OMP ST:1030 ! Escherichia coli K-12 JW3607

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Strain Summary

Strain Name

Escherichia coli K-12 JW3607

Synonyms

JW3607-2

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 rfaQ743(del)::kan

Strain Reference

Coli Genetic Stock Center

Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes


edit table


Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:941

OMP:0007092

decreased cell motility by swarming

Relative to: OMP_AN:881

Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +rfaQ743(del) -IN(rrnD-rrnE)1

Condition differences:

no differences

  • temperature:33C
  • medium:swarming motility medium
 ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:1150

OMP:0005324

altered cell motility by swimming

Relative to: OMP_AN:882

Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +rfaQ743(del) -IN(rrnD-rrnE)1

Condition differences:

no differences

  • temperature:33C
  • medium:swimming motility medium
 ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:33888

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27594

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:64758 + CHEBI:8984) SDS (CHEBI:8984) +EDTA (CHEBI:64758)

  • chemical concentration= 0.5%/0.5mM %/0.5mM
  • normalized fitness= -3.58

OMP_AN:33889

OMP:0007959

increased population growth at low temperature

Relative to: OMP_AN:27620

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:
  • temperature:20C
  • medium37C
  • medium
  • temperature:20C
  • medium:LB agar
  • medium:Low temperature
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

  • chemical concentration= 20 C
  • normalized fitness= 4.04

OMP_AN:33890

OMP:0007954

decreased population growth at high temperature

Relative to: OMP_AN:27621

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:
  • temperature:45C
  • medium37C
  • medium
  • temperature:45C
  • medium:LB agar
  • medium:High temperature
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

  • chemical concentration= 45 C
  • normalized fitness= -4.25

OMP_AN:33891

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27575

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Acriflavine (acriflavinium chloride)
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:74728) Acriflavine (acriflavinium chloride)

  • chemical concentration= 2 ug/ml
  • normalized fitness= -3.54

OMP_AN:33892

OMP:0006036

decreased resistance to macrolide

Relative to: OMP_AN:27518

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azithromycin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:2955) Azithromycin

  • chemical concentration= 1 ug/ml
  • normalized fitness= -3.54

OMP_AN:33893

OMP:0007896

decreased resistance to a surfactant

Relative to: OMP_AN:27581

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Benzalkonium chloride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3020) Benzalkonium chloride

  • chemical concentration= 25 ug/ml
  • normalized fitness= -13.89

OMP_AN:33894

OMP:0006045

increased resistance to beta-lactam compound

Relative to: OMP_AN:27534

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefoxitin sodium hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3501) Cefoxitin sodium hydrate

  • chemical concentration= 0.75 ug/ml
  • normalized fitness= 3.99

OMP_AN:33895

OMP:0007173

decreased resistance to host defense peptides

Relative to: OMP_AN:27562

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cecropin B
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:16130488) Cecropin B

  • chemical concentration= 0.1 ug/ml
  • normalized fitness= -4.22

OMP_AN:33896

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27535

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cefsulodin sodium salt hydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:31380) Cefsulodin sodium salt hydrate

  • chemical concentration= 18 ug/ml
  • normalized fitness= -8.53

OMP_AN:33897

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27548

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Cerulenin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:171741) Cerulenin

  • chemical concentration= 6 ug/ml
  • normalized fitness= -4.21

OMP_AN:33898

OMP:0006037

increased resistance to macrolide

Relative to: OMP_AN:27519

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Clarithromycin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:3732) Clarithromycin

  • chemical concentration= 5 ug/ml
  • normalized fitness= 4.8

OMP_AN:33899

OMP:0007903

decreased resistance to bile acids

Relative to: OMP_AN:27586

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Deoxycholate sodium
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9177) Deoxycholate sodium

  • chemical concentration= 2 %
  • normalized fitness= -12.09

OMP_AN:33900

OMP:0007074

increased growth factor requirement

Relative to: OMP_AN:27606

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= 3.1

OMP_AN:33901

OMP:0007071

increased organic carbon source utilization

Relative to: OMP_AN:27616

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:M9
  • medium:N-Acetyl-D-glucosamine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:506227) N-Acetyl-D-glucosamine

