OMP ST:72727 ! Escherichia coli K-12 JW2324

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 JW2324

Synonyms

JW2324-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 yfcA735(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:31508

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27552


Genotype differences:

+lacZ4787(del)( +yfcA735(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azidothymidine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:10110) Azidothymidine

  • chemical concentration= 1 ng/ml
  • normalized fitness= 3.41

OMP_AN:31509

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27549


Genotype differences:

+lacZ4787(del)( +yfcA735(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:CHIR-090
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:134107 CID:11546620) CHIR-090

  • chemical concentration= 0.075 ug/ml
  • normalized fitness= -16.18

OMP_AN:31510

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27537


Genotype differences:

+lacZ4787(del)( +yfcA735(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Mecillinam
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:51208) Mecillinam

  • chemical concentration= 0.06 ug/ml
  • normalized fitness= -3.43

OMP_AN:31511

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27600


Genotype differences:

+lacZ4787(del)( +yfcA735(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= 2.99

OMP_AN:31512

OMP:0007033

increased utilization of inorganic nitrogen source

Relative to: OMP_AN:27610


Genotype differences:

+lacZ4787(del)( +yfcA735(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= 2.99

OMP_AN:31513

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27554


Genotype differences:

+lacZ4787(del)( +yfcA735(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Tunicamycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:29699) Tunicamycin

  • chemical concentration= 7.5 ug/ml
  • normalized fitness= -19.64

OMP_AN:31514

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27568


Genotype differences:

+lacZ4787(del)( +yfcA735(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:(±)-Verapamil hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:53188) (±)-Verapamil hydrochloride

  • chemical concentration= 1 mM
  • normalized fitness= -3.81

OMP_AN:31515

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27560


Genotype differences:

+lacZ4787(del)( +yfcA735(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bacitracin Zinc Sulfate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:16219032) Bacitracin Zinc Sulfate

  • chemical concentration= 200 ug/ml
  • normalized fitness= -3.83

OMP_AN:31516

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27596


Genotype differences:

+lacZ4787(del)( +yfcA735(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefaclor
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3478) Cefaclor

  • chemical concentration= 1 ug/ml
  • normalized fitness= -3.91

OMP_AN:31517

OMP:0006057

decreased resistance to aminoglycoside

Relative to: OMP_AN:27526


Genotype differences:

+lacZ4787(del)( +yfcA735(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Gentamicin sulfate salt
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:5312) Gentamicin sulfate salt

  • chemical concentration= 0.05 ug/ml
  • normalized fitness= -4.26

OMP_AN:31518

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27598


Genotype differences:

+lacZ4787(del)( +yfcA735(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= 3.53

OMP_AN:31519

OMP:0007888

increased resistance to a metal

Relative to: OMP_AN:27608


Genotype differences:

+lacZ4787(del)( +yfcA735(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= 3.53

OMP_AN:31520

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27599


Genotype differences:

+lacZ4787(del)( +yfcA735(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= 4

OMP_AN:31521

OMP:0007936

increased resistance to nutrient limitation

Relative to: OMP_AN:27609


Genotype differences:

+lacZ4787(del)( +yfcA735(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= 4

OMP_AN:31522

OMP:0007521

decreased population growth in high osmolarity

Relative to: OMP_AN:27595


Genotype differences:

+lacZ4787(del)( +yfcA735(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:NaCl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:26710) NaCl

  • chemical concentration= 600 mM
  • normalized fitness= -11.61

OMP_AN:31523

OMP:0006037

increased resistance to macrolide

Relative to: OMP_AN:27521


Genotype differences:

+lacZ4787(del)( +yfcA735(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Spiramycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:6419898) Spiramycin

  • chemical concentration= 1 ug/ml
  • normalized fitness= 4.54


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page