Replication of autonomous plasmids in the suppressed state.Eur. J. Biochem. 43:125-30

Abstract

No abstract in PubMed

Links

PubMed

Keywords

Centrifugation, Density Gradient; Chromosome Mapping; DNA Replication; Drug Resistance, Microbial; Escherichia coli; Extrachromosomal Inheritance; Gene Frequency; Mutation; Species Specificity; Streptomycin; Suppression, Genetic; Temperature; Thymine; Tritium

edit table

Main Points of the Paper

  • Hfr strains suppress initiation of DNA replication mutants via integrative suppression, giving rise to temperature-resistant revertants
  • The presence of other plasmids affect the reversion rate in Hfr strains
    • Multiple excisions and integrations may take place- the presence of other plasmids may be blocking proper sites for F factor.
  • F-like but not I-like plasmids support integrative suppression in dnaA strains defective for initiation of DNA replication, not dnaC mutants
  • Non-transmissible ColE1 and ColE2 plasmids do not cause integrative suppression
  • Most of the time the entire plasmid is integrated into the chromosome of the suppressed revertants
    • Hemolytic factor integrates only part of the plasmid while the other part remains autonomous
  • Autonomous ColE1 plasmid can only be maintained in limited copies when the chromosome is being replicated under integrative suppression
  • Autonomous R1 factor replication continues under integrative suppression

Materials and Methods Used

  • Integrative Suppression
  • EtBr sensitivity assay
  • Monitor growth rate
  • Thymidine incorporation to measure DNA synthesis rate

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.

Species Taxon ID Strain Gene (if known) OMP Phenotype Details Evidence Notes

CRT-46

streptomycin resistant

Resistance to

Plating Assay

CRT-46

abolished lactose utilization

Metabolic Activity

Plating Assay

CRT-46

threonine auxotroph

Growth

Plating Assay

CRT-46

abolished lactose utilization

Metabolic Activity

Plating Assay

CRT-46

leucine auxotroph

Growth

Plating Assay

CRT-46

isoleucine auxotroph

Growth

Plating Assay

CRT-46

thiamine auxotroph

Growth

Plating Assay

CRT-46

thymine auxotroph

Growth

Plating Assay

Escherichia coli

Hfr-120/6

thiamine auxotroph

Growth

Plating Assay

Escherichia coli

Hfr-120/6

thymine auxotroph

Growth

Plating Assay

Escherichia coli

PC2

thymine auxotroph

Growth

Plating Assay

Escherichia coli

PC2

leucine auxotroph

Growth

Plating Assay

Escherichia coli

PC2

streptomyin resistant

Escherichia coli

NCBI:

Hfr-120/6

temperature sensitive defect in initiation of DNA replication


edit table


Notes

References

See Help:References for how to manage references in OMPwiki.

Retrieved from "https://microbialphenotypes.org/wiki/index.php?title=PMID:4599365&oldid=5207"