OMP ST:71006 ! Escherichia coli K-12 BW25113 murE+-kan

Strain Summary

Strain Name	Escherichia coli K-12 BW25113 murE+-kan
Synonyms	ECK0086-A in Supplemental Table S2
Taxon Information	Pangenome: Escherichia coli Subspecies and/or strain: K-12 NCBI Taxonomy ID: 83333
Genotype	DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 murE+-kan
Strain Reference	PMID:21185072
Strain availability
Ancestry	parent:OMP ST:800 ! Escherichia coli K-12 BW25113
Annotated phenotypes
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Phenotype Annotation

Annotation ID	Qualifier	OMP ID	OMP term name	Relative phenotype information	Experimental condition	ECO ID	ECO term name	Reference	Annotation Extension	Notes
OMP_AN:37470		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27594 Genotype differences: +lacZ4787(del)( +murE+-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:SDS (CHEBI:8984) +EDTA (CHEBI:64758)	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:64758 + CHEBI:8984) SDS (CHEBI:8984) +EDTA (CHEBI:64758)	chemical concentration= 0.5%/0.5mM %/0.5mM normalized fitness= -3.84
OMP_AN:37471		OMP:0007878	increased resistance to an antimicrobial agent	Relative to: OMP_AN:27552 Genotype differences: +lacZ4787(del)( +murE+-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Azidothymidine	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:10110) Azidothymidine	chemical concentration= 2.5 ng/ml normalized fitness= 3.47
OMP_AN:37472		OMP:0007896	decreased resistance to a surfactant	Relative to: OMP_AN:27581 Genotype differences: +lacZ4787(del)( +murE+-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Benzalkonium chloride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:3020) Benzalkonium chloride	chemical concentration= 10 ug/ml normalized fitness= -3.61
OMP_AN:37473		OMP:0006036	decreased resistance to macrolide	Relative to: OMP_AN:27519 Genotype differences: +lacZ4787(del)( +murE+-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Clarithromycin	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:3732) Clarithromycin	chemical concentration= 10 ug/ml normalized fitness= -3.83
OMP_AN:37474		OMP:0007833	decreased resistance to sulfonamde	Relative to: OMP_AN:27577 Genotype differences: +lacZ4787(del)( +murE+-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Sulfamonomethoxine	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:32164) Sulfamonomethoxine	chemical concentration= 50 ug/ml normalized fitness= -4.45
OMP_AN:37475		OMP:0007075	decreased growth factor requirement	Relative to: OMP_AN:27599 Genotype differences: +lacZ4787(del)( +murE+-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:MOPS medium:Iron starvation-FeSO4·7H2O	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O	chemical concentration= 2uM (MOPS; normal 100uM) normalized fitness= -3.45
OMP_AN:37476		OMP:0007937	decreased resistance to nutrient limitation	Relative to: OMP_AN:27609 Genotype differences: +lacZ4787(del)( +murE+-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:MOPS medium:Iron starvation-FeSO4·7H2O	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O	chemical concentration= 2uM (MOPS; normal 100uM) normalized fitness= -3.45
OMP_AN:37477		OMP:0007833	decreased resistance to sulfonamde	Relative to: OMP_AN:27576 Genotype differences: +lacZ4787(del)( +murE+-kan -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Sulfamethizole	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:9331) Sulfamethizole	chemical concentration= 100 ug/ml normalized fitness= -4.51
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References

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