OMP ST:159 ! Escherichia coli K-12 MG1655

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 MG1655

Synonyms

CGSC #6300, ATCC 47076

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

F- Lambda- rph-1

Strain Reference
Strain availability

Coli Genetic Stock Center, ATCC

Ancestry
Annotated phenotypes




Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:357

OMP:0007253

presence of host colonization

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

ECO:0001027

colony counting evidence

PMID:17709419[2]

OMP_AN:1353

OMP:0000067

presence of cell motility by swarming

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • temperature:37C
  • medium:swarming motility medium
ECO:0005803

motility assay evidence

PMID:17391508[3]

OMP_AN:7646

OMP:0000253

gene expression phenotype

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • temperature:37C
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:22047137[4]

UniProtKB:P28307 csgA

OMP_AN:7647

OMP:0000253

gene expression phenotype

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • temperature:37C
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:22047137[4]

UniProtKB:P77257 lsrA

OMP_AN:7648

OMP:0000253

gene expression phenotype

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • temperature:37C
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:22047137[4]

UniProtKB:P76142 lsrB

OMP_AN:7649

OMP:0000253

gene expression phenotype

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • temperature:37C
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:22047137[4]

UniProtKB:P76143 lsrF

OMP_AN:7650

OMP:0000267

increased gene expression

Relative to: OMP_AN:7646


Genotype differences:

no differences


Condition differences:

medium:Acacia honey (0.5%)?

  • temperature:37C
  • medium:Acacia honey (0.5%)
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:22047137[4]

UniProtKB:P28307 csgA

OMP_AN:7651

OMP:0000267

increased gene expression

Relative to: OMP_AN:7647


Genotype differences:

no differences


Condition differences:

medium:Acacia honey (0.5%)?

  • temperature:37C
  • medium:Acacia honey (0.5%)
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:22047137[4]

UniProtKB:P77257 lsrA

OMP_AN:7652

OMP:0000267

increased gene expression

Relative to: OMP_AN:7648


Genotype differences:

no differences


Condition differences:

medium:Acacia honey (0.5%)?

  • temperature:37C
  • medium:Acacia honey (0.5%)
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:22047137[4]

UniProtKB:P76142 lsrB

OMP_AN:7653

OMP:0000267

increased gene expression

Relative to: OMP_AN:7649


Genotype differences:

no differences


Condition differences:

medium:Acacia honey (0.5%)?

  • temperature:37C
  • medium:Acacia honey (0.5%)
  • medium:LB medium
ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:22047137[4]

UniProtKB:P76143 lsrF

OMP_AN:7675

OMP:0000176

biofilm phenotype

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • other:96-well plate
  • temperature:37C
  • medium:LB medium
ECO:0000182

in vitro culture assay evidence

PMID:22047137[4]

Crystal violet biofilm assay

OMP_AN:7676

OMP:0007020

increased biofilm formation

Relative to: OMP_AN:7675


Genotype differences:

no differences


Condition differences:

medium:Honey (0.5%)?

  • other:96-well plate
  • temperature:37C
  • medium:Honey (0.5%)
  • medium:LB medium
ECO:0000182

in vitro culture assay evidence

PMID:22047137[4]

Crystal violet biofilm assay

OMP_AN:7677

OMP:0007020

increased biofilm formation

Relative to: OMP_AN:7675


Genotype differences:

no differences


Condition differences:

medium:Glucose (0.5%)?

  • other:96-well plate
  • temperature:37C
  • medium:Glucose (0.5%)
  • medium:LB medium
ECO:0000182

in vitro culture assay evidence

PMID:22047137[4]

CHEBI:17234 glucose

Crystal violet biofilm assay

OMP_AN:7678

OMP:0007020

increased biofilm formation

Relative to: OMP_AN:7675


Genotype differences:

no differences


Condition differences:

medium:Fructose (0.5%)?

  • other:96-well plate
  • temperature:37C
  • medium:Fructose (0.5%)
  • medium:LB medium
ECO:0000182

in vitro culture assay evidence

PMID:22047137[4]

CHEBI:28757 fructose

Crystal violet biofilm assay

OMP_AN:7679

OMP:0007020

increased biofilm formation

Relative to: OMP_AN:7675


Genotype differences:

no differences


Condition differences:

medium:Sucrose (0.5%)?

  • other:96-well plate
  • temperature:37C
  • medium:LB medium
  • medium:Sucrose (0.5%)
ECO:0000182

in vitro culture assay evidence

PMID:22047137[4]

CHEBI:17992 sucrose

Crystal violet biofilm assay

OMP_AN:7680

OMP:0007020

increased biofilm formation

Relative to: OMP_AN:7675


Genotype differences:

no differences


Condition differences:

medium:Lactose (0.5%)?

  • other:96-well plate
  • temperature:37C
  • medium:LB medium
  • medium:Lactose (0.5%)
ECO:0000182

PMID:22047137[4]

CHEBI:17716 lactose

Crystal violet biofilm assay

OMP_AN:7681

OMP:0007020

increased biofilm formation

Relative to: OMP_AN:7675


Genotype differences:

no differences


Condition differences:

medium:Galactose (0.5%)?

