OMP ST:922 ! Escherichia coli K-12 MS1350
Contents
Strain Summary
| Strain Name |
MS1350 |
|---|---|
| Synonyms | |
| Taxon Information |
|
| Genotype |
F- lambda- e14-(?) Rac-0(?) uvrC34 hisG4(Oc) rfbC1 mgl-51 rpsL31(strR) kdgK51 xylA5 mtl-1 argE3(Oc) thiE1 thyA galU51 |
| Strain Reference | |
| Strain availability | |
| Ancestry | |
| Annotated phenotypes |
|
Notes
"Strain MS1350 was prepared by M. Silverman in M. Simon's laboratory from a K-12 strain, AB1884, obtained from J. Adler. The strain was made Lac+, Pro+, Gal+, Thr+,and Leu+ by conjugation with strain KL96, and then Trp- by bromodeoxyuridine auxotroph selection (4). The galU marker was introduced by P1 transduction from W4597 by selecting for Trp+ Gal- recombinants. Thy- strains were obtained by trimethoprim treatment (19). The absence of suppressors was determined by the inability of phage T4 amber B22 to form plaques on this strain." [2]
AB1884 is Rac-0, and KL96 e14-. I haven't been able to determine from the literature whether MS1350 was checked for either Rac-0 or e14-, so in the genotype these alleles are marked with question marks. Siegele (talk) 15:45, 20 July 2016 (CDT)
Phenotype Annotation
| Annotation ID | not | OMP ID | OMP term name | Relative phenotype information | Experimental condition | ECO ID | ECO term name | Reference | Annotation Extension | Notes |
|---|---|---|---|---|---|---|---|---|---|---|
|
OMP_AN:718 |
OMP:0000005 |
presence of cell motility |
Relative to: Genotype differences: Nothing to compare Condition differences: Nothing to compare |
medium:Motility agar |
swim plate assay |
|||||
|
OMP_AN:719 |
Presence of flagella |
Relative to: Genotype differences: Nothing to compare Condition differences: Nothing to compare |
ECO:0001098 |
microscopy evidence |
||||||
|
OMP_AN:737 |
OMP:0007238 |
sensitive to bacteriophage |
Relative to: Genotype differences: Nothing to compare Condition differences: Nothing to compare |
| ||||||
Additional Information
Links
References
See Help:References for how to manage references in OMPwiki.
- ↑ Komeda, Y et al. (1977) Genetic analysis of Escherichia coli K-12 region I flagellar mutants. J. Bacteriol. 131 801-8 PubMed OMPwiki page
- ↑ 2.0 2.1 2.2 2.3 2.4 Silverman, M & Simon, M (1973) Genetic analysis of flagellar mutants in Escherichia coli. J. Bacteriol. 113 105-13 PubMed OMPwiki page
