OMP ST:72424 ! Escherichia coli K-12 JW1854

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 JW1854

Synonyms

JW1854-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 nudB720(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:30707

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27579


Genotype differences:

+lacZ4787(del)( +nudB720(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Copper stress-CuCl2 dihydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:86318) Copper stress-CuCl2 dihydrate

  • chemical concentration= 4 mM (LB)
  • normalized fitness= -3.42

OMP_AN:30708

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27606


Genotype differences:

+lacZ4787(del)( +nudB720(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= -3.41

OMP_AN:30709

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27616


Genotype differences:

+lacZ4787(del)( +nudB720(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= -3.41

OMP_AN:30710

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27602


Genotype differences:

+lacZ4787(del)( +nudB720(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Glucosamine hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:91431) D-(+) Glucosamine hydrochloride

  • chemical concentration= 0.2%
  • normalized fitness= -4.04

OMP_AN:30711

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27612


Genotype differences:

+lacZ4787(del)( +nudB720(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Glucosamine hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:91431) D-(+) Glucosamine hydrochloride

  • chemical concentration= 0.2%
  • normalized fitness= -4.04

OMP_AN:30712

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27603


Genotype differences:

+lacZ4787(del)( +nudB720(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -6.32

OMP_AN:30713

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27613


Genotype differences:

+lacZ4787(del)( +nudB720(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -6.32

OMP_AN:30714

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27604


Genotype differences:

+lacZ4787(del)( +nudB720(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= -3.74

OMP_AN:30715

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27614


Genotype differences:

+lacZ4787(del)( +nudB720(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= -3.74

OMP_AN:30716

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27605


Genotype differences:

+lacZ4787(del)( +nudB720(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= -5.17

OMP_AN:30717

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27615


Genotype differences:

+lacZ4787(del)( +nudB720(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= -5.17

OMP_AN:30718

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27554


Genotype differences:

+lacZ4787(del)( +nudB720(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Tunicamycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:29699) Tunicamycin

  • chemical concentration= 1 ug/ml
  • normalized fitness= 3.56

OMP_AN:30719

OMP:0007913

increased resistance to a chelator

Relative to: OMP_AN:27508


Genotype differences:

+lacZ4787(del)( +nudB720(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EGTA
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:30740) EGTA

  • chemical concentration= 2 mM
  • normalized fitness= 3.26

OMP_AN:30720

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27591


Genotype differences:

+lacZ4787(del)( +nudB720(del) -lacZ4787(del)


Condition differences:

no differences

ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:45924 + CHEBI:9331) Trimethoprim (CHEBI:45924) + Sulfamethizole (CHEBI:9331)

  • chemical concentration= 0.1 + 50 ug/ml
  • normalized fitness= -3.26


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page