Category:ECO:0006077 ! hybrid interaction evidence used in manual assertion

name: hybrid interaction evidence used in manual assertion
def: "A type of affinity evidence that is used in manual assertion where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein." [ECO:MCC]
is_a: ECO:0000025 ! hybrid interaction evidence
is_a: ECO:0006075 ! affinity evidence used in manual assertion
relationship: used_in: ECO:0000218 ! manual assertion
intersection_of: ECO:0000025 ! hybrid interaction evidence
intersection_of: used_in ECO:0000218 ! manual assertion