Difference between revisions of "OMP ST:71354 ! Escherichia coli K-12 JW0233"

From OMPwiki
(Table edited by $2 via TableEdit)
(Table edited by $2 via TableEdit)
 
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*chemical concentration= 1mM (MOPS; normal 100uM)
 
*normalized fitness= -24.76
 
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OMP_AN:28228
 
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OMP:0007886
 
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decreased resistance to a metal
 
|
 
Relative to: OMP_AN:27608
 
 
<hr>Genotype differences:<br>
 
+proA761(del)
 
<hr>Condition differences:<br>
 
no differences
 
|
 
 
*temperature:37C
 
*medium:MOPS
 
*medium:Iron excess-FeSO4 heptahydride
 
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ECO:0007005
 
|
 
high throughput direct assay evidence used in manual assertion
 
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PMID:21185072
 
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towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride
 
 
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Line 424: Line 391:
  
 
|
 
|
OMP:0007075
+
OMP:0007074
 
|
 
|
decreased growth factor requirement
+
increased growth factor requirement
 
|
 
|
 
Relative to: OMP_AN:27599
 
Relative to: OMP_AN:27599
Line 438: Line 405:
 
*temperature:37C
 
*temperature:37C
 
*medium:MOPS
 
*medium:MOPS
*medium:Iron starvation-FeSO4·7H2O
+
*medium:FeSO4 (1 uM, Iron starvation)
 
|
 
|
 
ECO:0007005
 
ECO:0007005
Line 446: Line 413:
 
PMID:21185072
 
PMID:21185072
 
|
 
|
towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O
 
|
 
 
*chemical concentration= 2uM (MOPS; normal 100uM)
 
*normalized fitness= -13.42
 
|-
 
|
 
OMP_AN:28230
 
|
 
 
|
 
OMP:0007937
 
|
 
decreased resistance to nutrient limitation
 
|
 
Relative to: OMP_AN:27609
 
  
<hr>Genotype differences:<br>
 
+proA761(del)
 
<hr>Condition differences:<br>
 
no differences
 
|
 
 
*temperature:37C
 
*medium:MOPS
 
*medium:Iron starvation-FeSO4·7H2O
 
|
 
ECO:0007005
 
|
 
high throughput direct assay evidence used in manual assertion
 
|
 
PMID:21185072
 
|
 
towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O
 
 
|
 
|
  
*chemical concentration= 2uM (MOPS; normal 100uM)
+
*FeSO4 concentration= 2uM (MOPS; normal 100uM)
 
*normalized fitness= -13.42  
 
*normalized fitness= -13.42  
  

Latest revision as of 19:24, 7 June 2021

Strain Summary

Strain Name

Escherichia coli K-12 JW0233

Synonyms

JW0233-2

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)::rrnB-3 rph-1 proA761(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:28211

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27601


Genotype differences:

+proA761(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium: Sodium Acetate (0.6%)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

normalized fitness= -9.5

OMP_AN:28213

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27606


Genotype differences:

+proA761(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine (0.15%)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

normalized fitness= -15.59

OMP_AN:28215

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27602


Genotype differences:

+proA761(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Glucosamine hydrochloride (0.2%)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

  • (CID:91431) D-(+) Glucosamine hydrochloride= 0.2%
  • normalized fitness= -15.4

OMP_AN:28217

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27603


Genotype differences:

+proA761(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose (0.2%)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -16.79

OMP_AN:28219

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27604


Genotype differences:

+proA761(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Glycerol (0.4%)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

normalized fitness= -20.78

OMP_AN:28221

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27605


Genotype differences:

+proA761(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose (0.1%)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

normalized fitness= -15.46

OMP_AN:28223

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27600


Genotype differences:

+proA761(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl (9.5 mM)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

normalized fitness= -23.94

OMP_AN:28225

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27607


Genotype differences:

+proA761(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Succinic acid (0.3%)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

normalized fitness= -10.5

OMP_AN:28226

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27617


Genotype differences:

+proA761(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:Succinic acid
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:15741) Succinic acid

  • chemical concentration= 0.3%
  • normalized fitness= -10.5

OMP_AN:28227

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27598


Genotype differences:

+proA761(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:FeSO4 (1 mM, iron excess)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= -24.76

OMP_AN:28229

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27599


Genotype differences:

+proA761(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:FeSO4 (1 uM, Iron starvation)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

  • FeSO4 concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= -13.42


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page