OMP ST:71354 ! Escherichia coli K-12 JW0233

From OMPwiki
Revision as of 19:22, 7 June 2021 by Siegele (talk | contribs) (Table edited by $2 via TableEdit)

Strain Summary

Strain Name

Escherichia coli K-12 JW0233

Synonyms

JW0233-2

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)::rrnB-3 rph-1 proA761(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:28211

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27601


Genotype differences:

+proA761(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium: Sodium Acetate (0.6%)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

normalized fitness= -9.5

OMP_AN:28213

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27606


Genotype differences:

+proA761(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine (0.15%)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

normalized fitness= -15.59

OMP_AN:28215

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27602


Genotype differences:

+proA761(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Glucosamine hydrochloride (0.2%)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

  • (CID:91431) D-(+) Glucosamine hydrochloride= 0.2%
  • normalized fitness= -15.4

OMP_AN:28217

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27603


Genotype differences:

+proA761(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose (0.2%)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -16.79

OMP_AN:28219

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27604


Genotype differences:

+proA761(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol (0.4%)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

normalized fitness= -20.78

OMP_AN:28221

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27605


Genotype differences:

+proA761(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose (0.1%)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

normalized fitness= -15.46

OMP_AN:28223

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27600


Genotype differences:

+proA761(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl (9.5 mM)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

normalized fitness= -23.94

OMP_AN:28225

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27607


Genotype differences:

+proA761(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Succinic acid (0.3%)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

normalized fitness= -10.5

OMP_AN:28226

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27617


Genotype differences:

+proA761(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Succinic acid
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:15741) Succinic acid

  • chemical concentration= 0.3%
  • normalized fitness= -10.5

OMP_AN:28227

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27598


Genotype differences:

+proA761(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:FeSO4 (1 mM, iron excess)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= -24.76

OMP_AN:28228

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27608


Genotype differences:

+proA761(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= -24.76

OMP_AN:28229

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27599


Genotype differences:

+proA761(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= -13.42

OMP_AN:28230

OMP:0007937

decreased resistance to nutrient limitation

Relative to: OMP_AN:27609


Genotype differences:

+proA761(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= -13.42


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page