PMID:1846149
Citation |
Karow, M, Fayet, O, Cegielska, A, Ziegelhoffer, T and Georgopoulos, C (1991) Isolation and characterization of the Escherichia coli htrB gene, whose product is essential for bacterial viability above 33 degrees C in rich media. J. Bacteriol. 173:741-50 |
---|---|
Abstract |
We have identified and studied the htrB gene of Escherichia coli. Insertional inactivation of the htrB gene leads to bacterial death at temperatures above 33 degrees C. The mutant bacterial phenotype at nonpermissive temperatures includes an arrest of cell division followed by the formation of bulges or filaments. The htrB+ gene has been cloned by complementation and shown to reside at 23.4 min on the E. coli genetic map, the relative order of the neighboring loci being mboA-htrB-pyrC. The htrB gene is transcribed in a counterclockwise fashion, relative to the E. coli genetic map, and its product has been identified as a membrane-associated protein of 35,000 Da. Growth experiments in minimal media indicate that the HtrB function becomes dispensable at low growth rates. |
Links | |
Keywords |
Cloning, Molecular; Coliphages/genetics; Culture Media; DNA Transposable Elements; Escherichia coli/cytology; Escherichia coli/genetics; Escherichia coli/growth & development; Genes, Bacterial; Genomic Library; Genotype; Kinetics; Plasmids; Restriction Mapping; Temperature |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
---|---|---|---|---|---|---|---|---|---|
Notes
References
See Help:References for how to manage references in OMPwiki.