PMID:137233

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Citation

Deutch, CE, Scarpulla, RC, Sonnenblick, EB and Soffer, RL (1977) Pleiotropic phenotype of an Escherichia coli mutant lacking leucyl-, phenylalanyl-transfer ribonucleic acid-protein transferase. J. Bacteriol. 129:544-6

Abstract

A mutant of Escherichia coli that lacks leucyl-, phenylalanyl-transfer ribonucleic acid-protein transferase had diminished activities of L-phenylalanyl-transfer ribonucleic acid synthetase and tryptophanase, grew faster than its parent with aspartic acid as the sole nitrogen source, accumulated higher levels of enterochelin in the medium during iron limitation, and exhibited an abnormal morphology.

Links

PubMed PMC234957

Keywords

Acyltransferases/metabolism; Aspartic Acid/metabolism; Enterobactin/metabolism; Escherichia coli/cytology; Escherichia coli/enzymology; Escherichia coli/metabolism; Mutation; Phenylalanine-tRNA Ligase/metabolism; Tryptophanase/metabolism

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: W4977
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: aat
  • Genotype of Experimental Strain : aat-1
  • Reference Condition:
OMP:0000132

pleomorphic

ECO:0000176

mutant visible phenotype evidence

Abnormal Morphology appearing as clubs, spheres or ellipsoids.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: W4977
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: aat
  • Genotype of Experimental Strain : aat-1
  • Reference Condition: Aspartic Acid as Sole Nitrogen Source
  • Experimental Condition: Aspartic Acid as sole Nitrogen Source
OMP:0000282

increased growth rate

ECO:0000005

enzyme assay evidence

In the presence of Aspartic Acid as the sole nitrogen source, the mutants doubling time increase by twice.


Notes

  • OMP Cell shape: What exists implies a state, thus there is no relative phenotypes. Must create phenotypes for all shapes keeping in mind that some colonies will have a mixed and oddly shaped mutants that might not fall into predefined shapes we create.
  • Marcus was going to think about this since this node might need a complete overhaul to take into account disjoint relationships.

References

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