  • other:96-well plate
  • temperature:37C
  • medium:Galactose (0.5%)
  • medium:LB medium
ECO:0000182

in vitro culture assay evidence

PMID:22047137[4]

CHEBI:28260 galactose

Crystal violet biofilm assay

OMP_AN:7682

OMP:0007020

increased biofilm formation

Relative to: OMP_AN:7675


Genotype differences:

no differences


Condition differences:

medium:Methylglyoxal (0.001%)?

  • other:96-well plate
  • temperature:37C
  • medium:LB medium
  • medium:Methylglyoxal (0.001%)
ECO:0000182

in vitro culture assay evidence

PMID:22047137[4]

CHEBI:17158 methylglyoxal

Crystal violet biofilm assay

OMP_AN:7683

OMP:0007020

increased biofilm formation

Relative to: OMP_AN:7675


Genotype differences:

no differences


Condition differences:

medium:H2O2 (0.0005%)?

  • other:96-well plate
  • temperature:37C
  • medium:H2O2 (0.0005%)
  • medium:LB medium
ECO:0000182

in vitro culture assay evidence

PMID:22047137[4]

CHEBI:16240 H2O2

Crystal violet biofilm assay

OMP_AN:8278

OMP:0005337

absence of cell lysis

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • temperature:RT
  • medium:CPRG (20 ug/mL)
  • medium:IPTG (100 uM)
  • medium:LB medium
  • medium:NaCl (1%)
ECO:0000182

in vitro culture assay evidence

PMID:24391520[5]

OMP_AN:8307

OMP:0005337

absence of cell lysis

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • temperature:RT
  • medium:CPRG (20 ug/mL)
  • medium:IPTG (100 uM)
  • medium:LB medium
ECO:0000182

in vitro culture assay evidence

PMID:24391520[5]

OMP_AN:27250

OMP:0007181

presence of anaerobic respiration

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • medium:H2O2 (5 uM) as terminal electron acceptor
  • other:anoxic
  • temperature:37C
  • medium:glycerol minimal medium
ECO:0001165

colony counting evidence used in manual assertion

PMID:28696311[6]

CHEBI:16240 hydrogen peroxide

Figure 8

OMP_AN:27302

OMP:0007595

presence of population growth

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • temperature:37C
  • medium:LB broth
ECO:0001258

spectrophotometry evidence used in manual assertion

PMID:25777676[7]

Figure 1

OMP_AN:27303

OMP:0007595

presence of population growth

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • temperature:20C
  • medium:LB broth
ECO:0001258

spectrophotometry evidence used in manual assertion

PMID:25777676[7]

Figure 1

OMP_AN:37557

presence of growth at acidic pH

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • other:24 h incubation
  • pH:4.50
  • temperature:30C
  • medium:LB

ECO:0001847

PMID:27826291[8]

table 3

OMP_AN:37599

OMP:0007876

resistant to an antimicrobial agent

Relative to:


Genotype differences:

Nothing to compare


Condition differences:

Nothing to compare

  • temperature:37C
  • medium:LB
  • medium:streptolydigin (20 ug/ml)
ECO:0007699

cell growth assay evidence used in manual assertion

PMID:8244969[9]

CHEBI:45773 streptolydigin

E. coli K-12 is naturally resistant to streptolydigin, presumably because the drug is unable to cross the outer membrane.


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Jensen, KF (1993) The Escherichia coli K-12 "wild types" W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyrE expression levels. J. Bacteriol. 175 3401-7 PubMed OMPwiki page
  2. Autieri, SM et al. (2007) L-fucose stimulates utilization of D-ribose by Escherichia coli MG1655 DeltafucAO and E. coli Nissle 1917 DeltafucAO mutants in the mouse intestine and in M9 minimal medium. Infect. Immun. 75 5465-75 PubMed OMPwiki page
  3. Gómez-Gómez, JM et al. (2007) A novel role for RecA under non-stress: promotion of swarming motility in Escherichia coli K-12. BMC Biol. 5 14 PubMed OMPwiki page
  4. 4.00 4.01 4.02 4.03 4.04 4.05 4.06 4.07 4.08 4.09 4.10 4.11 4.12 4.13 4.14 4.15 4.16 Lee, JH et al. (2011) Low concentrations of honey reduce biofilm formation, quorum sensing, and virulence in Escherichia coli O157:H7. Biofouling 27 1095-104 PubMed OMPwiki page
  5. 5.0 5.1 Paradis-Bleau, C et al. (2014) A genome-wide screen for bacterial envelope biogenesis mutants identifies a novel factor involved in cell wall precursor metabolism. PLoS Genet. 10 e1004056 PubMed OMPwiki page
  6. Khademian, M & Imlay, JA (2017) cytochrome peroxidase is a respiratory oxidase that enables the use of hydrogen peroxide as a terminal electron acceptor. Proc. Natl. Acad. Sci. U.S.A. 114 E6922-E6931 PubMed OMPwiki page
  7. 7.0 7.1 Choudhury, P & Flower, AM (2015) Efficient assembly of ribosomes is inhibited by deletion of bipA in Escherichia coli. J. Bacteriol. 197 1819-27 PubMed OMPwiki page
  8. Vivijs, B et al. (2016) Identification of Genes Required for Growth of MG1655 at Moderately Low pH. Front Microbiol 7 1672 PubMed OMPwiki page
  9. Heisler, LM et al. (1993) Four contiguous amino acids define the target for streptolydigin resistance in the beta subunit of Escherichia coli RNA polymerase. J Biol Chem 268 25369-75 PubMed OMPwiki page