OMP ST:926 ! Escherichia coli K-12 JW5248

From OMPwiki

Strain Summary

Strain Name

Escherichia_coli_K-12_JW5248

Synonyms

BW25113 marR751(del)::kan, ECK1523

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 marR751(del)::kan

Strain Reference
Strain availability

CGSC

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes


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Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:666

OMP:0006045

increased resistance to beta-lactam compound

Relative to: OMP_AN:665

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:mecillinam (0.12 ug ml-1)

PMID:21185072[2]

CHEBI:51208 mecillinam

fitness score = 6.61

OMP_AN:667

OMP:0006068

increased resistance to chloramphenicol

Relative to: OMP_AN:649

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:chloramphenicol (2.0 ug ml-1)

PMID:21185072[2]

CHEBI:17698 chloramphenicol

fitness score = 5.13

OMP_AN:669

OMP:0006063

increased resistance to tetracycline

Relative to: OMP_AN:668

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:minocycline (1.0 ug ml-1)

PMID:21185072[2]

CHEBI:50694 minocycline

fitness score = 4.89

OMP_AN:671

OMP:0000334

increased resistance to aminopyrimidine

Relative to: OMP_AN:670

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:trimethoprim (0.2 ug ml-1)

PMID:21185072[2]

CHEBI:45924 trimethoprim

fitness score = 4.46

OMP_AN:672

OMP:0006063

increased resistance to tetracycline

Relative to: OMP_AN:647

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:tetracycline (1 ug ml-1)

PMID:21185072[2]

CHEBI:27902 tetracycline

fitness score = 4.22

OMP_AN:673

OMP:0006041

increased resistance to quinolone

Relative to: OMP_AN:653

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:streptonigrin (0.5 ug ml-1)

PMID:21185072[2]

CHEBI:9287 streptonigrin

fitness score = 4.09

OMP_AN:675

OMP:0006063

increased resistance to tetracycline

Relative to: OMP_AN:870

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:doxycycline (1.0 ug ml-1)

PMID:21185072[2]

CHEBI:50845 doxycycline

fitness score = 4.03

OMP_AN:676

OMP:0007384

increased resistance to a benzamide

Relative to: OMP_AN:659

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:CHIR-090 (0.075 ug ml-1)

PMID:21185072[2]

PubChem CID:11546620 Chir-090

fitness score = 3.93

OMP_AN:678

OMP:0006041

increased resistance to quinolone

Relative to: OMP_AN:677

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:ciprofloxacin (0.004 ug ml-1)

PMID:21185072[2]

CHEBI:100241 ciprofloxacin

fitness score = 3.70

OMP_AN:680

OMP:0007171

resistant to antimicrobial peptide

Relative to: OMP_AN:679

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:bleomycin (0.5 ug ml-1)

PMID:21185072[2]

CHEBI:22907 bleomycin

fitness score = 3.50

OMP_AN:682

OMP:0006037

increased resistance to macrolide

Relative to: OMP_AN:681

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:azithromycin (1.0 ug ml-1)

PMID:21185072[2]

CHEBI:2955 azithromycin

fitness score = 3.30

OMP_AN:684

OMP:0006037

increased resistance to macrolide

Relative to: OMP_AN:683

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:erythromycin (5.0 ug ml-1)

PMID:21185072[2]

CHEBI:48923 erythromycin

fitness score = 3.29

OMP_AN:686

OMP:0007376

increased resistance to alkylating agent

Relative to: OMP_AN:685

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:MMS (0.05%)

PMID:21185072[2]

CHEBI:25255 MMS

fitness score = 8.56

OMP_AN:688

OMP:0007362

Increased resistance to a nucleoside

Relative to: OMP_AN:687

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:azidothymidine (1.0 ug ml-1)

PMID:21185072[2]

CHEBI:10110 azidothymidine

fitness score = 3.73

OMP_AN:690

OMP:0007249

Increased sensitivity to a chemical

Relative to: OMP_AN:689

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:propidium iodide (50 ug ml-1)

PMID:21185072[2]

CHEBI:51240 propidium iodide

fitness score = 5.15

OMP_AN:698

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:697

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:cefoxitin (0.5 ug ml-1)

PMID:21185072[2]

CHEBI:209807 cefoxitin

fitness score = -3.14

OMP_AN:700

OMP:0007219

decreased resistance to rifamycin

Relative to: OMP_AN:699

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:rifampicin (2.0 ug ml-1)

PMID:21185072[2]

CHEBI:28077 rifampicin

fitness score = -3.29

OMP_AN:816

OMP:0005276

decreased resistance to organic heterocyclic compound

Relative to: OMP_AN:815

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:A22 (15 ug ml-1)

PMID:21185072[2]

