OMP ST:74708 ! Escherichia coli K-12 CAG41434

Strain Summary

Strain Name	Escherichia coli K-12 CAG41434
Synonyms
Taxon Information	Pangenome: Escherichia coli Subspecies and/or strain: K-12 NCBI Taxonomy ID: 83333
Genotype	F- Lambda- rph-1 Lamda(rpoHP3::lacZ) lacX74(del) yfgL(del)::Km CmR
Strain Reference	PMID:15968066
Strain availability
Ancestry	parent:OMP ST:74707 ! Escherichia coli K-12 CAG45114
Annotated phenotypes
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Notes

In order to confirm that the yfgL(del) deletion was responsible for the sensitivity to certain antimicrobial agents, a plasmid carrying the yfgL gene was inserted into this strain. The two transformants, CAG41350 and CAG41517, were tested and the EOP experiment was repeated using these transformants. After introducing the plasmids, the transformants displayed the wild-type phenotype.

Phenotype Annotation

Annotation ID	Qualifier	OMP ID	OMP term name	Relative phenotype information	Experimental condition	ECO ID	ECO term name	Reference	Annotation Extension	Notes
OMP_AN:27320	NOT	OMP:0007894	altered resistance to a surfactant	Relative to: OMP_AN:27317 Genotype differences: +yfgL(del) +CmR Condition differences: no differences	temperature:30C medium:LB broth medium:SDS (3.5%)	ECO:0001165	colony counting evidence used in manual assertion	PMID:15968066	CHEBI:8984 SDS	Table 5; EOP=0.4
OMP_AN:27321		OMP:0007221	abolished resistance to rifamycin	Relative to: OMP_AN:27318 Genotype differences: +yfgL(del) +CmR Condition differences: no differences	temperature:30C medium:LB broth medium:rifampicin (5 mg/ml)	ECO:0001165	colony counting evidence used in manual assertion	PMID:15968066	CHEBI:28077 rifampicin	Table 5; EOP=6E-9
OMP_AN:27322	NOT	OMP:0007134	altered resistance to an antimicrobial agent	Relative to: OMP_AN:27319 Genotype differences: +yfgL(del) +CmR Condition differences: no differences	temperature:30C medium:LB broth medium:novobiocin (150 mg/ml)	ECO:0001165	colony counting evidence used in manual assertion	PMID:15968066	CHEBI:28368 novobiocin	Table 5; EOP=0.3
OMP_AN:27334	NOT	OMP:0007901	altered resistance to bile acids	Relative to: OMP_AN:27332 Genotype differences: +yfgL(del) +CmR Condition differences: no differences	temperature:30C medium:MacConkey agar	ECO:0001165	colony counting evidence used in manual assertion	PMID:15968066
OMP_AN:27343	NOT	OMP:0007001	altered motility	Relative to: OMP_AN:27340 Genotype differences: +yfgL(del) +CmR Condition differences: no differences	temperature:37C medium:LB soft agar	ECO:0007192	motility assay evidence used in manual assertion	PMID:15968066
OMP_AN:27344	NOT	OMP:0007001	altered motility	Relative to: OMP_AN:27339 Genotype differences: +yfgL(del) +CmR Condition differences: no differences	temperature:30C medium:LB soft agar	ECO:0007192	motility assay evidence used in manual assertion	PMID:15968066
OMP_AN:27353	NOT	OMP:0007519	altered population growth in high osmolarity	Relative to: OMP_AN:27351 Genotype differences: +yfgL(del) +CmR Condition differences: no differences	temperature:30C medium:Low-osmolarity medium medium:NaCl	ECO:0001258	spectrophotometry evidence used in manual assertion	PMID:15968066	CHEBI:26710 NaCl	The low-osmolarity medium was supplemented with 0.1 M NaCl, 0.2 M NaCl, 0.3 M NaCl, 0.4 M NaCl
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References

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