Difference between revisions of "OMP ST:6329 ! Escherichia coli O157:H7 EDL933"

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(Table edited by Kcunningham via TableEdit)
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OMP_AN:7627
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OMP:0000176
 +
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biofilm phenotype
 +
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 +
Relative to:
 +
 +
<hr>Genotype differences:<br>
 +
Nothing to compare<br/>
 +
<hr>Condition differences:<br>
 +
Nothing to compare<br/>
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*other:96-well plate
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*temperature:37C
 +
*medium:LB medium
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ECO:0000181
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in vitro assay evidence
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PMID:22047137
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Crystal violet biofilm assay
  
 
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Revision as of 15:57, 7 December 2016

Strain Summary

Strain Name

Escherichia coli O157:H7 EDL933

Synonyms
Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: O157:H7
  • NCBI Taxonomy ID: 83334
Genotype
Strain Reference
Strain availability

ATCC 43895

Ancestry
Annotated phenotypes




Notes

Phenotype Annotation

Annotation ID not OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:7573

OMP:0000176

biofilm phenotype

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • other:96 well plate
  • temperature:37C
  • medium:LB medium
ECO:0000181

in vitro assay evidence

PMID:24837471[1]

Biofilm formation assay

OMP_AN:7574

OMP:0007026

decreased biofilm formation

Relative to: OMP_AN:7573


Genotype differences:

no differences


Condition differences:

medium:>= Coladonin (0.5 ug/mL)?

  • other:96 well plate
  • temperature:37C
  • medium:>= Coladonin (0.5 ug/mL)
  • medium:LB medium
ECO:0000181

in vitro assay evidence

PMID:24837471[1]

Biofilm formation assay

OMP_AN:7575

OMP:0007026

decreased biofilm formation

Relative to: OMP_AN:7573


Genotype differences:

no differences


Condition differences:

medium:>= Coumarin (0.5 ug./mL)?

  • other:96 well plate
  • temperature:37C
  • medium:>= Coumarin (0.5 ug./mL)
  • medium:LB medium
ECO:0000181

in vitro assay evidence

PMID:24837471[1]

CHEBI:28794 Coumarin

Biofilm formation assay

OMP_AN:7576

OMP:0007026

decreased biofilm formation

Relative to: OMP_AN:7573


Genotype differences:

no differences


Condition differences:

medium:>= Umbelliferone (0.5 ug/mL)?

  • other:96 well plate
  • temperature:37C
  • medium:>= Umbelliferone (0.5 ug/mL)
  • medium:LB medium
ECO:0000181

in vitro assay evidence

PMID:24837471[1]

CHEBI:27510 Umbelliferone

Biofilm formation assay

OMP_AN:7577

OMP:0000067

presence of cell motility by swarming

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • temperature:37C
  • medium:swarming motility medium
ECO:0005803

motility assay evidence

PMID:24837471[1]

OMP_AN:7578

OMP:0007092

decreased cell motility by swarming

Relative to: OMP_AN:7577


Genotype differences:

no differences


Condition differences:

medium:Coumarin (50 ug/mL)?

  • temperature:37C
  • medium:Coumarin (50 ug/mL)
  • medium:swarming motility medium
ECO:0005803

motility assay evidence

PMID:24837471[1]

CHEBI:28794 Coumarin

OMP_AN:7579

OMP:0007092

decreased cell motility by swarming

Relative to: OMP_AN:7577


Genotype differences:

no differences


Condition differences:

medium:Umbelliferone (50 ug/mL)?

  • temperature:37C
  • medium:Umbelliferone (50 ug/mL)
  • medium:swarming motility medium
ECO:0005803

motility assay evidence

PMID:24837471[1]

CHEBI:27510 Umbelliferone

OMP_AN:7580

OMP:0000111

presence of cell motility by swimming

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • temperature:37C
  • medium:swimming motility medium
ECO:0005803

motility assay evidence

PMID:24837471[1]

OMP_AN:7581

NOT

OMP:0005324

altered cell motility by swimming

Relative to: OMP_AN:7580


Genotype differences:

no differences


Condition differences:

medium:Coumarin (50 ug/mL)?

