Category:ECO:0000234 ! 4C evidence

name: 4C evidence
def: "A type of chromosome conformation-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a prior circularized 3C library followed by product quantitation with a microarray." [ECO:MCC, PMID:17033624]
synonym: "chromosome conformation capture on chip" EXACT []
synonym: "circularized 3C" EXACT []
synonym: "circularized chromosome conformation capture evidence" EXACT []
is_a: ECO:0000232 ! chromosome conformation-based evidence
comment: 4C is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 4C differs only in the fifth step, which involves inverse polymerase chain reaction (inverse PCR) on a circularized 3C library prior to product quantitation.