  • chemical concentration= 0.15%
  • normalized fitness= 3.1

OMP_AN:33902

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27547

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Hydrogen peroxide
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:16240) Hydrogen peroxide

  • chemical concentration= 0.1 mM
  • normalized fitness= 3.76

OMP_AN:33903

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27546

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Pyocyanin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:8653) Pyocyanin

  • chemical concentration= 10 ug/ml
  • normalized fitness= -7.54

OMP_AN:33904

OMP:0007896

decreased resistance to a surfactant

Relative to: OMP_AN:27583

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Triton X-100
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9750) Triton X-100

  • chemical concentration= 0.03 %
  • normalized fitness= -5.78

OMP_AN:33905

OMP:0007878

increased resistance to an antimicrobial agent

Relative to: OMP_AN:27560

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Bacitracin Zinc Sulfate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:16219032) Bacitracin Zinc Sulfate

  • chemical concentration= 300 ug/ml
  • normalized fitness= 3.21

OMP_AN:33906

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27544

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Chloramphenicol
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:17698) Chloramphenicol

  • chemical concentration= 2 ug/ml
  • normalized fitness= -6.38

OMP_AN:33907

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27598

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= -5.02

OMP_AN:33908

OMP:0007886

decreased resistance to a metal

Relative to: OMP_AN:27608

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron excess-FeSO4 heptahydride
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride

  • chemical concentration= 1mM (MOPS; normal 100uM)
  • normalized fitness= -5.02

OMP_AN:33909

OMP:0007075

decreased growth factor requirement

Relative to: OMP_AN:27599

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= -3.01

OMP_AN:33910

OMP:0007937

decreased resistance to nutrient limitation

Relative to: OMP_AN:27609

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:MOPS
  • medium:Iron starvation-FeSO4·7H2O
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O

  • chemical concentration= 2uM (MOPS; normal 100uM)
  • normalized fitness= -3.01

OMP_AN:33911

OMP:0006040

decreased resistance to quinolone

Relative to: OMP_AN:27541

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Nalidixic acid sodium salt
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CID:3864541) Nalidixic acid sodium salt

  • chemical concentration= 2 ug/ml
  • normalized fitness= -8.34

OMP_AN:33912

OMP:0007010

decreased population growth at alkaline pH

Relative to: OMP_AN:27623

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:TAPS
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (**SID:24900259) TAPS

  • chemical concentration= pH 8
  • normalized fitness= -5.25

OMP_AN:33913

OMP:0007008

decreased population growth at acidic pH

Relative to: OMP_AN:27622

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:MES-HOMOPIPES
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (**SID:329817955 SID:57651422) MES-HOMOPIPES

  • chemical concentration= pH 4
  • normalized fitness= -4.56

OMP_AN:33914

OMP:0006057

decreased resistance to aminoglycoside

Relative to: OMP_AN:27527

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Spectinomycin dihydrochloride pentahydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9217) Spectinomycin dihydrochloride pentahydrate

  • chemical concentration= 4 ug/ml
  • normalized fitness= -3.92

OMP_AN:33915

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27591

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:45924 + CHEBI:9331) Trimethoprim (CHEBI:45924) + Sulfamethizole (CHEBI:9331)

  • chemical concentration= 0.1 + 50 ug/ml
  • normalized fitness= -4.25

OMP_AN:33916

OMP:0005097

increased resistance to UV radiation

Relative to: OMP_AN:27619

Genotype differences:

+lacZ4787(del)( +rfaQ743(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:UV
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

  • chemical concentration= 12 sec
  • normalized fitness= 3.82
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ 1.0 1.1 Inoue, T et al. (2007) Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. J. Bacteriol. 189 950-7 PubMed OMPwiki page
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 2.22 2.23 2.24 2.25 2.26 2.27 2.28 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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