OMP ST:73744 ! Escherichia coli K-12 JW3969

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 JW3969

Synonyms

JW3969-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 purD776(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:34867

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27601


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Sodium Acetate anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32954) Sodium Acetate anhydrous

  • chemical concentration= 0.6%
  • normalized fitness= -16.53

OMP_AN:34868

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27611


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Sodium Acetate anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32954) Sodium Acetate anhydrous

  • chemical concentration= 0.6%
  • normalized fitness= -16.53

OMP_AN:34869

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27575


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Acriflavine (acriflavinium chloride)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:74728) Acriflavine (acriflavinium chloride)

  • chemical concentration= 10 ug/ml
  • normalized fitness= 4.25

OMP_AN:34870

OMP:0007895

increased resistance to a surfactant

Relative to: OMP_AN:27581


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Benzalkonium chloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3020) Benzalkonium chloride

  • chemical concentration= 1 ug/ml
  • normalized fitness= 3.6

OMP_AN:34871

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27587


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:26711 + CHEBI:9177) Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)

  • chemical concentration= 0.1 %
  • normalized fitness= -10.66

OMP_AN:34872

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27549


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:CHIR-090
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:134107 CID:11546620) CHIR-090

  • chemical concentration= 0.025 ug/ml
  • normalized fitness= -10.18

OMP_AN:34873

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27586


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Deoxycholate sodium
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9177) Deoxycholate sodium

  • chemical concentration= 2 %
  • normalized fitness= -12.99

OMP_AN:34874

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27606


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= -17.52

OMP_AN:34875

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27616


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= -17.52

OMP_AN:34876

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27602


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Glucosamine hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:91431) D-(+) Glucosamine hydrochloride

  • chemical concentration= 0.2%
  • normalized fitness= -20.4

OMP_AN:34877

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27612


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Glucosamine hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:91431) D-(+) Glucosamine hydrochloride

  • chemical concentration= 0.2%
  • normalized fitness= -20.4

OMP_AN:34878

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27603


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -14.15

OMP_AN:34879

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27613


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -14.15

OMP_AN:34880

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27604


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= -16.36

OMP_AN:34881

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27614


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= -16.36

OMP_AN:34882

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27605


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= -15.89

OMP_AN:34883

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27615


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= -15.89

OMP_AN:34884

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27600


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= -19.15

OMP_AN:34885

OMP:0007032

decreased utilization of inorganic nitrogen source

Relative to: OMP_AN:27610


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= -19.15

OMP_AN:34886

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27503


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nitrofurantoin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:71415) Nitrofurantoin

  • chemical concentration= 1.5 ug/ml
  • normalized fitness= -12.54

OMP_AN:34887

OMP:0007250

increased resistance to a chemical

Relative to: OMP_AN:27570


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Propidium iodide
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:51240) Propidium iodide

  • chemical concentration= 20 ug/ml
  • normalized fitness= 3.51

OMP_AN:34888

OMP:0007896

decreased resistance to a surfactant

Relative to: OMP_AN:27582


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:SDS
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8984) SDS

  • chemical concentration= 4 %
  • normalized fitness= -3.48

OMP_AN:34889

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27607


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Succinic acid
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:15741) Succinic acid

  • chemical concentration= 0.3%
  • normalized fitness= -12.3

OMP_AN:34890

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27617


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Succinic acid
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:15741) Succinic acid

  • chemical concentration= 0.3%
  • normalized fitness= -12.3

OMP_AN:34891

OMP:0007832

increased resistance to sulfonamide

Relative to: OMP_AN:27577


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Sulfamonomethoxine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32164) Sulfamonomethoxine

  • chemical concentration= 50 ug/ml
  • normalized fitness= 3.33

OMP_AN:34892

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27560


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bacitracin Zinc Sulfate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:16219032) Bacitracin Zinc Sulfate

  • chemical concentration= 300 ug/ml
  • normalized fitness= -4.89

OMP_AN:34893

OMP:0007912

decreased resistance to a chelator

Relative to: OMP_AN:27508


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EGTA
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:30740) EGTA

  • chemical concentration= 1 mM
  • normalized fitness= -3.53

OMP_AN:34894

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27598


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= -18.58

OMP_AN:34895

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27608


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= -18.58

OMP_AN:34896

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27599


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= -18.36

OMP_AN:34897

OMP:0007937

decreased resistance to nutrient limitation

Relative to: OMP_AN:27609


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= -18.36

OMP_AN:34898

OMP:0007521

decreased population growth in high osmolarity

Relative to: OMP_AN:27595


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:NaCl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:26710) NaCl

  • chemical concentration= 150 mM
  • normalized fitness= -9.83

OMP_AN:34899

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27504


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Novobiocin sodium salt
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31924) Novobiocin sodium salt

  • chemical concentration= 8 ug/ml
  • normalized fitness= 3.44

OMP_AN:34900

OMP:0007008

decreased population growth at acidic pH

Relative to: OMP_AN:27622


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:MES-HOMOPIPES
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (**SID:329817955 SID:57651422) MES-HOMOPIPES

  • chemical concentration= 6
  • normalized fitness= -3.56

OMP_AN:34901

OMP:0007010

decreased population growth at alkaline pH

Relative to: OMP_AN:27623


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:TAPS
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (**SID:24900259) TAPS

  • chemical concentration= 9
  • normalized fitness= -3.86

OMP_AN:34902

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27565


Genotype differences:

+lacZ4787(del)( +purD776(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Polymyxin B sulfate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8310) Polymyxin B sulfate

  • chemical concentration= 6 ug/ml
  • normalized fitness= 3.79


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 2.22 2.23 2.24 2.25 2.26 2.27 2.28 2.29 2.30 2.31 2.32 2.33 2.34 2.35 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page