Difference between revisions of "OMP ST:159 ! Escherichia coli K-12 MG1655"

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OMP_AN:37599
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OMP:0007876
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resistant to an antimicrobial agent
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Relative to:
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<hr>Genotype differences:<br>
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Nothing to compare<br/>
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<hr>Condition differences:<br>
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Nothing to compare<br/>
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*temperature:37C
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*medium:LB
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*medium:streptolydigin (20 ug/ml)
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ECO:0007699
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cell growth assay evidence used in manual assertion
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PMID:8244969
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CHEBI:45773
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streptolydigin
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E. coli K-12 is naturally resistant to streptolydigin, presumably because the drug is unable to cross the outer membrane.
  
 
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Latest revision as of 16:12, 21 June 2021

Strain Summary

Strain Name

Escherichia coli K-12 MG1655

Synonyms

CGSC #6300, ATCC 47076

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

F- Lambda- rph-1

Strain Reference
Strain availability

Coli Genetic Stock Center, ATCC

Ancestry
Annotated phenotypes


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Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:357

OMP:0007253

presence of host colonization

Relative to:

Genotype differences:

Nothing to compare

Condition differences:

Nothing to compare

 ECO:0001027

colony counting evidence

PMID:17709419[2]

OMP_AN:1353

OMP:0000067

presence of cell motility by swarming

Relative to:

Genotype differences:

Nothing to compare

Condition differences:

Nothing to compare
  • temperature:37C
  • medium:swarming motility medium
 ECO:0005803

motility assay evidence

PMID:17391508[3]

OMP_AN:7646

OMP:0000253

gene expression phenotype

Relative to:

Genotype differences:

Nothing to compare

Condition differences:

Nothing to compare
  • temperature:37C
  • medium:LB medium
 ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:22047137[4]

UniProtKB:P28307 csgA

OMP_AN:7647

OMP:0000253

gene expression phenotype

Relative to:

Genotype differences:

Nothing to compare

Condition differences:

Nothing to compare
  • temperature:37C
  • medium:LB medium
 ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:22047137[4]

UniProtKB:P77257 lsrA

OMP_AN:7648

OMP:0000253

gene expression phenotype

Relative to:

Genotype differences:

Nothing to compare

Condition differences:

Nothing to compare
  • temperature:37C
  • medium:LB medium
 ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:22047137[4]

UniProtKB:P76142 lsrB

OMP_AN:7649

OMP:0000253

gene expression phenotype

Relative to:

Genotype differences:

Nothing to compare

Condition differences:

Nothing to compare
  • temperature:37C
  • medium:LB medium
 ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:22047137[4]

UniProtKB:P76143 lsrF

OMP_AN:7650

OMP:0000267

increased gene expression

Relative to: OMP_AN:7646

Genotype differences:

no differences

Condition differences:

medium:Acacia honey (0.5%)?

  • temperature:37C
  • medium:Acacia honey (0.5%)
  • medium:LB medium
 ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:22047137[4]

UniProtKB:P28307 csgA

OMP_AN:7651

OMP:0000267

increased gene expression

Relative to: OMP_AN:7647

Genotype differences:

no differences

Condition differences:

medium:Acacia honey (0.5%)?

  • temperature:37C
  • medium:Acacia honey (0.5%)
  • medium:LB medium
 ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:22047137[4]

UniProtKB:P77257 lsrA

OMP_AN:7652

OMP:0000267

increased gene expression

Relative to: OMP_AN:7648

Genotype differences:

no differences

Condition differences:

medium:Acacia honey (0.5%)?

  • temperature:37C
  • medium:Acacia honey (0.5%)
  • medium:LB medium
 ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:22047137[4]

UniProtKB:P76142 lsrB

OMP_AN:7653

OMP:0000267

increased gene expression

Relative to: OMP_AN:7649

Genotype differences:

no differences

Condition differences:

medium:Acacia honey (0.5%)?

  • temperature:37C
  • medium:Acacia honey (0.5%)
  • medium:LB medium
 ECO:0000109

reverse transcription polymerase chain reaction evidence

PMID:22047137[4]

UniProtKB:P76143 lsrF

OMP_AN:7675

OMP:0000176

biofilm phenotype

Relative to:

Genotype differences:

Nothing to compare

Condition differences:

Nothing to compare
  • other:96-well plate
  • temperature:37C
  • medium:LB medium
 ECO:0000182

in vitro culture assay evidence

PMID:22047137[4]

Crystal violet biofilm assay

OMP_AN:7676

OMP:0007020

increased biofilm formation

Relative to: OMP_AN:7675

Genotype differences:

no differences

Condition differences:

medium:Honey (0.5%)?

