OMP ST:985 ! Escherichia coli K-12 JW2315

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 JW2315

Synonyms

JW2315-2

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 truA727(del)::kan

Strain Reference

Coli Genetic Stock Center

Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:898

OMP:0007092

decreased cell motility by swarming

Relative to: OMP_AN:881


Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +truA727(del) -IN(rrnD-rrnE)1


Condition differences:

no differences

  • temperature:33C
  • medium:swarming motility medium
ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:31481

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27601


Genotype differences:

+lacZ4787(del)( +truA727(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Sodium Acetate anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32954) Sodium Acetate anhydrous

  • chemical concentration= 0.6%
  • normalized fitness= -3.77

OMP_AN:31482

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27611


Genotype differences:

+lacZ4787(del)( +truA727(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Sodium Acetate anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32954) Sodium Acetate anhydrous

  • chemical concentration= 0.6%
  • normalized fitness= -3.77

OMP_AN:31483

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27603


Genotype differences:

+lacZ4787(del)( +truA727(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -6.54

OMP_AN:31484

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27613


Genotype differences:

+lacZ4787(del)( +truA727(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -6.54

OMP_AN:31485

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27510


Genotype differences:

+lacZ4787(del)( +truA727(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Mitomycin C
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:27504) Mitomycin C

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= -3.33

OMP_AN:31486

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27570


Genotype differences:

+lacZ4787(del)( +truA727(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Propidium iodide
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:51240) Propidium iodide

  • chemical concentration= 50 ug/ml
  • normalized fitness= 3.85

OMP_AN:31487

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27546


Genotype differences:

+lacZ4787(del)( +truA727(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Pyocyanin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8653) Pyocyanin

  • chemical concentration= 10 ug/ml
  • normalized fitness= -7.63

OMP_AN:31488

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27533


Genotype differences:

+lacZ4787(del)( +truA727(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Carbenicillin disodium
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:34609) Carbenicillin disodium

  • chemical concentration= 1.5 ug/ml
  • normalized fitness= -3.54

OMP_AN:31489

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27544


Genotype differences:

+lacZ4787(del)( +truA727(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Chloramphenicol
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17698) Chloramphenicol

  • chemical concentration= 2 ug/ml
  • normalized fitness= -4.99

OMP_AN:31490

OMP:0007008

decreased population growth at acidic pH

Relative to: OMP_AN:27622


Genotype differences:

+lacZ4787(del)( +truA727(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:MES-HOMOPIPES
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (**SID:329817955 SID:57651422) MES-HOMOPIPES

  • chemical concentration= pH 4
  • normalized fitness= -4.83


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Inoue, T et al. (2007) Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. J. Bacteriol. 189 950-7 PubMed OMPwiki page
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 2.9 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page