OMP ST:71585 ! Escherichia coli K-12 JW0580

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 JW0580

Synonyms

JW0580-5

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 fepC726(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes


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Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:28756

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27601

Genotype differences:

+lacZ4787(del)( +fepC726(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Sodium Acetate anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32954) Sodium Acetate anhydrous

  • chemical concentration= 0.6%
  • normalized fitness= -12.8

OMP_AN:28757

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27611

Genotype differences:

+lacZ4787(del)( +fepC726(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Sodium Acetate anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:32954) Sodium Acetate anhydrous

  • chemical concentration= 0.6%
  • normalized fitness= -12.8

OMP_AN:28758

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27531

Genotype differences:

+lacZ4787(del)( +fepC726(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Ampicillin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28971) Ampicillin

  • chemical concentration= 8 ug/ml
  • normalized fitness= -6.94

OMP_AN:28759

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27549

Genotype differences:

+lacZ4787(del)( +fepC726(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:CHIR-090
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:134107 CID:11546620) CHIR-090

  • chemical concentration= 0.025 ug/ml
  • normalized fitness= -4.49

OMP_AN:28760

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27606

Genotype differences:

+lacZ4787(del)( +fepC726(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= -5.25

OMP_AN:28761

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27616

Genotype differences:

+lacZ4787(del)( +fepC726(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= -5.25

OMP_AN:28762

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27602

Genotype differences:

+lacZ4787(del)( +fepC726(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Glucosamine hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:91431) D-(+) Glucosamine hydrochloride

  • chemical concentration= 0.2%
  • normalized fitness= -5.09

OMP_AN:28763

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27612

Genotype differences:

+lacZ4787(del)( +fepC726(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Glucosamine hydrochloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:91431) D-(+) Glucosamine hydrochloride

  • chemical concentration= 0.2%
  • normalized fitness= -5.09

OMP_AN:28764

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27603

Genotype differences:

+lacZ4787(del)( +fepC726(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -6.07

OMP_AN:28765

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27613

Genotype differences:

+lacZ4787(del)( +fepC726(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -6.07

OMP_AN:28766

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27604

Genotype differences:

+lacZ4787(del)( +fepC726(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= -5.72

OMP_AN:28767

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27614

Genotype differences:

+lacZ4787(del)( +fepC726(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17754) Glycerol

  • chemical concentration= 0.4%
  • normalized fitness= -5.72

OMP_AN:28768

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27605

Genotype differences:

+lacZ4787(del)( +fepC726(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= -5.23

OMP_AN:28769

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27615

Genotype differences:

+lacZ4787(del)( +fepC726(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= -5.23

OMP_AN:28770

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27537

Genotype differences:

+lacZ4787(del)( +fepC726(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Mecillinam
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:51208) Mecillinam

  • chemical concentration= 0.03 ug/ml
  • normalized fitness= -3.35

OMP_AN:28771

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27593

Genotype differences:

+lacZ4787(del)( +fepC726(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:(-)-Norepinephrine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:18357) (-)-Norepinephrine

  • chemical concentration= 100 uM
  • normalized fitness= -3.84

OMP_AN:28772

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27607

Genotype differences:

+lacZ4787(del)( +fepC726(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Succinic acid
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:15741) Succinic acid

  • chemical concentration= 0.3%
  • normalized fitness= -8.54

OMP_AN:28773

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27617

Genotype differences:

+lacZ4787(del)( +fepC726(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Succinic acid
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:15741) Succinic acid

  • chemical concentration= 0.3%
  • normalized fitness= -8.54

OMP_AN:28774

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27560

Genotype differences:

+lacZ4787(del)( +fepC726(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bacitracin Zinc Sulfate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:16219032) Bacitracin Zinc Sulfate

  • chemical concentration= 300 ug/ml
  • normalized fitness= -3.42

OMP_AN:28775

OMP:0007008

decreased population growth at acidic pH

Relative to: OMP_AN:27622

Genotype differences:

+lacZ4787(del)( +fepC726(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:MES-HOMOPIPES
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (**SID:329817955 SID:57651422) MES-HOMOPIPES

  • chemical concentration= 6
  • normalized fitness= -4.31
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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