OMP ST:8452 ! Escherichia coli K-12 JW3584

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Strain Summary

Strain Name

Escherichia coli K-12 JW3584

Synonyms
Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 secB721(del)::FRT-kan-FRT

Strain Reference

PMID:19160513[1]

Strain availability
Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes


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Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:33725

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27530

Genotype differences:

+lacZ4787(del)( +secB721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Amoxicillin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:2676) Amoxicillin

  • chemical concentration= 1.5 ug/ml
  • normalized fitness= -3.85

OMP_AN:33726

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27549

Genotype differences:

+lacZ4787(del)( +secB721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:CHIR-090
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:134107 CID:11546620) CHIR-090

  • chemical concentration= 0.02 ug/ml
  • normalized fitness= -4.53

OMP_AN:33727

OMP:0006045

increased resistance to beta-lactam compound

Relative to: OMP_AN:27534

Genotype differences:

+lacZ4787(del)( +secB721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefoxitin sodium hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3501) Cefoxitin sodium hydrate

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= 3.82

OMP_AN:33728

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27585

Genotype differences:

+lacZ4787(del)( +secB721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cholate sodium hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23679061) Cholate sodium hydrate

  • chemical concentration= 2 %
  • normalized fitness= -6.6

OMP_AN:33729

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27579

Genotype differences:

+lacZ4787(del)( +secB721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Copper stress-CuCl2 dihydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:86318) Copper stress-CuCl2 dihydrate

  • chemical concentration= 4 mM (LB)
  • normalized fitness= -7.63

OMP_AN:33730

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27501

Genotype differences:

+lacZ4787(del)( +secB721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Dibucaine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:247956) Dibucaine

  • chemical concentration= 1.2 mM
  • normalized fitness= -5.76

OMP_AN:33731

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27570

Genotype differences:

+lacZ4787(del)( +secB721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Propidium iodide
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:51240) Propidium iodide

  • chemical concentration= 50 ug/ml
  • normalized fitness= 3.94

OMP_AN:33732

OMP:0006041

increased resistance to quinolone

Relative to: OMP_AN:27543

Genotype differences:

+lacZ4787(del)( +secB721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Streptonigrin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9287) Streptonigrin

  • chemical concentration= 0.5 ug/ml
  • normalized fitness= 3.52

OMP_AN:33733

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27569

Genotype differences:

+lacZ4787(del)( +secB721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EtOH
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:16236) EtOH

  • chemical concentration= 6 %
  • normalized fitness= -7.42

OMP_AN:33734

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27504

Genotype differences:

+lacZ4787(del)( +secB721(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Novobiocin sodium salt
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31924) Novobiocin sodium salt

  • chemical concentration= 8 ug/ml
  • normalized fitness= -5.78
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Typas, A et al. (2008) High-throughput, quantitative analyses of genetic interactions in E. coli. Nat. Methods 5 781-7 PubMed OMPwiki page
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 2.9 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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