OMP ST:801 ! Escherichia coli K-12 SMR4562
Contents
Strain Summary
| Strain Name |
SMR4562 |
|---|---|
| Synonyms |
FC40 (independent construction) |
| Taxon Information |
|
| Genotype |
(F' lacI33-Omega-lacZ proA+B+) Lambda- DE(gpt-lac)5 ara-600 thiE1 RifR |
| Strain Reference | |
| Strain availability | |
| Ancestry | |
| Annotated phenotypes |
|
Notes
SMR4562 is an independent construction of strain FC40 (Table S4[1]).
DE(gpt-lac)5 is the deletion DE( pro-lac)X111 of Jacob and originated in strain Hfr 3000 X111 (CGSC).
The lacI33-Omega-lacZ fusion on the F' plasmid contains all but the last 4 codons of lacI fused to codon 24 of lacZ. There is a polar +1 polar frameshift mutation at codon 320 of lacI, such that the cells are phenotypically Lac−. There is constitutive transcription of the hybrid gene from the lacIQ promoter ([2],[3] cited in [4]).
Phenotype Annotation
| Annotation ID | Qualifier | OMP ID | OMP term name | Relative phenotype information | Experimental condition | ECO ID | ECO term name | Reference | Annotation Extension | Notes |
|---|---|---|---|---|---|---|---|---|---|---|
|
OMP_AN:115 |
OMP:0005032 |
presence of stress-induced mutagenesis |
Relative to: Genotype differences: Nothing to compare Condition differences: Nothing to compare |
|
ECO:0000182 |
in vitro culture assay evidence |
colony papillation assay | |||
Additional Information
Links
References
See Help:References for how to manage references in OMPwiki.
- ↑ 1.0 1.1 1.2 Al Mamun, AA et al. (2012) Identity and function of a large gene network underlying mutagenic repair of DNA breaks. Science 338 1344-8 PubMed OMPwiki page
- ↑ Müller-Hill, B & Kania, J (1974) Lac repressor can be fused to beta-galactosidase. Nature 249 561-3 PubMed OMPwiki page
- ↑ Calos, MP & Miller, JH (1981) Genetic and sequence analysis of frameshift mutations induced by ICR-191. J. Mol. Biol. 153 39-64 PubMed OMPwiki page
- ↑ Cairns, J & Foster, PL (1991) Adaptive reversion of a frameshift mutation in Escherichia coli. Genetics 128 695-701 PubMed OMPwiki page
