OMP ST:71037 ! Escherichia coli K-12 BW25113 ispA::SPA-kan

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 BW25113 ispA::SPA-kan

Synonyms
Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 ispA::SPA-kan

Strain Reference

PMID:21185072[1]

Strain availability
Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:37353

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27559


Genotype differences:

+lacZ4787(del)( +ispA -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Actinomycin D
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:27666 CID:457193) Actinomycin D

  • chemical concentration= 10 ug/ml
  • normalized fitness= -3.82

OMP_AN:37354

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27587


Genotype differences:

+lacZ4787(del)( +ispA -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:26711 + CHEBI:9177) Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)

  • chemical concentration= 2 %
  • normalized fitness= -3.38

OMP_AN:37355

OMP:0006040

decreased resistance to quinolone

Relative to: OMP_AN:27543


Genotype differences:

+lacZ4787(del)( +ispA -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Streptonigrin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:9287) Streptonigrin

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= -4.23

OMP_AN:37356

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27565


Genotype differences:

+lacZ4787(del)( +ispA -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Polymyxin B sulfate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:8310) Polymyxin B sulfate

  • chemical concentration= 4 ug/ml
  • normalized fitness= -4.56

OMP_AN:37357

OMP:0006057

decreased resistance to aminoglycoside

Relative to: OMP_AN:27527


Genotype differences:

+lacZ4787(del)( +ispA -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Spectinomycin dihydrochloride pentahydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[1]

towards some (CHEBI:9217) Spectinomycin dihydrochloride pentahydrate

  • chemical concentration= 6 ug/ml
  • normalized fitness= -3.75


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page