OMP ST:73870 ! Escherichia coli K-12 JW4129

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 JW4129

Synonyms

JW4129-2

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 miaA721(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:35148

OMP:0006045

increased resistance to beta-lactam compound

Relative to: OMP_AN:27534


Genotype differences:

+lacZ4787(del)( +miaA721(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefoxitin sodium hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3501) Cefoxitin sodium hydrate

  • chemical concentration= 0.75 ug/ml
  • normalized fitness= 3.36

OMP_AN:35149

OMP:0006045

increased resistance to beta-lactam compound

Relative to: OMP_AN:27535


Genotype differences:

+lacZ4787(del)( +miaA721(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefsulodin sodium salt hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31380) Cefsulodin sodium salt hydrate

  • chemical concentration= 6 ug/ml
  • normalized fitness= 4.2

OMP_AN:35150

OMP:0006037

increased resistance to macrolide

Relative to: OMP_AN:27519


Genotype differences:

+lacZ4787(del)( +miaA721(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Clarithromycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3732) Clarithromycin

  • chemical concentration= 1 ug/ml
  • normalized fitness= 4.13

OMP_AN:35151

OMP:0007902

increased resistance to bile acids

Relative to: OMP_AN:27586


Genotype differences:

+lacZ4787(del)( +miaA721(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Deoxycholate sodium
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9177) Deoxycholate sodium

  • chemical concentration= 2 %
  • normalized fitness= 3.23

OMP_AN:35152

OMP:0006037

increased resistance to macrolide

Relative to: OMP_AN:27520


Genotype differences:

+lacZ4787(del)( +miaA721(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Erythromycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42355) Erythromycin

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= 3.76

OMP_AN:35153

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27606


Genotype differences:

+lacZ4787(del)( +miaA721(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= 3.11

OMP_AN:35154

OMP:0007071

increased organic carbon source utilization

Relative to: OMP_AN:27616


Genotype differences:

+lacZ4787(del)( +miaA721(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= 3.11

OMP_AN:35155

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27573


Genotype differences:

+lacZ4787(del)( +miaA721(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Procaine hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8431) Procaine hydrochloride

  • chemical concentration= 10 mM
  • normalized fitness= -4.52

OMP_AN:35156

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27597


Genotype differences:

+lacZ4787(del)( +miaA721(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Radicicol
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:556075) Radicicol

  • chemical concentration= 5 uM
  • normalized fitness= 4.06

OMP_AN:35157

OMP:0007913

increased resistance to a chelator

Relative to: OMP_AN:27508


Genotype differences:

+lacZ4787(del)( +miaA721(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EGTA
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:30740) EGTA

  • chemical concentration= 1 mM
  • normalized fitness= 3.57

OMP_AN:35158

OMP:0007008

decreased population growth at acidic pH

Relative to: OMP_AN:27622


Genotype differences:

+lacZ4787(del)( +miaA721(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:MES-HOMOPIPES
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (**SID:329817955 SID:57651422) MES-HOMOPIPES

  • chemical concentration= pH 4
  • normalized fitness= -4.69


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page