PMID:4927942: Difference between revisions

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!align=left  |Citation
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'''Nishimura, Y, Caro, L, Berg, CM and Hirota, Y'''  (1971) Chromosome replication in Escherichia coli. IV. Control of chromosome replication and cell division by an integrated episome.''J. Mol. Biol.'' '''55''':441-56
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!align=left  |Abstract
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No abstract in PubMed
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!align=left  |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4927942 PubMed]
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!align=left  |Keywords
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Integrative Suppression; Hfr; Phage; Acridine Orange; Ethidium Bromide; DNA Replication; Reversion; Temperature-sensitivity
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==Main Points of the Paper ==
*New type of suppression of a temperature-sensitive mutation of DNA replication- the chromosome may be incorporated into the F replicon in Hfr strains
== Materials and Methods Used ==
*Acridine orange sensitivity assay
*EtBr sensitivity assay
*Cross-streak test for conjugation
*Transduction
==Phenotype Annotations==
{{AnnotationTableHelp}}
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!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
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''Escherichia coli''
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NCBI:83333
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CRT46
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*slow stop DNA replication defect
*abolished initiation of DNA replication at high temperature
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Biochemical Assay
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''Escherichia coli''
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NCBI:83333
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CRT226
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*fast stop DNA replication defect
*abolished DNA replication at high temperature
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|
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Biochemical Assay
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''Escherichia coli''
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NCBI:83333
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PAT42
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*fast stop DNA replication defect
*abolished DNA replication at high temperature
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|
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Biochemical Assay
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==Notes==
==References==
{{RefHelp}}
<references/>
[[Category:Publication]]
[[Category:To Be Converted]]

Latest revision as of 17:09, 22 June 2011

Citation

Nishimura, Y, Caro, L, Berg, CM and Hirota, Y (1971) Chromosome replication in Escherichia coli. IV. Control of chromosome replication and cell division by an integrated episome.J. Mol. Biol. 55:441-56

Abstract

No abstract in PubMed

Links

PubMed

Keywords

Integrative Suppression; Hfr; Phage; Acridine Orange; Ethidium Bromide; DNA Replication; Reversion; Temperature-sensitivity

Main Points of the Paper

  • New type of suppression of a temperature-sensitive mutation of DNA replication- the chromosome may be incorporated into the F replicon in Hfr strains

Materials and Methods Used

  • Acridine orange sensitivity assay
  • EtBr sensitivity assay
  • Cross-streak test for conjugation
  • Transduction

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.

Species Taxon ID Strain Gene (if known) OMP Phenotype Details Evidence Notes

Escherichia coli

NCBI:83333

CRT46

  • slow stop DNA replication defect
  • abolished initiation of DNA replication at high temperature

Biochemical Assay

Escherichia coli

NCBI:83333

CRT226

  • fast stop DNA replication defect
  • abolished DNA replication at high temperature

Biochemical Assay

Escherichia coli

NCBI:83333

PAT42

  • fast stop DNA replication defect
  • abolished DNA replication at high temperature

Biochemical Assay



Notes

References

See Help:References for how to manage references in OMPwiki.