  • temperature:37C
  • medium:Coumarin (50 ug/mL)
  • medium:swimming motility medium
ECO:0005803

motility assay evidence

PMID:24837471[1]

CHEBI:28794 Coumarin

OMP_AN:7582

NOT

OMP:0005324

altered cell motility by swimming

Relative to: OMP_AN:7580


Genotype differences:

no differences


Condition differences:

medium:Umbelliferone (50 ug/mL)?

  • temperature:37C
  • medium:Umbelliferone (50 ug/mL)
  • medium:swimming motility medium
ECO:0005803

motility assay evidence

PMID:24837471[1]

CHEBI:27510 Umbelliferone

OMP_AN:7583

OMP:0000084

pilus phenotype

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • other:nylon filter
  • temperature:37C
  • medium:LB medium
ECO:0005033

electron microscopy evidence

PMID:24837471[1]

OMP_AN:7584

OMP:0000028

decreased number of pili

Relative to: OMP_AN:7583


Genotype differences:

no differences


Condition differences:

medium:Coumarin (50 ug/mL)?

  • other:nylon filter
  • temperature:37C
  • medium:Coumarin (50 ug/mL)
  • medium:LB medium
ECO:0005033

electron microscopy evidence

PMID:24837471[1]

CHEBI:28794 Coumarin

OMP_AN:7585

OMP:0000028

decreased number of pili

Relative to: OMP_AN:7583


Genotype differences:

no differences


Condition differences:

medium:Umbelliferone (50 ug/mL)?

  • other:nylon filter
  • temperature:37C
  • medium:LB medium
  • medium:Umbelliferone (50 ug/mL)
ECO:0005033

electron microscopy evidence

PMID:24837471[1]

CHEBI:27510 Umbelliferone

OMP_AN:7591

OMP:0000253

gene expression phenotype

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • temperature:37C
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:Q93U24 csgA

OMP_AN:7592

OMP:0000253

gene expression phenotype

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • temperature:37C
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:P0ABK8 csgB

OMP_AN:7593

OMP:0000253

gene expression phenotype

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • temperature:37C
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:Q8XCE8 flhD

OMP_AN:7594

OMP:0000253

gene expression phenotype

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • temperature:37C
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:P0AF07 motB

OMP_AN:7595

OMP:0000253

gene expression phenotype

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • temperature:37C
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:Q7DI68 stx2

OMP_AN:7596

OMP:0000253

gene expression phenotype

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • temperature:37C
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:Q8XAY7 lsrA

OMP_AN:7597

OMP:0000253

gene expression phenotype

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • temperature:37C
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:Q8X902 luxS

OMP_AN:7598

OMP:0000253

gene expression phenotype

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • temperature:37C
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

rrsG

OMP_AN:7599

OMP:0000253

gene expression phenotype

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • temperature:37C
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:Q7DH26 stx1

OMP_AN:7600

OMP:0000268

decreased gene expression

Relative to: OMP_AN:7591


Genotype differences:

no differences


Condition differences:

medium:Coumarin (50 ug/mL)?

  • temperature:37C
  • medium:Coumarin (50 ug/mL)
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:Q93U24 csgA CHEBI:28794 Coumarin

OMP_AN:7601

OMP:0000268

decreased gene expression

Relative to: OMP_AN:7591


Genotype differences:

no differences


Condition differences:

medium:Umbelliferone (50 ug/mL)?

  • temperature:37C
  • medium:LB medium
  • medium:Umbelliferone (50 ug/mL)
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:Q93U24 csgA CHEBI:27510 Umbelliferone

OMP_AN:7602

OMP:0000268

decreased gene expression

Relative to: OMP_AN:7591


Genotype differences:

no differences


Condition differences:

medium:Esculetin (50 ug/mL)?

  • temperature:37C
  • medium:Esculetin (50 ug/mL)
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:Q93U24 csgA CHEBI:490095 Esculetin

OMP_AN:7603

OMP:0000268

decreased gene expression

Relative to: OMP_AN:7592


Genotype differences:

no differences


Condition differences:

medium:Coumarin (50 ug/mL)?

  • temperature:37C
  • medium:Coumarin (50 ug/mL)
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:P0ABK8 csgB CHEBI:28794 Coumarin

OMP_AN:7604

OMP:0000268

decreased gene expression

Relative to: OMP_AN:7592


Genotype differences:

no differences


Condition differences:

medium:Umbelliferone (50 ug/mL)?

