OMP ST:71046 ! Escherichia coli K-12 BW25113 cydC::SPA-kan

Strain Summary

Strain Name	Escherichia coli K-12 BW25113 cydC::SPA-kan
Synonyms
Taxon Information	Pangenome: Escherichia coli Subspecies and/or strain: K-12 NCBI Taxonomy ID: 83333
Genotype	DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 cydC::SPA-kan
Strain Reference	PMID:21185072
Strain availability
Ancestry	parent:OMP ST:800 ! Escherichia coli K-12 BW25113
Annotated phenotypes
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Phenotype Annotation

Annotation ID	Qualifier	OMP ID	OMP term name	Relative phenotype information	Experimental condition	ECO ID	ECO term name	Reference	Annotation Extension	Notes
OMP_AN:37207		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27594 Genotype differences: +lacZ4787(del)( +cydC -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:SDS (CHEBI:8984) +EDTA (CHEBI:64758)	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:64758 + CHEBI:8984) SDS (CHEBI:8984) +EDTA (CHEBI:64758)	chemical concentration= 0.5%/0.5mM %/0.5mM normalized fitness= -3.85
OMP_AN:37208		OMP:0007927	decreased anaerobic population growth	Relative to: OMP_AN:27618 Genotype differences: +lacZ4787(del)( +cydC -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Anaerobic	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072		normalized fitness= -3.64
OMP_AN:37209		OMP:0007903	decreased resistance to bile acids	Relative to: OMP_AN:27587 Genotype differences: +lacZ4787(del)( +cydC -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:26711 + CHEBI:9177) Bile salts chemical-Mixture of sodium cholate (CHEBI:26711) and sodium deoxycholate (CHEBI:9177)	chemical concentration= 2 % normalized fitness= -3.6
OMP_AN:37210		OMP:0007250	increased resistance to a chemical	Relative to: OMP_AN:27571 Genotype differences: +lacZ4787(del)( +cydC -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:CCCP (Carbonyl cyanide 3-chlorophenylhydrazone)	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:3259) CCCP (Carbonyl cyanide 3-chlorophenylhydrazone)	chemical concentration= 2 ug/ml normalized fitness= 3.51
OMP_AN:37211		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27514 Genotype differences: +lacZ4787(del)( +cydC -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Chlorpromazine hydrochloride	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:3649) Chlorpromazine hydrochloride	chemical concentration= 6 uM normalized fitness= -8.24
OMP_AN:37212		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27545 Genotype differences: +lacZ4787(del)( +cydC -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Isoniazid	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:6030) Isoniazid	chemical concentration= 1.5 mM normalized fitness= -5.08
OMP_AN:37213		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27510 Genotype differences: +lacZ4787(del)( +cydC -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Mitomycin C	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:27504) Mitomycin C	chemical concentration= 0.1 ug/ml normalized fitness= -5.6
OMP_AN:37214		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27570 Genotype differences: +lacZ4787(del)( +cydC -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Propidium iodide	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:51240) Propidium iodide	chemical concentration= 20 ug/ml normalized fitness= -5.69
OMP_AN:37215		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27546 Genotype differences: +lacZ4787(del)( +cydC -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Pyocyanin	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:8653) Pyocyanin	chemical concentration= 10 ug/ml normalized fitness= -8.04
OMP_AN:37216		OMP:0007249	decreased resistance to a chemical	Relative to: OMP_AN:27567 Genotype differences: +lacZ4787(del)( +cydC -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Theophylline anhydrous	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:28177) Theophylline anhydrous	chemical concentration= 100 ug/ml normalized fitness= -3.48
OMP_AN:37217		OMP:0007879	decreased resistance to an antimicrobial agent	Relative to: OMP_AN:27589 Genotype differences: +lacZ4787(del)( +cydC -lacZ4787(del) Condition differences: no differences	temperature:37C medium:LB agar medium:Cefsulodin + Mecillinam	ECO:0007005	high throughput direct assay evidence used in manual assertion	PMID:21185072	towards some (CHEBI:31380 + CHEBI:51208) Cefsulodin + Mecillinam	chemical concentration= 6 + 0.03 ug/ml normalized fitness= -4.42
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References

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