Category:Hektoen Enteric Agar
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Purpose
- To recover gastrointestinal pathogens, such as Salmonella and Shigella, from clinical specimens[1][2]
- Inhibits most nonpathogenic enteric organisms
Technical Details
- The presence of bile salts and dye inhibit the growth of most Gram-positive bacteria
- Lactose, sucrose, and salicin are carbohydrates in the medium
- Strains (generally nonpathogenic) that are able to utilize any of these sugars appear as orange-yellow colonies
- Strains that strictly utilize salicin will appear salmon-pink to orange-yellow
- Strains unable to utilize any of these and will appear as translucent, light green, or blueish-green (i.e. Salmonella and Shigella)
- The addition of thiosulfate and ferric ammonium citrate can detect the production of H2S
- Colonies appear black or have black centers (e.g. Salmonella)[3]
Materials Needed
- Pre-mixed powder is available for purchase. The components are listed below.[4]
- Proteose, peptone
- Yeast extract
- Bile salts no.3
- Lactose
- Saccharose
- Salicin
- Sodium chloride
- Sodium thiosulfate
- Ferric ammonium citrate
- Acid fuchsin
- Bromthymol blue
- Agar
Methods
- Specific information on preparing these plates can be found here.
Examples
Notes
- Salmonella serovar Typhi is a weak H2S producer, so may not appear black
- Hektoen Agar was named after the institute where studies were conducted[1]
References
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- ↑ 1.0 1.1 King, S & Metzger, WI (1968) A new plating medium for the isolation of enteric pathogens. I. hektoen enteric agar. Appl Microbiol 16 577-8 PubMed OMPwiki page
- ↑ King, S & Metzger, WI (1968) A new plating medium for the isolation of enteric pathogens. II. Comparison of hektoen enteric agar with SS and EMB agar. Appl Microbiol 16 579-81 PubMed OMPwiki page
- ↑ Winn, W., Jr., et al. 2006. Konemann’s color atlas and diagnostic text of microbiology, 6th ed., p. 212–302. Lippencott Williams & Wilkins Publishers, Philadelphia, PA.
- ↑ Difco. 1984. Difco manual, 10th ed. Difco Laboratories, Detroit, MI.
Pages in category "Hektoen Enteric Agar"
The following 2 pages are in this category, out of 2 total.