  • temperature:37C
  • medium:LB medium
  • medium:Umbelliferone (50 ug/mL)
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:P0ABK8 csgB CHEBI:27510 Umbelliferone

OMP_AN:7605

OMP:0000268

decreased gene expression

Relative to: OMP_AN:7592


Genotype differences:

no differences


Condition differences:

medium:Esculetin (50 ug/mL)?

  • temperature:37C
  • medium:Esculetin (50 ug/mL)
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:P0ABK8 csgB CHEBI:490095 Esculetin

OMP_AN:7606

OMP:0000268

decreased gene expression

Relative to: OMP_AN:7593


Genotype differences:

no differences


Condition differences:

medium:Coumarin (50 ug/mL)?

  • temperature:37C
  • medium:Coumarin (50 ug/mL)
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:Q8XCE8 flhD CHEBI:28794 Coumarin

OMP_AN:7607

OMP:0000268

decreased gene expression

Relative to: OMP_AN:7593


Genotype differences:

no differences


Condition differences:

medium:Umbelliferone (50 ug/mL)?

  • temperature:37C
  • medium:LB medium
  • medium:Umbelliferone (50 ug/mL)
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:Q8XCE8 flhD CHEBI:27510 Umbelliferone

OMP_AN:7608

OMP:0000268

decreased gene expression

Relative to: OMP_AN:7593


Genotype differences:

no differences


Condition differences:

medium:Esculetin (50 ug/mL)?

  • temperature:37C
  • medium:Esculetin (50 ug/mL)
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:Q8XCE8 flhD CHEBI:490095 Esculetin

OMP_AN:7609

OMP:0000268

decreased gene expression

Relative to: OMP_AN:7594


Genotype differences:

no differences


Condition differences:

medium:Coumarin (50 ug/mL)?

  • temperature:37C
  • medium:Coumarin (50 ug/mL)
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:P0AF07 motB CHEBI:28794 Coumarin

OMP_AN:7610

OMP:0000268

decreased gene expression

Relative to: OMP_AN:7594


Genotype differences:

no differences


Condition differences:

medium:Umbelliferone (50 ug/mL)?

  • temperature:37C
  • medium:LB medium
  • medium:Umbelliferone (50 ug/mL)
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:P0AF07 motB CHEBI:27510 Umbelliferone

OMP_AN:7611

OMP:0000268

decreased gene expression

Relative to: OMP_AN:7594


Genotype differences:

no differences


Condition differences:

medium:Esculetin (50 ug/mL)?

  • temperature:37C
  • medium:Esculetin (50 ug/mL)
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:P0AF07 motB CHEBI:490095 Esculetin

OMP_AN:7612

OMP:0000268

decreased gene expression

Relative to: OMP_AN:7595


Genotype differences:

no differences


Condition differences:

medium:Esculetin (50 ug/mL)?

  • temperature:37C
  • medium:Esculetin (50 ug/mL)
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:Q7DI68 stx2 CHEBI:490095 Esculetin

OMP_AN:7613

OMP:0000268

decreased gene expression

Relative to: OMP_AN:7596


Genotype differences:

no differences


Condition differences:

medium:Coumarin (50 ug/mL)?

  • temperature:37C
  • medium:Coumarin (50 ug/mL)
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:Q8XAY7 lsrA CHEBI:28794 Coumarin

OMP_AN:7614

OMP:0000268

decreased gene expression

Relative to: OMP_AN:7596


Genotype differences:

no differences


Condition differences:

medium:Umbelliferone (50 ug/mL)?

  • temperature:37C
  • medium:LB medium
  • medium:Umbelliferone (50 ug/mL)
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:Q8XAY7 lsrA CHEBI:27510 Umbelliferone

OMP_AN:7615

OMP:0000267

increased gene expression

Relative to: OMP_AN:7595


Genotype differences:

no differences


Condition differences:

medium:Coumarin (50 ug/mL)?

  • temperature:37C
  • medium:Coumarin (50 ug/mL)
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:Q7DI68 stx2 CHEBI:28794 Coumarin

OMP_AN:7616

OMP:0000267

increased gene expression

Relative to: OMP_AN:7595


Genotype differences:

no differences


Condition differences:

medium:Umbelliferone (50 ug/mL)?

