OMP ST:1102 ! Escherichia coli K-12 JW5592

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Strain Summary

Strain Name

Escherichia coli K-12 JW5592

Synonyms

JW5592-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 dapF755(del)::kan

Strain Reference

Coli Genetic Stock Center

Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes


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Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:1018

OMP:0007092

decreased cell motility by swarming

Relative to: OMP_AN:881

Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +dapF755(del) -IN(rrnD-rrnE)1

Condition differences:

no differences

  • temperature:33C
  • medium:swarming motility medium
 ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:36443

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27531

Genotype differences:

+lacZ4787(del)( +dapF755(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Ampicillin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:28971) Ampicillin

  • chemical concentration= 4 ug/ml
  • normalized fitness= -3.72

OMP_AN:36444

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27534

Genotype differences:

+lacZ4787(del)( +dapF755(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefoxitin sodium hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3501) Cefoxitin sodium hydrate

  • chemical concentration= 1 ug/ml
  • normalized fitness= -4.01

OMP_AN:36445

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27535

Genotype differences:

+lacZ4787(del)( +dapF755(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefsulodin sodium salt hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31380) Cefsulodin sodium salt hydrate

  • chemical concentration= 18 ug/ml
  • normalized fitness= -4.61

OMP_AN:36446

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27603

Genotype differences:

+lacZ4787(del)( +dapF755(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -5.83

OMP_AN:36447

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27613

Genotype differences:

+lacZ4787(del)( +dapF755(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -5.83

OMP_AN:36448

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27600

Genotype differences:

+lacZ4787(del)( +dapF755(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= -5.03

OMP_AN:36449

OMP:0007032

decreased utilization of inorganic nitrogen source

Relative to: OMP_AN:27610

Genotype differences:

+lacZ4787(del)( +dapF755(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= -5.03

OMP_AN:36450

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27558

Genotype differences:

+lacZ4787(del)( +dapF755(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:D-Cycloserine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:40009) D-Cycloserine

  • chemical concentration= 16 ug/ml
  • normalized fitness= -3.69

OMP_AN:36451

OMP:0005098

decreased resistance to UV radiation 

Relative to: OMP_AN:27619

Genotype differences:

+lacZ4787(del)( +dapF755(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:UV
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

  • chemical concentration= 18 sec
  • normalized fitness= -3.63
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Inoue, T et al. (2007) Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. J. Bacteriol. 189 950-7 PubMed OMPwiki page
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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