PMID:6819426

Citation	Manoil, C and Rosenbusch, JP (1982) Conjugation-deficient mutants of Escherichia coli distinguish classes of functions of the outer membrane OmpA protein.Mol. Gen. Genet. 187:148-56
Abstract	Sixty-two E. coli mutants, selected as being deficient as recipients in F factor conjugation, are altered either in the amount or function of the outer membrane OmpA protein or in lipopolysaccharide structure. These two components may function together in conjugation, since the residual conjugation activity of a mutant lacking OmpA protein was unaffected by the additional presence of a lipopolysaccharide defect. Sixty of the strains carried mutations mapping to ompA, and these could be divided into classes depending on the amount of OmpA protein in their membranes. Representatives of these classes of mutant alleles failed to complement in diploids, indicating that they all affect the ompA structural gene and nearby sequences needed for its expression. The properties of these classes distinguish three groups of OmpA protein functions: 1) the structural function in the outer membrane in providing resistance to chelating agents and the hydrophobic antibiotic novobiocin, 2) the receptor functions in phage TuII and K3 infection, and 3) the functions of binding cells together during conjugation, facilitating the uptake of receptor-bound colicin K or L, and allowing phage Ox2 to infect. Different cellular amounts or sites in OmpA protein are thus required for these three groups of functions.
Links	PubMed
Keywords	Bacterial Outer Membrane Proteins; Colicins; Coliphages; Conjugation, Genetic; Edetic Acid; Electrophoresis, Polyacrylamide Gel; Escherichia coli; F Factor; Genetic Complementation Test; Membrane Proteins; Mutation; Novobiocin
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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: A1 NCBI Taxon ID: 8333	Genotype of Reference Strain: ompA Genotype of Experimental Strain : ompA1	Reference Condition:	OMP:0000276	increased resistance to antibiotics	ECO:0000182	in vitro culture assay data	Resistant to novobicin
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: A1 NCBI Taxon ID: 8333	Genotype of Reference Strain: ompA Genotype of Experimental Strain : ompA1	Reference Condition:					Resistance to Chelator, specifically EDTA
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: CC205 NCBI Taxon ID: 8333	Genotype of Reference Strain:	Reference Condition:					Resistant to Phage K3hl, table 4
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: CC205 NCBI Taxon ID: 8333	Genotype of Reference Strain:	Reference Condition:					Resistant to Phage Ox2, table 4
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: CC205 NCBI Taxon ID: 8333	Genotype of Reference Strain:	Reference Condition:					Resistant to Phage K3, table 4
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: CC205 NCBI Taxon ID: 8333	Genotype of Reference Strain:	Reference Condition:					Resistant to Phage TUII*, table 4
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References

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