Difference between revisions of "PMID:1495389"
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| + | !align=left align='left' bgcolor='#CCCCFF' |Citation | ||
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| + | '''Allaoui, A, Mounier, J, Prévost, MC, Sansonetti, PJ and Parsot, C''' (1992) icsB: a Shigella flexneri virulence gene necessary for the lysis of protrusions during intercellular spread. ''Mol. Microbiol.'' '''6''':1605-16 | ||
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| + | !align=left align='left' bgcolor='#CCCCFF' |Abstract | ||
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| + | Shigella flexneri causes bacillary dysentery by invading epithelial cells of the colonic mucosa. We have characterized the icsB gene which is located on the virulence plasmid pWR100. After inactivation of icsB, the mutant strain remained invasive, but formed abnormally small plaques on HeLa cell monolayers, colonized only the peripheral cells of Caco-2 islets, and was unable to provoke a keratoconjunctivitis in guinea-pigs. Examination of infected HeLa cells showed that the icsB mutant was able to lyse the phagocytic vacuole and to form protrusions at the surface of infected cells, but, unlike the wild type, remained trapped in protrusions surrounded by two membranes. These results indicate that IcsB is involved in the lysis of the protrusions, a step necessary for intercellular spread. | ||
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| + | [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1495389 PubMed] | ||
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| + | !align=left align='left' bgcolor='#CCCCFF' |Keywords | ||
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| + | Amino Acid Sequence; Bacterial Proteins/isolation & purification; Bacteriolysis/genetics; Base Sequence; Extracellular Space/microbiology; Gene Expression Regulation, Bacterial/physiology; Genes, Bacterial/genetics; Genes, Bacterial/physiology; HeLa Cells/microbiology; Humans; Microscopy, Electron; Molecular Sequence Data; Mutation; Open Reading Frames/physiology; Recombinant Fusion Proteins/isolation & purification; Shigella flexneri/genetics; Shigella flexneri/pathogenicity; Shigella flexneri/ultrastructure; Temperature; Virulence/genetics; Virulence/physiology | ||
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| + | ==Main Points of the Paper == | ||
| + | {{LitSignificance}} | ||
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| + | == Materials and Methods Used == | ||
| + | {{LitMaterials}} | ||
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| + | ==Phenotype Annotations== | ||
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| + | !|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | ||
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| + | ==Notes== | ||
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| + | ==References== | ||
| + | {{RefHelp}} | ||
| + | <references/> | ||
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| + | [[Category:Publication]] | ||
Latest revision as of 19:59, 17 February 2019
| Citation |
Allaoui, A, Mounier, J, Prévost, MC, Sansonetti, PJ and Parsot, C (1992) icsB: a Shigella flexneri virulence gene necessary for the lysis of protrusions during intercellular spread. Mol. Microbiol. 6:1605-16 |
|---|---|
| Abstract |
Shigella flexneri causes bacillary dysentery by invading epithelial cells of the colonic mucosa. We have characterized the icsB gene which is located on the virulence plasmid pWR100. After inactivation of icsB, the mutant strain remained invasive, but formed abnormally small plaques on HeLa cell monolayers, colonized only the peripheral cells of Caco-2 islets, and was unable to provoke a keratoconjunctivitis in guinea-pigs. Examination of infected HeLa cells showed that the icsB mutant was able to lyse the phagocytic vacuole and to form protrusions at the surface of infected cells, but, unlike the wild type, remained trapped in protrusions surrounded by two membranes. These results indicate that IcsB is involved in the lysis of the protrusions, a step necessary for intercellular spread. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacterial Proteins/isolation & purification; Bacteriolysis/genetics; Base Sequence; Extracellular Space/microbiology; Gene Expression Regulation, Bacterial/physiology; Genes, Bacterial/genetics; Genes, Bacterial/physiology; HeLa Cells/microbiology; Humans; Microscopy, Electron; Molecular Sequence Data; Mutation; Open Reading Frames/physiology; Recombinant Fusion Proteins/isolation & purification; Shigella flexneri/genetics; Shigella flexneri/pathogenicity; Shigella flexneri/ultrastructure; Temperature; Virulence/genetics; Virulence/physiology |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
Notes
References
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