CID:16741200 A22

fitness score = -3.78

OMP_AN:819

OMO:0005345

Relative to: OMP_AN:817

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:procaine (30 ug ml-1)

PMID:21185072[2]

CHEBI:8430 procaine

fitness score = -3.39

OMP_AN:831

OMP:0007250

decreased sensitivity to a chemical

Relative to: OMP_AN:827

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:ethanol (2.0 ug ml-1)

PMID:21185072[2]

CHEBI:16236 ethanol

fitness score = -3.78

OMP_AN:832

OMP:0007250

decreased sensitivity to a chemical

Relative to: OMP_AN:828

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:bile 0.5%

PMID:21185072[2]

CHEBI:22868 bile

fitness score = -3.51

OMP_AN:833

OMP:0007250

decreased sensitivity to a chemical

Relative to: OMP_AN:829

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:glycerol

PMID:21185072[2]

CHEBI:17754 glycerol

fitness score = -3.45

OMP_AN:834

OMP:0007250

decreased sensitivity to a chemical

Relative to: OMP_AN:830

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:maltose

PMID:21185072[2]

CHEBI:17306 maltose

fitness score = -3.05

OMP_AN:7514

OMP:0005347

Relative to: OMP_AN:7500

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:triclosan (0.05 ug ml-1)

PMID:21185072[2]

CHEBI:164200 triclosan

fitness score = 4.25

OMP_AN:7562

OMP:0005347

increased resistance to organic aromatic compounds

Relative to: OMP_AN:7495

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

medium:benzalkonium (25 ug ml-!)

PMID:21185072[2]

CHEBI:3020 benzalkonium

fitness score = 3.09

OMP_AN:35883

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27552

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azidothymidine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:10110) Azidothymidine

  • chemical concentration= 1 ng/ml
  • normalized fitness= 3.73

OMP_AN:35884

OMP:0006037

increased resistance to macrolide

Relative to: OMP_AN:27518

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azithromycin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:2955) Azithromycin

  • chemical concentration= 1 ug/ml
  • normalized fitness= 3.3

OMP_AN:35885

OMP:0007895

increased resistance to a surfactant

Relative to: OMP_AN:27581

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Benzalkonium chloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3020) Benzalkonium chloride

  • chemical concentration= 25 ug/ml
  • normalized fitness= 3.09

OMP_AN:35886

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27587

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:26711 + CHEBI:9177) Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)

  • chemical concentration= 0.5 %
  • normalized fitness= -3.51

OMP_AN:35887

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27561

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bleomycin sulfate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:34582) Bleomycin sulfate

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= 3.5

OMP_AN:35888

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27604

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= -3.45

OMP_AN:35889

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27614

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= -3.45

OMP_AN:35890

OMP:0007376

increased resistance to an alkylating agent

Relative to: OMP_AN:27502

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:methyl methanesulfonate (MMS)
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:25255) methyl methanesulfonate (MMS)

  • chemical concentration= 0.05%
  • normalized fitness= 8.56

OMP_AN:35891

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27605

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= -3.05

OMP_AN:35892

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27615

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= -3.05

OMP_AN:35893

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27570

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Propidium iodide
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:51240) Propidium iodide

  • chemical concentration= 50 ug/ml
  • normalized fitness= 5.15

OMP_AN:35894

OMP:0006041

increased resistance to quinolone

Relative to: OMP_AN:27543

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Streptonigrin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9287) Streptonigrin

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= 4.09

OMP_AN:35895

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27588

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Triclosan/Irgasan
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:164200) Triclosan/Irgasan

  • chemical concentration= 0.05 ug/ml
  • normalized fitness= 4.25

OMP_AN:35896

OMP:0006041

increased resistance to quinolone

Relative to: OMP_AN:27539

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Ciprofloxacin hydrochloride hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:59936) Ciprofloxacin hydrochloride hydrate

  • chemical concentration= 0.004 ug/ml
  • normalized fitness= 3.7

OMP_AN:35897

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27569

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EtOH
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:16236) EtOH

  • chemical concentration= 2 %
  • normalized fitness= -3.78

OMP_AN:35898

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27500

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Trimethoprim
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:45924) Trimethoprim

  • chemical concentration= 0.2 ug/ml
  • normalized fitness= 4.46

OMP_AN:35899

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27591

Genotype differences:

+lacZ4787(del)( +marR751(del) -lacZ4787(del)

Condition differences:

no differences

ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:45924 + CHEBI:9331) Trimethoprim (CHEBI:45924) + Sulfamethizole (CHEBI:9331)

  • chemical concentration= 0.1 + 50 ug/ml
  • normalized fitness= -4.1
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 2.22 2.23 2.24 2.25 2.26 2.27 2.28 2.29 2.30 2.31 2.32 2.33 2.34 2.35 2.36 2.37 2.38 2.39 2.40 2.41 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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