  • other:96-well plate
  • temperature:37C
  • medium:Honey (0.5%)
  • medium:LB medium
 ECO:0000182

in vitro culture assay evidence

PMID:22047137[4]

Crystal violet biofilm assay

OMP_AN:7677

OMP:0007020

increased biofilm formation

Relative to: OMP_AN:7675

Genotype differences:

no differences

Condition differences:

medium:Glucose (0.5%)?

  • other:96-well plate
  • temperature:37C
  • medium:Glucose (0.5%)
  • medium:LB medium
 ECO:0000182

in vitro culture assay evidence

PMID:22047137[4]

CHEBI:17234 glucose

Crystal violet biofilm assay

OMP_AN:7678

OMP:0007020

increased biofilm formation

Relative to: OMP_AN:7675

Genotype differences:

no differences

Condition differences:

medium:Fructose (0.5%)?

  • other:96-well plate
  • temperature:37C
  • medium:Fructose (0.5%)
  • medium:LB medium
 ECO:0000182

in vitro culture assay evidence

PMID:22047137[4]

CHEBI:28757 fructose

Crystal violet biofilm assay

OMP_AN:7679

OMP:0007020

increased biofilm formation

Relative to: OMP_AN:7675

Genotype differences:

no differences

Condition differences:

medium:Sucrose (0.5%)?

  • other:96-well plate
  • temperature:37C
  • medium:LB medium
  • medium:Sucrose (0.5%)
 ECO:0000182

in vitro culture assay evidence

PMID:22047137[4]

CHEBI:17992 sucrose

Crystal violet biofilm assay

OMP_AN:7680

OMP:0007020

increased biofilm formation

Relative to: OMP_AN:7675

Genotype differences:

no differences

Condition differences:

medium:Lactose (0.5%)?

  • other:96-well plate
  • temperature:37C
  • medium:LB medium
  • medium:Lactose (0.5%)
 ECO:0000182

PMID:22047137[4]

CHEBI:17716 lactose

Crystal violet biofilm assay

OMP_AN:7681

OMP:0007020

increased biofilm formation

Relative to: OMP_AN:7675

Genotype differences:

no differences

Condition differences:

medium:Galactose (0.5%)?

  • other:96-well plate
  • temperature:37C
  • medium:Galactose (0.5%)
  • medium:LB medium
 ECO:0000182

in vitro culture assay evidence

PMID:22047137[4]

CHEBI:28260 galactose

Crystal violet biofilm assay

OMP_AN:7682

OMP:0007020

increased biofilm formation

Relative to: OMP_AN:7675

Genotype differences:

no differences

Condition differences:

medium:Methylglyoxal (0.001%)?

  • other:96-well plate
  • temperature:37C
  • medium:LB medium
  • medium:Methylglyoxal (0.001%)
 ECO:0000182

in vitro culture assay evidence

PMID:22047137[4]

CHEBI:17158 methylglyoxal

Crystal violet biofilm assay

OMP_AN:7683

OMP:0007020

increased biofilm formation

Relative to: OMP_AN:7675

Genotype differences:

no differences

Condition differences:

medium:H2O2 (0.0005%)?

  • other:96-well plate
  • temperature:37C
  • medium:H2O2 (0.0005%)
  • medium:LB medium
 ECO:0000182

in vitro culture assay evidence

PMID:22047137[4]

CHEBI:16240 H2O2

Crystal violet biofilm assay

OMP_AN:8278

OMP:0005337

absence of cell lysis

Relative to:

Genotype differences:

Nothing to compare

Condition differences:

Nothing to compare
  • temperature:RT
  • medium:CPRG (20 ug/mL)
  • medium:IPTG (100 uM)
  • medium:LB medium
  • medium:NaCl (1%)
 ECO:0000182

in vitro culture assay evidence

PMID:24391520[5]

OMP_AN:8307

OMP:0005337

absence of cell lysis

Relative to:

Genotype differences:

Nothing to compare

Condition differences:

Nothing to compare
  • temperature:RT
  • medium:CPRG (20 ug/mL)
  • medium:IPTG (100 uM)
  • medium:LB medium
 ECO:0000182

in vitro culture assay evidence

PMID:24391520[5]