  • temperature:37C
  • medium:LB medium
  • medium:Umbelliferone (50 ug/mL)
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:Q7DI68 stx2 CHEBI:27510 Umbelliferone

OMP_AN:7617

OMP:0000267

increased gene expression

Relative to: OMP_AN:7596


Genotype differences:

no differences


Condition differences:

medium:Esculetin (50 ug/mL)?

  • temperature:37C
  • medium:Esculetin (50 ug/mL)
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:Q8XAY7 lsrA CHEBI:490095 Esculetin

OMP_AN:7618

OMP:0000267

increased gene expression

Relative to: OMP_AN:7597


Genotype differences:

no differences


Condition differences:

medium:Coumarin (50 ug/mL)?

  • temperature:37C
  • medium:Coumarin (50 ug/mL)
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:Q8X902 luxS CHEBI:28794 Coumarin

OMP_AN:7619

OMP:0000267

increased gene expression

Relative to: OMP_AN:7597


Genotype differences:

no differences


Condition differences:

medium:Umbelliferone (50 ug/mL)?

  • temperature:37C
  • medium:LB medium
  • medium:Umbelliferone (50 ug/mL)
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:Q8X902 luxS CHEBI:27510 Umbelliferone

OMP_AN:7620

OMP:0000267

increased gene expression

Relative to: OMP_AN:7597


Genotype differences:

no differences


Condition differences:

medium:Esculetin (50 ug/mL)?

  • temperature:37C
  • medium:Esculetin (50 ug/mL)
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:Q8X902 luxS CHEBI:490095 Esculetin

OMP_AN:7621

OMP:0000267

increased gene expression

Relative to: OMP_AN:7598


Genotype differences:

no differences


Condition differences:

medium:Coumarin (50 ug/mL)?

  • temperature:37C
  • medium:Coumarin (50 ug/mL)
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

rrsG CHEBI:28794 Coumarin

OMP_AN:7622

OMP:0000267

increased gene expression

Relative to: OMP_AN:7598


Genotype differences:

no differences


Condition differences:

medium:Umbelliferone (50 ug/mL)?

  • temperature:37C
  • medium:LB medium
  • medium:Umbelliferone (50 ug/mL)
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

rrsG CHEBI:27510 Umbelliferone

OMP_AN:7623

NOT

OMP:0007080

altered gene expression

Relative to: OMP_AN:7598


Genotype differences:

no differences


Condition differences:

medium:Esculetin (50 ug/mL)?

  • temperature:37C
  • medium:Esculetin (50 ug/mL)
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

rrsG CHEBI:490095 Esculetin

OMP_AN:7624

OMP:0007080

altered gene expression

Relative to: OMP_AN:7599


Genotype differences:

no differences


Condition differences:

medium:Coumarin (50 ug/mL)?

  • temperature:37C
  • medium:Coumarin (50 ug/mL)
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:Q7DH26 stx1 CHEBI:28794 Coumarin

OMP_AN:7625

OMP:0007080

altered gene expression

Relative to: OMP_AN:7599


Genotype differences:

no differences


Condition differences:

medium:Umbelliferone (50 ug/mL)?

  • temperature:37C
  • medium:LB medium
  • medium:Umbelliferone (50 ug/mL)
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:Q7DH26 stx1 CHEBI:27510 Umbelliferone

OMP_AN:7626

OMP:0007080

altered gene expression

Relative to: OMP_AN:7599


Genotype differences:

no differences


Condition differences:

medium:Esculetin (50 ug/mL)?

  • temperature:37C
  • medium:Esculetin (50 ug/mL)
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:24837471[1]

UniProtKB:Q7DH26 stx1 CHEBI:490095 Esculetin

OMP_AN:7627

OMP:0000176

biofilm phenotype

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • other:96-well plate
  • temperature:37C
  • medium:LB medium
ECO:0000181

in vitro assay evidence

PMID:22047137[2]

Crystal violet biofilm assay



Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 1.46 1.47 1.48 Lee, JH et al. () Coumarins reduce biofilm formation and the virulence of Escherichia coli O157:H7. Phytomedicine 21 1037-42 PubMed OMPwiki page
  2. Lee, JH et al. (2011) Low concentrations of honey reduce biofilm formation, quorum sensing, and virulence in Escherichia coli O157:H7. Biofouling 27 1095-104 PubMed OMPwiki page