Difference between revisions of "OMP ST:947 ! DH1 miniFparC10"

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*temperature:42C
 
*temperature:42C
*medium:Kanamycin (
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*medium:Kanamycin
 
*medium:L agar
 
*medium:L agar
 
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OMP_AN:726
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OMP:0007391
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abolished viability
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Relative to: OMP_AN:725
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<hr>Genotype differences:<br>
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no differences
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<hr>Condition differences:<br>
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medium:IPTG (100 uM)?
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*temperature:42C
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*medium:IPTG (100 uM)
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*medium:Kanamycin
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*medium:L agar
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ECO:0000181
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in vitro assay evidence
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PMID:9077455
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CHEBI:6104 Kanamycin
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CHEBI:61448 IPTG
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DNA nicking activity instead of transesterification
  
 
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Latest revision as of 15:16, 12 July 2016

Strain Summary

Strain Name

DH1 miniFparC10

Synonyms

parC10 mutant

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype
Strain Reference

PMID:9077455[1]

Strain availability
Ancestry

parent:DH1 miniFparC+

Annotated phenotypes




Notes

Phenotype Annotation

Annotation ID not OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:725

OMP:0007392

viable cell

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • temperature:42C
  • medium:Kanamycin
  • medium:L agar
ECO:0000181

in vitro assay evidence

PMID:9077455[1]

CHEBI:6104 Kanamycin

OMP_AN:726

OMP:0007391

abolished viability

Relative to: OMP_AN:725


Genotype differences:

no differences


Condition differences:

medium:IPTG (100 uM)?

  • temperature:42C
  • medium:IPTG (100 uM)
  • medium:Kanamycin
  • medium:L agar
ECO:0000181

in vitro assay evidence

PMID:9077455[1]

CHEBI:6104 Kanamycin CHEBI:61448 IPTG

DNA nicking activity instead of transesterification



Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. 1.0 1.1 1.2 Yokochi, T et al. (1996) DNA nicking by Escherichia coli topoisomerase IV with a substitution mutation from tyrosine to histidine at the active site. Genes Cells 1 1069-75 PubMed OMPwiki page