OMP ST:1088 ! Escherichia coli K-12 JW2408

From OMPwiki

Strain Summary

Strain Name

Escherichia coli K-12 JW2408

Synonyms

JW2408-2

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 ptsH744(del)::kan

Strain Reference

Coli Genetic Stock Center

Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes



Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:1000

OMP:0007092

decreased cell motility by swarming

Relative to: OMP_AN:881


Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +ptsH744(del) -IN(rrnD-rrnE)1


Condition differences:

no differences

  • temperature:33C
  • medium:swarming motility medium
ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:31652

OMP:0007896

decreased resistance to a surfactant

Relative to: OMP_AN:27581


Genotype differences:

+lacZ4787(del)( +ptsH744(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Benzalkonium chloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3020) Benzalkonium chloride

  • chemical concentration= 25 ug/ml
  • normalized fitness= -3.18

OMP_AN:31653

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27606


Genotype differences:

+lacZ4787(del)( +ptsH744(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= -3.84

OMP_AN:31654

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27616


Genotype differences:

+lacZ4787(del)( +ptsH744(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= -3.84

OMP_AN:31655

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27590


Genotype differences:

+lacZ4787(del)( +ptsH744(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Fosfomycin (CID:6093162) +Glucose-6-phosphate (CHEBI:4170)
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:6093162 + CHEBI:4170) Fosfomycin (CID:6093162) +Glucose-6-phosphate (CHEBI:4170)

  • chemical concentration= 0.05 ug/ml (in 50 mg/ml G6P)
  • normalized fitness= -3.54

OMP_AN:31656

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27603


Genotype differences:

+lacZ4787(del)( +ptsH744(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -4.4

OMP_AN:31657

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27613


Genotype differences:

+lacZ4787(del)( +ptsH744(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-Glucose anhydrous
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:42758) D-Glucose anhydrous

  • chemical concentration= 0.2%
  • normalized fitness= -4.4

OMP_AN:31658

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27605


Genotype differences:

+lacZ4787(del)( +ptsH744(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= -4.96

OMP_AN:31659

OMP:0007070

decreased organic carbon source utilization

Relative to: OMP_AN:27615


Genotype differences:

+lacZ4787(del)( +ptsH744(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:D-(+) Maltose monohydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:23615261) D-(+) Maltose monohydrate

  • chemical concentration= 0.1%
  • normalized fitness= -4.96

OMP_AN:31660

OMP:0006045

increased resistance to beta-lactam compound

Relative to: OMP_AN:27537


Genotype differences:

+lacZ4787(del)( +ptsH744(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Mecillinam
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:51208) Mecillinam

  • chemical concentration= 0.09 ug/ml
  • normalized fitness= 3.72

OMP_AN:31661

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27600


Genotype differences:

+lacZ4787(del)( +ptsH744(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= -4.33

OMP_AN:31662

OMP:0007032

decreased utilization of inorganic nitrogen source

Relative to: OMP_AN:27610


Genotype differences:

+lacZ4787(del)( +ptsH744(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:NH4Cl
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31206) NH4Cl

  • chemical concentration= 9.5mm
  • normalized fitness= -4.33

OMP_AN:31663

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27503


Genotype differences:

+lacZ4787(del)( +ptsH744(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nitrofurantoin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:71415) Nitrofurantoin

  • chemical concentration= 1.5 ug/ml
  • normalized fitness= -4.47

OMP_AN:31664

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27573


Genotype differences:

+lacZ4787(del)( +ptsH744(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Procaine hydrochloride
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8431) Procaine hydrochloride

  • chemical concentration= 10 mM
  • normalized fitness= -5.31

OMP_AN:31665

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27546


Genotype differences:

+lacZ4787(del)( +ptsH744(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Pyocyanin
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8653) Pyocyanin

  • chemical concentration= 0.2 ug/ml
  • normalized fitness= 3.26

OMP_AN:31666

OMP:0007912

decreased resistance to a chelator

Relative to: OMP_AN:27508


Genotype differences:

+lacZ4787(del)( +ptsH744(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:EGTA
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:30740) EGTA

  • chemical concentration= 2 mM
  • normalized fitness= -3.72

OMP_AN:31667

OMP:0006057

decreased resistance to aminoglycoside

Relative to: OMP_AN:27527


Genotype differences:

+lacZ4787(del)( +ptsH744(del) -lacZ4787(del)


Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Spectinomycin dihydrochloride pentahydrate
ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9217) Spectinomycin dihydrochloride pentahydrate

  • chemical concentration= 6 ug/ml
  • normalized fitness= -4.48


Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. Inoue, T et al. (2007) Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. J. Bacteriol. 189 950-7 PubMed OMPwiki page
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page