Difference between revisions of "PMID:400782"

From OMPwiki
(Notes)
(Notes)
 
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OMP term:
 
OMP term:
 
Mesophilic growth - term is being developed.
 
Mesophilic growth - term is being developed.
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Rifampin- term being developed.
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 +
Eco:
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both in vitro assays
  
 
==References==
 
==References==

Latest revision as of 18:44, 14 June 2013

Citation

Bagdasarian, MM, Izakowska, M and Bagdasarian, M (1977) Suppression of the DnaA phenotype by mutations in the rpoB cistron of ribonucleic acid polymerase in Salmonella typhimurium and Escherichia coli. J. Bacteriol. 130:577-82

Abstract

A class of mutations that confer resistance to rifampin in Salmonella typhimurium and Escherichia coli also suppresses the thermosensitivity of chromosome initiation in dnaA mutants. Ribonucleic acid polymerase is resistant to rifampin in vitro in these suppressive mutants, and the suppressors of dnaA cannot be separated from the rpoB mutations by transduction. It is concluded, therefore, that certain rpoB mutations may suppress the DnaA phenotype.

Links

PubMed PMC235255

Keywords


Main Points of the Paper

Please summarize the main points of the paper.

  • Mutations in rpoB suppress a dnaA phenotype.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: EC3
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: dnaA
  • Genotype of Experimental Strain : dnaA46 in strain EC8
  • Reference Condition:

Increased resistance to rifampin. Table 2.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: EC3
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: dnaA rpoB
  • Genotype of Experimental Strain : dnaA46 rpoB706 in strain EC8
  • Reference Condition: non permissive temp (42.5C) of the single mutant dnaA

DnaA1 Mutant is unable to grow at 42.5C, mutation in rpoB suppresses thermosensitivity allowing the strain to grow at 42.5C.


Notes

OMP term: Mesophilic growth - term is being developed. Rifampin- term being developed.

Eco: both in vitro assays

References

See Help:References for how to manage references in OMPwiki.