Difference between revisions of "OMP ST:71063 ! Escherichia coli K-12 BW25113 prfA::SPA-kan"

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*chemical concentration= 3 uM
 
*chemical concentration= 3 uM
 
*normalized fitness= -4.61  
 
*normalized fitness= -4.61  
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OMP_AN:37521
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OMP:0006036
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decreased resistance to macrolide
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Relative to: OMP_AN:27520
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<hr>Genotype differences:<br>
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+lacZ4787(del)( +prfA  -lacZ4787(del)
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<hr>Condition differences:<br>
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no differences
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*temperature:37C
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*medium:LB agar
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*medium:Erythromycin
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:42355) Erythromycin
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*chemical concentration=  10 ug/ml
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*normalized fitness= -3.38
  
 
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Revision as of 13:06, 23 October 2019

Strain Summary

Strain Name

Escherichia coli K-12 BW25113 prfA::SPA-kan

Synonyms
Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 prfA::SPA-kan

Strain Reference

PMID:21185072[1]

Strain availability
Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:37518

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27530


Genotype differences:

+lacZ4787(del)( +prfA -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Amoxicillin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:2676) Amoxicillin

  • chemical concentration= 1 ug/ml
  • normalized fitness= -4.25

OMP_AN:37519

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27535


Genotype differences:

+lacZ4787(del)( +prfA -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefsulodin sodium salt hydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:31380) Cefsulodin sodium salt hydrate

  • chemical concentration= 6 ug/ml
  • normalized fitness= -3.87

OMP_AN:37520

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27514


Genotype differences:

+lacZ4787(del)( +prfA -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Chlorpromazine hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:3649) Chlorpromazine hydrochloride

  • chemical concentration= 3 uM
  • normalized fitness= -4.61

OMP_AN:37521

OMP:0006036

decreased resistance to macrolide

Relative to: OMP_AN:27520


Genotype differences:

+lacZ4787(del)( +prfA -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Erythromycin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:42355) Erythromycin

  • chemical concentration= 10 ug/ml
  • normalized fitness= -3.38


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. 1.0 1.1 1.2 1.3 1.4 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page