OMP_AN:27250

OMP:0007181

presence of anaerobic respiration

Relative to:

Genotype differences:

Nothing to compare

Condition differences:

Nothing to compare
  • medium:H2O2 (5 uM) as terminal electron acceptor
  • other:anoxic
  • temperature:37C
  • medium:glycerol minimal medium
 ECO:0001165

colony counting evidence used in manual assertion

PMID:28696311[6]

CHEBI:16240 hydrogen peroxide

Figure 8

OMP_AN:27302

OMP:0007595

presence of population growth

Relative to:

Genotype differences:

Nothing to compare

Condition differences:

Nothing to compare
  • temperature:37C
  • medium:LB broth
 ECO:0001258

spectrophotometry evidence used in manual assertion

PMID:25777676[7]

Figure 1

OMP_AN:27303

OMP:0007595

presence of population growth

Relative to:

Genotype differences:

Nothing to compare

Condition differences:

Nothing to compare
  • temperature:20C
  • medium:LB broth
 ECO:0001258

spectrophotometry evidence used in manual assertion

PMID:25777676[7]

Figure 1

OMP_AN:37557

presence of growth at acidic pH

Relative to:

Genotype differences:

Nothing to compare

Condition differences:

Nothing to compare
  • other:24 h incubation
  • pH:4.50
  • temperature:30C
  • medium:LB

ECO:0001847

PMID:27826291[8]

table 3

OMP_AN:37599

OMP:0007876

resistant to an antimicrobial agent

Relative to:

Genotype differences:

Nothing to compare

Condition differences:

Nothing to compare
  • temperature:37C
  • medium:LB
  • medium:streptolydigin (20 ug/ml)
 ECO:0007699

cell growth assay evidence used in manual assertion

PMID:8244969[9]

CHEBI:45773 streptolydigin

E. coli K-12 is naturally resistant to streptolydigin, presumably because the drug is unable to cross the outer membrane.

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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Jensen, KF (1993) The Escherichia coli K-12 "wild types" W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyrE expression levels. J. Bacteriol. 175 3401-7 PubMed OMPwiki page
  2. ↑ Autieri, SM et al. (2007) L-fucose stimulates utilization of D-ribose by Escherichia coli MG1655 DeltafucAO and E. coli Nissle 1917 DeltafucAO mutants in the mouse intestine and in M9 minimal medium. Infect. Immun. 75 5465-75 PubMed OMPwiki page
  3. ↑ Gómez-Gómez, JM et al. (2007) A novel role for RecA under non-stress: promotion of swarming motility in Escherichia coli K-12. BMC Biol. 5 14 PubMed OMPwiki page
  4. ↑ 4.00 4.01 4.02 4.03 4.04 4.05 4.06 4.07 4.08 4.09 4.10 4.11 4.12 4.13 4.14 4.15 4.16 Lee, JH et al. (2011) Low concentrations of honey reduce biofilm formation, quorum sensing, and virulence in Escherichia coli O157:H7. Biofouling 27 1095-104 PubMed OMPwiki page
  5. ↑ 5.0 5.1 Paradis-Bleau, C et al. (2014) A genome-wide screen for bacterial envelope biogenesis mutants identifies a novel factor involved in cell wall precursor metabolism. PLoS Genet. 10 e1004056 PubMed OMPwiki page
  6. ↑ Khademian, M & Imlay, JA (2017) cytochrome peroxidase is a respiratory oxidase that enables the use of hydrogen peroxide as a terminal electron acceptor. Proc. Natl. Acad. Sci. U.S.A. 114 E6922-E6931 PubMed OMPwiki page
  7. ↑ 7.0 7.1 Choudhury, P & Flower, AM (2015) Efficient assembly of ribosomes is inhibited by deletion of bipA in Escherichia coli. J. Bacteriol. 197 1819-27 PubMed OMPwiki page
  8. ↑ Vivijs, B et al. (2016) Identification of Genes Required for Growth of MG1655 at Moderately Low pH. Front Microbiol 7 1672 PubMed OMPwiki page
  9. ↑ Heisler, LM et al. (1993) Four contiguous amino acids define the target for streptolydigin resistance in the beta subunit of Escherichia coli RNA polymerase. J Biol Chem 268 25369-75 PubMed OMPwiki page
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