Difference between revisions of "OMP ST:74667 ! Virtual reference strain for Nichols RJ (2011) Cell 144, 143-156."
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==Notes== | ==Notes== | ||
+ | OMP_ST:74667 was created to use as the reference (or control) strain for the high-throughput phenotype studies of Nichols et al. (2011. Cell. 144:143-156). | ||
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+ | Of the 4,602 strains that were screened, 4,502 strains were derivatives of E. coli K-12 BW25113; the remaining 100 strains were derivative of E. coli K-12 MG1655. For this reason, we have used the genotype of BW25113 as the genotype for the collection. | ||
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+ | The collection of mutants that was screened included 4334 deletions of non-essential genes (the Keio Collection ([1]), 148 SPA-tagged derivatives of essential genes ([2] [3]), 9 point-mutant alleles of essential genes (as well as the corresponding control strains that carried the same antibioticR marker linked to the wild-type allele), 2 truncations of essential genes, and 100 deletions of small RNAs and small proteins (,ref name='PMID:19734312'/>). See the original paper for additional details about the strains used in the screen. | ||
==Phenotype Annotation== | ==Phenotype Annotation== |
Revision as of 18:43, 8 November 2018
Contents
Strain Summary
Strain Name |
Virtual reference strain for Nichols RJ (2011) Cell 144, 143-156. |
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Synonyms | |
Taxon Information |
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Genotype | |
Strain Reference | |
Strain availability | |
Ancestry | |
Annotated phenotypes |
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Notes
OMP_ST:74667 was created to use as the reference (or control) strain for the high-throughput phenotype studies of Nichols et al. (2011. Cell. 144:143-156).
Of the 4,602 strains that were screened, 4,502 strains were derivatives of E. coli K-12 BW25113; the remaining 100 strains were derivative of E. coli K-12 MG1655. For this reason, we have used the genotype of BW25113 as the genotype for the collection.
The collection of mutants that was screened included 4334 deletions of non-essential genes (the Keio Collection ([1]), 148 SPA-tagged derivatives of essential genes ([2] [3]), 9 point-mutant alleles of essential genes (as well as the corresponding control strains that carried the same antibioticR marker linked to the wild-type allele), 2 truncations of essential genes, and 100 deletions of small RNAs and small proteins (,ref name='PMID:19734312'/>). See the original paper for additional details about the strains used in the screen.
Phenotype Annotation
Annotation ID | Qualifier | OMP ID | OMP term name | Relative phenotype information | Experimental condition | ECO ID | ECO term name | Reference | Annotation Extension | Notes |
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Additional Information
Links
References
See Help:References for how to manage references in OMPwiki.
- ↑ Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page