id: ECO:0005529
name: random mutagenesis evidence
def: "A type of experimental phenotypic evidence where radiation or a DNA-damaging agent in vivo, or PCR with degenerate primers in vitro are used for non-targeted mutagenesis." [ECO:SW, PMID:15153637, PMID:18265275]
is_a: ECO:0000059 ! experimental phenotypic evidence
comment: Random mutagenesis is not often used in TF-binding site determination; however, it is sometimes used to investigate the effect of mutations in TF binding domains in conjunction with expression assays. Random mutations can also be introduced via chemicals, error-prone PCR (EP-PCR), degenerate oligonucleotides-Pfu(DOP), UV irradiation, mutator strains, nucleotide analogs, or DNA recombination.
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Last version checked releases/2019-06-14.
14:06:2019 08:25.
rctauber | Last updated releases/2018-09-14 | |
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