Category:L-salts medium
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Purpose
A minimal medium used by E.R. Leadbetter and J.W. Foster for cultivation of methane-utilizing bacteria.[1] For growth of methanotrophs the L medium was incubated in an atmosphere of methane mixed with air.[1]
Technical Details
Materials Needed
Per 1 L deionized water:
- NaNO3, 2.0 g
- MgSO4.7H2O, 0.2 g
- FeSO4.7H2O, 1.0 mg
- Na2HPO4, 0.2 lg ?
- NaH2PO4, 0.09 g
- Cu (as CuSO4.5H2O), 5 ug
- B (as H3BO3), 10 ug
- Mn (as MnSO4.5H2O), 10 ug
- Zn (as ZnSO4.7H2O), 70 ug
- Mo (as MoO3), 10 ug
- KCl, 0.04 g
- CaCl2, 0.015 g
Methods
Examples
Notes
References
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- ↑ 1.0 1.1 LEADBETTER, ER & FOSTER, JW (1958) Studies on some methane-utilizing bacteria. Arch Mikrobiol 30 91-118 PubMed OMPwiki page
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