OMP ST:71245 ! Escherichia coli K-12 JW0052

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 JW0052

Synonyms

JW0052-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 surA765(del)::FRT-kan-FRT

Strain Reference
Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:27863

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27575


Genotype differences:

+lacZ4787(del)( +surA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Acriflavine (acriflavinium chloride)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:74728) Acriflavine (acriflavinium chloride)

  • chemical concentration= 2 ug/ml
  • normalized fitness= -5.56

OMP_AN:27864

OMP:0007927

decreased anaerobic population growth

Relative to: OMP_AN:27618


Genotype differences:

+lacZ4787(del)( +surA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Anaerobic
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

  • normalized fitness= -4.8

OMP_AN:27865

OMP:0006036

decreased resistance to macrolide

Relative to: OMP_AN:27518


Genotype differences:

+lacZ4787(del)( +surA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azithromycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:2955) Azithromycin

  • chemical concentration= 1 ug/ml
  • normalized fitness= -6.5

OMP_AN:27866

OMP:0007173

decreased resistance to host defense peptides

Relative to: OMP_AN:27562


Genotype differences:

+lacZ4787(del)( +surA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cecropin B
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:16130488) Cecropin B

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= -4.88

OMP_AN:27867

OMP:0006037

increased resistance to macrolide

Relative to: OMP_AN:27519


Genotype differences:

+lacZ4787(del)( +surA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Clarithromycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3732) Clarithromycin

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= 3.92

OMP_AN:27868

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27510


Genotype differences:

+lacZ4787(del)( +surA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Mitomycin C
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:27504) Mitomycin C

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= -8.77

OMP_AN:27869

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27553


Genotype differences:

+lacZ4787(del)( +surA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Puromycin dihydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8642) Puromycin dihydrochloride

  • chemical concentration= 5 ug/ml
  • normalized fitness= -4.5

OMP_AN:27870

OMP:0007896

decreased resistance to a surfactant

Relative to: OMP_AN:27582


Genotype differences:

+lacZ4787(del)( +surA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:SDS
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8984) SDS

  • chemical concentration= 0.5 %
  • normalized fitness= -11.66

OMP_AN:27871

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27584


Genotype differences:

+lacZ4787(del)( +surA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Taurocholic acid sodium salt hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23687511) Taurocholic acid sodium salt hydrate

  • chemical concentration= 0.5 %
  • normalized fitness= -4.6

OMP_AN:27872

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27567


Genotype differences:

+lacZ4787(del)( +surA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Theophylline anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28177) Theophylline anhydrous

  • chemical concentration= 100 ug/ml
  • normalized fitness= -5.65

OMP_AN:27873

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27588


Genotype differences:

+lacZ4787(del)( +surA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Triclosan/Irgasan
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:164200) Triclosan/Irgasan

  • chemical concentration= 0.05 ug/ml
  • normalized fitness= -3.71

OMP_AN:27874

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27560


Genotype differences:

+lacZ4787(del)( +surA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bacitracin Zinc Sulfate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:16219032) Bacitracin Zinc Sulfate

  • chemical concentration= 300 ug/ml
  • normalized fitness= -8.89

OMP_AN:27875

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27504


Genotype differences:

+lacZ4787(del)( +surA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Novobiocin sodium salt
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31924) Novobiocin sodium salt

  • chemical concentration= 8 ug/ml
  • normalized fitness= -4.58

OMP_AN:27876

OMP:0007008

decreased population growth at acidic pH

Relative to: OMP_AN:27622


Genotype differences:

+lacZ4787(del)( +surA765(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:MES-HOMOPIPES
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (**SID:329817955 SID:57651422) MES-HOMOPIPES

  • chemical concentration= pH 4
  • normalized fitness= -14.8

OMP_AN:27877

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27591


Genotype differences:

+lacZ4787(del)( +surA765(del) -lacZ4787(del)


Condition differences:

no differences

ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:45924 + CHEBI:9331) Trimethoprim (CHEBI:45924) + Sulfamethizole (CHEBI:9331)

  • chemical concentration= 0.1 + 50 ug/ml
  • normalized fitness= -4.36


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed OMPwiki page
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page