OMP ST:983 ! Escherichia coli K-12 JW5531

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 JW5531

Synonyms

JW5531-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 deaD774(del)::kan

Strain Reference

Coli Genetic Stock Center

Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:896

OMP:0007092

decreased cell motility by swarming

Relative to: OMP_AN:881


Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +deaD774(del) -IN(rrnD-rrnE)1


Condition differences:

no differences

  • temperature:33C
  • medium:swarming motility medium
ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:36236

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27530


Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Amoxicillin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:2676) Amoxicillin

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= -3.38

OMP_AN:36237

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27552


Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azidothymidine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:10110) Azidothymidine

  • chemical concentration= 0.5 ng/ml
  • normalized fitness= -3.55

OMP_AN:36238

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27535


Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefsulodin sodium salt hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31380) Cefsulodin sodium salt hydrate

  • chemical concentration= 18 ug/ml
  • normalized fitness= -5.12

OMP_AN:36239

OMP:0006037

increased resistance to macrolide

Relative to: OMP_AN:27520


Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Erythromycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42355) Erythromycin

  • chemical concentration= 5 ug/ml
  • normalized fitness= 3.95

OMP_AN:36240

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27545


Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Isoniazid
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:6030) Isoniazid

  • chemical concentration= 1 mM
  • normalized fitness= -3.74

OMP_AN:36241

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27510


Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Mitomycin C
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:27504) Mitomycin C

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= -4.65

OMP_AN:36242

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27600


Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= 3.34

OMP_AN:36243

OMP:0007033

increased utilization of inorganic nitrogen source

Relative to: OMP_AN:27610


Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= 3.34

OMP_AN:36244

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27580


Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nickel stress-Ni(II)Cl2 hexahydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:53542) Nickel stress-Ni(II)Cl2 hexahydrate

  • chemical concentration= 0.1 mM (LB)
  • normalized fitness= -3.73

OMP_AN:36245

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27503


Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nitrofurantoin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:71415) Nitrofurantoin

  • chemical concentration= 1.5 ug/ml
  • normalized fitness= -5.93

OMP_AN:36246

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27574


Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Paraquat dichloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28786) Paraquat dichloride

  • chemical concentration= 5 uM
  • normalized fitness= -3.89

OMP_AN:36247

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27564


Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Phleomycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75044) Phleomycin

  • chemical concentration= 1 ug/ml
  • normalized fitness= -3.88

OMP_AN:36248

OMP:0007896

decreased resistance to a surfactant

Relative to: OMP_AN:27582


Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:SDS
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8984) SDS

  • chemical concentration= 1 %
  • normalized fitness= -4.62

OMP_AN:36249

OMP:0006040

decreased resistance to quinolone

Relative to: OMP_AN:27543


Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Streptonigrin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9287) Streptonigrin

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= -3.89

OMP_AN:36250

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27588


Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Triclosan/Irgasan
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:164200) Triclosan/Irgasan

  • chemical concentration= 0.05 ug/ml
  • normalized fitness= 3.23

OMP_AN:36251

OMP:0006041

increased resistance to quinolone

Relative to: OMP_AN:27539


Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Ciprofloxacin hydrochloride hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:59936) Ciprofloxacin hydrochloride hydrate

  • chemical concentration= 0.008 ug/ml
  • normalized fitness= 3.85

OMP_AN:36252

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27569


Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EtOH
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:16236) EtOH

  • chemical concentration= 6 %
  • normalized fitness= -3.18

OMP_AN:36253

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27598


Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= 2.98

OMP_AN:36254

OMP:0007888

increased resistance to a metal

Relative to: OMP_AN:27608


Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= 2.98

OMP_AN:36255

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27599


Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= 3.32

OMP_AN:36256

OMP:0007936

increased resistance to nutrient limitation

Relative to: OMP_AN:27609


Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= 3.32

OMP_AN:36257

OMP:0006041

increased resistance to quinolone

Relative to: OMP_AN:27541


Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nalidixic acid sodium salt
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:3864541) Nalidixic acid sodium salt

  • chemical concentration= 2 ug/ml
  • normalized fitness= 3.3

OMP_AN:36258

OMP:0006057

decreased resistance to aminoglycoside

Relative to: OMP_AN:27527


Genotype differences:

+lacZ4787(del)( +deaD774(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Spectinomycin dihydrochloride pentahydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9217) Spectinomycin dihydrochloride pentahydrate

  • chemical concentration= 4 ug/ml
  • normalized fitness= -3.44


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Inoue, T et al. (2007) Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. J. Bacteriol. 189 950-7 PubMed OMPwiki page
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 2.22 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page