Difference between revisions of "PMID:330501"

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'''Hobson, AC, Gho, D and Müller-Hill, B'''  (1977) Isolation, genetic analysis, and characterization of Escherichia coli mutants with defects in the lacY gene. ''J. Bacteriol.'' '''131''':830-8
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Five hundred thirty-five lacY mutants were isolated from an Escherichia coli strain carrying the lactose operon on an F' factor, either without mutagenesis or after mutagenesis with 2-aminopurine or N-methyl-N'-nitro-N-nitrosoguanidine. Crosses against 48 independently isolated deletions ending in the lacY gene divided the gene into 36 deletion groups. Suppressibility studies with 7 nonsense suppressor strains classified 276 mutants as nonsense mutants and 78 as missense (or nonsuppressible) mutants. One hundred seventy-nine mutants were "leaky" and could not be so allocated, and two were found to have small internal deletions. Nonsense mutants could in many cases be subdivided even within deletion groups on the basis of their suppressibility pattern, giving a total of 70 groups of nonsense mutants. Studies of these mutants allow the following conclusions: lactose and melibiose most probably do not have separate binding sites on the permease; the lacY region most likely consists of one cistron, and so both active transport and facilitated diffusion are functions of one protein; and finally, there is probably no small defined region of the permease responsible for energy coupling of transport. Furthermore, the strains and the analysis form the basis for a future functional study of the permease by biochemical techniques.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=330501 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC235538 PMC235538]
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2-Aminopurine; Biological Transport, Active; Chromosome Mapping; Diffusion; Disaccharides/metabolism; Escherichia coli/enzymology; Escherichia coli/isolation & purification; Escherichia coli/metabolism; Galactosidases/metabolism; Genes; Lactose/metabolism; Membrane Transport Proteins/metabolism; Methylnitronitrosoguanidine; Mutagens; Mutation; Suppression, Genetic
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="W51ce134674fa7"  class=" tableEdit Phenotype_Table_2" 
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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==Notes==
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==References==
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[[Category:Publication]]

Revision as of 17:50, 28 June 2013

Citation

Hobson, AC, Gho, D and Müller-Hill, B (1977) Isolation, genetic analysis, and characterization of Escherichia coli mutants with defects in the lacY gene. J. Bacteriol. 131:830-8

Abstract

Five hundred thirty-five lacY mutants were isolated from an Escherichia coli strain carrying the lactose operon on an F' factor, either without mutagenesis or after mutagenesis with 2-aminopurine or N-methyl-N'-nitro-N-nitrosoguanidine. Crosses against 48 independently isolated deletions ending in the lacY gene divided the gene into 36 deletion groups. Suppressibility studies with 7 nonsense suppressor strains classified 276 mutants as nonsense mutants and 78 as missense (or nonsuppressible) mutants. One hundred seventy-nine mutants were "leaky" and could not be so allocated, and two were found to have small internal deletions. Nonsense mutants could in many cases be subdivided even within deletion groups on the basis of their suppressibility pattern, giving a total of 70 groups of nonsense mutants. Studies of these mutants allow the following conclusions: lactose and melibiose most probably do not have separate binding sites on the permease; the lacY region most likely consists of one cistron, and so both active transport and facilitated diffusion are functions of one protein; and finally, there is probably no small defined region of the permease responsible for energy coupling of transport. Furthermore, the strains and the analysis form the basis for a future functional study of the permease by biochemical techniques.

Links

PubMed PMC235538

Keywords

2-Aminopurine; Biological Transport, Active; Chromosome Mapping; Diffusion; Disaccharides/metabolism; Escherichia coli/enzymology; Escherichia coli/isolation & purification; Escherichia coli/metabolism; Galactosidases/metabolism; Genes; Lactose/metabolism; Membrane Transport Proteins/metabolism; Methylnitronitrosoguanidine; Mutagens; Mutation; Suppression, Genetic

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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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Notes

References

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